Magnesium in PDB 1bh2: A326S Mutant of An Inhibitory Alpha Subunit

The structure of A326S Mutant of An Inhibitory Alpha Subunit, PDB code: 1bh2 was solved by M.B.Mixon, B.A.Posner, M.A.Wall, A.G.Gilman, S.R.Sprang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.00 / 2.10
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	79.584, 79.584, 105.556, 90.00, 90.00, 120.00
R / Rfree (%)	19 / 25

The binding sites of Magnesium atom in the A326S Mutant of An Inhibitory Alpha Subunit (pdb code 1bh2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the A326S Mutant of An Inhibitory Alpha Subunit, PDB code: 1bh2:

Magnesium binding site 1 out of 1 in the A326S Mutant of An Inhibitory Alpha Subunit


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of A326S Mutant of An Inhibitory Alpha Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg357 b:30.3 occ:0.81 O A:HOH413 2.0 18.0 1.0 OG A:SER47 2.1 20.1 1.0 O A:HOH532 2.1 21.6 1.0 OG1 A:THR181 2.2 31.8 1.0 O2G A:GSP355 2.2 29.6 0.9 O2B A:GSP355 2.2 16.3 0.9 CB A:SER47 3.1 17.9 1.0 CB A:THR181 3.1 31.0 1.0 PB A:GSP355 3.3 15.3 0.9 PG A:GSP355 3.4 32.7 0.9 O3B A:GSP355 3.5 23.1 0.9 OD2 A:ASP200 3.9 25.2 1.0 N A:SER47 4.0 17.1 1.0 N A:THR181 4.0 31.1 1.0 CA A:SER47 4.1 16.6 1.0 O3G A:GSP355 4.1 30.6 0.9 O2A A:GSP355 4.1 20.5 0.9 CA A:THR181 4.1 32.5 1.0 CG2 A:THR181 4.2 28.4 1.0 OD1 A:ASP200 4.2 25.6 1.0 O3A A:GSP355 4.3 14.2 0.9 O1B A:GSP355 4.4 14.6 0.9 O A:VAL179 4.4 21.9 1.0 CG A:ASP200 4.5 20.4 1.0 PA A:GSP355 4.6 17.5 0.9 O1A A:GSP355 4.8 16.3 0.9 O A:VAL201 4.9 36.0 1.0 C A:LYS180 4.9 29.4 1.0 S1G A:GSP355 5.0 34.5 0.9 CE A:LYS46 5.0 20.6 1.0

B.A.Posner, M.B.Mixon, M.A.Wall, S.R.Sprang, A.G.Gilman. The A326S Mutant of GIALPHA1 As An Approximation of the Receptor-Bound State. J.Biol.Chem. V. 273 21752 1998.
ISSN: ISSN 0021-9258
PubMed: 9705312
DOI: 10.1074/JBC.273.34.21752