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Magnesium in PDB 1b8j: Alkaline Phosphatase Complexed with Vanadate

Enzymatic activity of Alkaline Phosphatase Complexed with Vanadate

All present enzymatic activity of Alkaline Phosphatase Complexed with Vanadate:
3.1.3.1;

Protein crystallography data

The structure of Alkaline Phosphatase Complexed with Vanadate, PDB code: 1b8j was solved by K.M.Holtz, B.Stec, E.R.Kantrowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 1.90
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 195.410, 167.810, 76.520, 90.00, 90.00, 90.00
R / Rfree (%) 17.7 / 19.6

Other elements in 1b8j:

The structure of Alkaline Phosphatase Complexed with Vanadate also contains other interesting chemical elements:

Zinc (Zn) 4 atoms
Vanadium (V) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Alkaline Phosphatase Complexed with Vanadate (pdb code 1b8j). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Alkaline Phosphatase Complexed with Vanadate, PDB code: 1b8j:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 1b8j

Go back to Magnesium Binding Sites List in 1b8j
Magnesium binding site 1 out of 2 in the Alkaline Phosphatase Complexed with Vanadate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Alkaline Phosphatase Complexed with Vanadate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg452

b:14.8
occ:1.00
OD2 A:ASP51 1.9 22.7 1.0
O A:HOH1474 2.0 16.9 1.0
O A:HOH1473 2.1 11.5 1.0
OE2 A:GLU322 2.2 10.8 1.0
OG1 A:THR155 2.3 6.9 1.0
O A:HOH1475 2.3 17.1 1.0
CG A:ASP51 3.1 20.2 1.0
CB A:THR155 3.2 3.4 1.0
CD A:GLU322 3.3 10.3 1.0
OE1 A:GLU322 3.5 12.2 1.0
OD2 A:ASP153 3.7 17.3 1.0
CB A:ASP51 3.7 18.7 1.0
N A:THR155 4.1 6.4 1.0
OD1 A:ASP51 4.1 23.4 1.0
O1 A:SVA102 4.1 44.8 1.0
O A:HOH1472 4.2 16.0 1.0
O A:HOH1471 4.2 19.2 1.0
CG2 A:THR155 4.2 3.0 1.0
CA A:THR155 4.3 7.8 1.0
CB A:SVA102 4.5 8.6 1.0
ZN A:ZN451 4.6 20.1 1.0
CG A:GLU322 4.6 5.0 1.0
CB A:ALA324 4.6 12.8 1.0
OG A:SVA102 4.7 34.5 1.0
CG A:ASP153 4.7 18.8 1.0
OD2 A:ASP369 4.8 16.1 1.0
O A:ALA324 4.9 24.6 1.0
CA A:ALA324 4.9 13.7 1.0

Magnesium binding site 2 out of 2 in 1b8j

Go back to Magnesium Binding Sites List in 1b8j
Magnesium binding site 2 out of 2 in the Alkaline Phosphatase Complexed with Vanadate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Alkaline Phosphatase Complexed with Vanadate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg452

b:21.4
occ:1.00
OD2 B:ASP51 2.0 24.9 1.0
OE2 B:GLU322 2.0 21.0 1.0
O B:HOH1409 2.1 20.8 1.0
O B:HOH1410 2.1 21.9 1.0
O B:HOH1411 2.2 24.3 1.0
OG1 B:THR155 2.2 8.6 1.0
CG B:ASP51 3.1 24.6 1.0
CD B:GLU322 3.1 23.3 1.0
CB B:THR155 3.3 9.4 1.0
OE1 B:GLU322 3.5 25.1 1.0
CB B:ASP51 3.6 21.8 1.0
OD2 B:ASP153 3.9 25.8 1.0
OD1 B:ASP51 4.1 28.8 1.0
N B:THR155 4.1 14.6 1.0
O B:HOH1407 4.2 19.2 1.0
CG2 B:THR155 4.3 9.9 1.0
CA B:THR155 4.3 13.7 1.0
CG B:GLU322 4.4 15.2 1.0
O1 B:SVA102 4.4 36.2 1.0
O B:HOH1320 4.5 45.3 1.0
CB B:SVA102 4.5 8.1 1.0
CB B:ALA324 4.6 14.7 1.0
ZN B:ZN451 4.6 23.3 1.0
O B:ALA324 4.7 16.5 1.0
CG B:ASP153 4.7 21.1 1.0
CA B:ALA324 4.7 16.3 1.0
OG B:SVA102 4.7 42.2 1.0
OD2 B:ASP369 4.8 12.8 1.0
CA B:ASP51 5.0 13.5 1.0

Reference:

K.M.Holtz, B.Stec, E.R.Kantrowitz. A Model of the Transition State in the Alkaline Phosphatase Reaction. J.Biol.Chem. V. 274 8351 1999.
ISSN: ISSN 0021-9258
PubMed: 10085061
DOI: 10.1074/JBC.274.13.8351
Page generated: Mon Dec 14 03:47:16 2020

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