Magnesium in the structure of Alkaline Phosphatase Complexed With Vanadate (pdb 1b8j)

The binding sites of Magnesium atom in the structure of Alkaline Phosphatase Complexed With Vanadate (pdb code 1b8j). This binding sites where shown with 5.0 Angstroms radius around Magnesium atom. The 1b8j structure was solved by K.M.HOLTZ, B.STEC, E.R.KANTROWITZ, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 8.0-1.9 Space group I222 a (A) 195.410 b (A) 167.810 c (A) 76.520 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 17.7 Rfree (%) 19.6 Magnesium Binding Sites: Magnesium binding site 1 out of 2 in 1b8j Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Magnesium in the PDB 1b8j. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Magnesium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Asp51, A: Sva102, A: Asp153, A: Thr155, A: Glu322, A: Ala324, A: Asp369, A: Zn451, A: Hoh1471, A: Hoh1472, A: Hoh1473, A: Hoh1474, A: Hoh1475, conact list: Atom Atom Distance (A) Mg CB A:Asp51 3.74 Mg OD2 A:Asp51 1.92 Mg OD1 A:Asp51 4.07 Mg CG A:Asp51 3.06 Mg CB A:Sva102 4.50 Mg O1 A:Sva102 4.09 Mg OG A:Sva102 4.66 Mg OD2 A:Asp153 3.71 Mg CG A:Asp153 4.67 Mg N A:Thr155 4.05 Mg CB A:Thr155 3.19 Mg CG2 A:Thr155 4.22 Mg OG1 A:Thr155 2.27 Mg CA A:Thr155 4.26 Mg OE1 A:Glu322 3.54 Mg OE2 A:Glu322 2.24 Mg CD A:Glu322 3.28 Mg CG A:Glu322 4.58 Mg O A:Ala324 4.85 Mg CB A:Ala324 4.65 Mg CA A:Ala324 4.92 Mg OD2 A:Asp369 4.80 Mg ZN A:Zn451 4.56 Mg O A:Hoh1471 4.20 Mg O A:Hoh1472 4.17 Mg O A:Hoh1473 2.11 Mg O A:Hoh1474 2.00 Mg O A:Hoh1475 2.32 interactive model: Magnesium binding site 2 out of 2 in 1b8j Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Magnesium in the PDB 1b8j. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Magnesium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Asp51, B: Sva102, B: Asp153, B: Thr155, B: Glu322, B: Ala324, B: Asp369, B: Zn451, B: Hoh1320, B: Hoh1407, B: Hoh1409, B: Hoh1410, B: Hoh1411, conact list: Atom Atom Distance (A) Mg CB B:Asp51 3.63 Mg OD2 B:Asp51 2.02 Mg OD1 B:Asp51 4.09 Mg CG B:Asp51 3.06 Mg CA B:Asp51 4.97 Mg CB B:Sva102 4.54 Mg O1 B:Sva102 4.39 Mg OG B:Sva102 4.75 Mg OD2 B:Asp153 3.85 Mg CG B:Asp153 4.70 Mg N B:Thr155 4.09 Mg CB B:Thr155 3.26 Mg CG2 B:Thr155 4.25 Mg OG1 B:Thr155 2.23 Mg CA B:Thr155 4.30 Mg OE1 B:Glu322 3.51 Mg OE2 B:Glu322 2.04 Mg CD B:Glu322 3.13 Mg CG B:Glu322 4.39 Mg O B:Ala324 4.68 Mg CB B:Ala324 4.56 Mg CA B:Ala324 4.73 Mg OD2 B:Asp369 4.79 Mg ZN B:Zn451 4.59 Mg O B:Hoh1320 4.47 Mg O B:Hoh1407 4.18 Mg O B:Hoh1409 2.09 Mg O B:Hoh1410 2.13 Mg O B:Hoh1411 2.21 interactive model: