Magnesium in PDB 1b8n: Purine Nucleoside Phosphorylase

All present enzymatic activity of Purine Nucleoside Phosphorylase:
2.4.2.1;

The structure of Purine Nucleoside Phosphorylase, PDB code: 1b8n was solved by A.A.Fedorov, G.A.Kicska, E.V.Fedorov, B.V.Strokopytov, P.C.Tyler, R.H.Furneaux, V.L.Schramm, S.C.Almo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.00
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	90.320, 90.320, 90.320, 90.00, 90.00, 90.00
R / Rfree (%)	16.4 / 24.6

The binding sites of Magnesium atom in the Purine Nucleoside Phosphorylase (pdb code 1b8n). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Purine Nucleoside Phosphorylase, PDB code: 1b8n:

Magnesium binding site 1 out of 1 in the Purine Nucleoside Phosphorylase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Purine Nucleoside Phosphorylase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg598 b:22.7 occ:1.00 O A:HOH357 2.2 24.0 1.0 O A:HOH321 2.2 13.3 1.0 O A:HOH327 2.2 19.3 1.0 O A:HOH305 2.2 24.5 1.0 O A:HOH341 2.3 30.8 1.0 O A:HOH331 2.3 39.3 1.0 OE2 A:GLU22 4.0 30.0 1.0 O A:HOH403 4.1 41.2 1.0 OE1 A:GLU22 4.2 26.4 1.0 O A:GLN44 4.3 28.9 1.0 OE1 A:GLN46 4.4 21.5 1.0 O A:ALA45 4.5 21.5 1.0 CD A:GLU22 4.5 28.2 1.0 CB A:GLN46 4.6 16.9 1.0 C A:GLN44 4.8 29.2 1.0

G.A.Kicska, P.C.Tyler, G.B.Evans, R.H.Furneaux, W.Shi, A.A.Fedorov, A.Lewandowicz, S.M.Cahill, S.C.Almo, V.L.Schramm. Atomic Dissection of the Hydrogen Bond Network For Transition-State Analogue Binding to Purine Nucleoside Phosphorylase Biochemistry V. 41 14489 2002.
ISSN: ISSN 0006-2960
PubMed: 12463747
DOI: 10.1021/BI026636F