Magnesium in PDB 1bmf: Bovine Mitochondrial F1-Atpase

All present enzymatic activity of Bovine Mitochondrial F1-Atpase:
3.6.1.34;

The structure of Bovine Mitochondrial F1-Atpase, PDB code: 1bmf was solved by J.P.Abrahams, A.G.W.Leslie, R.Lutter, J.E.Walker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.85
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	284.220, 107.760, 139.680, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 25.4

The binding sites of Magnesium atom in the Bovine Mitochondrial F1-Atpase (pdb code 1bmf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the Bovine Mitochondrial F1-Atpase, PDB code: 1bmf:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in the Bovine Mitochondrial F1-Atpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Bovine Mitochondrial F1-Atpase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:26.3 occ:1.00 O A:HOH686 2.0 31.9 1.0 O2G A:ANP600 2.1 28.3 1.0 O2B A:ANP600 2.2 25.2 1.0 OG1 A:THR176 2.3 26.2 1.0 CB A:THR176 3.3 24.7 1.0 PG A:ANP600 3.4 27.9 1.0 PB A:ANP600 3.4 26.4 1.0 N3B A:ANP600 3.5 26.9 1.0 N A:THR176 4.1 25.1 1.0 O A:HOH625 4.1 30.7 1.0 O3G A:ANP600 4.1 29.1 1.0 CG2 A:THR176 4.3 24.4 1.0 CA A:THR176 4.3 25.5 1.0 OD1 A:ASP269 4.3 30.0 1.0 OD2 A:ASP269 4.3 28.2 1.0 O3A A:ANP600 4.4 26.1 1.0 O1G A:ANP600 4.4 28.3 1.0 O2A A:ANP600 4.5 26.8 1.0 O1B A:ANP600 4.5 25.0 1.0 CG A:ASP269 4.7 28.0 1.0 PA A:ANP600 4.8 25.6 1.0 O1A A:ANP600 4.8 25.8 1.0 O A:HOH615 4.8 33.4 1.0

Magnesium binding site 2 out of 5 in the Bovine Mitochondrial F1-Atpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Bovine Mitochondrial F1-Atpase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg601 b:36.2 occ:1.00 O2G B:ANP600 2.1 37.5 1.0 OG1 B:THR176 2.1 32.0 1.0 O2B B:ANP600 2.3 35.4 1.0 O B:HOH627 3.0 53.7 1.0 CB B:THR176 3.3 28.0 1.0 PG B:ANP600 3.4 37.9 1.0 OD1 B:ASP269 3.6 31.3 1.0 PB B:ANP600 3.6 35.1 1.0 N3B B:ANP600 3.9 36.1 1.0 N B:THR176 3.9 27.0 1.0 OD2 B:ASP269 4.1 27.8 1.0 CA B:THR176 4.2 27.7 1.0 CG B:ASP269 4.3 28.8 1.0 O1G B:ANP600 4.3 38.9 1.0 O3G B:ANP600 4.3 38.6 1.0 O B:HOH635 4.3 29.1 1.0 CG2 B:THR176 4.4 27.9 1.0 O1B B:ANP600 4.6 34.7 1.0 OE1 B:GLN208 4.6 48.0 1.0 O3A B:ANP600 4.7 34.3 1.0 O B:HOH657 4.8 47.0 1.0 NZ B:LYS175 4.9 21.7 1.0 O B:HOH655 4.9 23.9 1.0 O2A B:ANP600 4.9 30.4 1.0 CB B:LYS175 5.0 25.5 1.0

Magnesium binding site 3 out of 5 in the Bovine Mitochondrial F1-Atpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Bovine Mitochondrial F1-Atpase within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg601 b:25.5 occ:1.00 O2G C:ANP600 2.1 26.2 1.0 O2B C:ANP600 2.2 20.9 1.0 OG1 C:THR176 2.2 29.5 1.0 CB C:THR176 3.3 22.9 1.0 PB C:ANP600 3.4 21.4 1.0 PG C:ANP600 3.5 24.6 1.0 N3B C:ANP600 3.7 23.0 1.0 O2A C:ANP600 4.0 19.5 1.0 N C:THR176 4.0 21.4 1.0 O3G C:ANP600 4.0 24.5 1.0 O1B C:ANP600 4.2 19.9 1.0 CA C:THR176 4.2 21.3 1.0 OD2 C:ASP269 4.2 19.2 1.0 CG2 C:THR176 4.3 21.4 1.0 OD1 C:ASP269 4.4 22.8 1.0 O1G C:ANP600 4.4 25.0 1.0 O3A C:ANP600 4.6 20.4 1.0 O C:HOH635 4.7 30.7 1.0 CG C:ASP269 4.8 20.1 1.0 PA C:ANP600 4.8 19.6 1.0 O1A C:ANP600 4.8 21.2 1.0 NZ C:LYS175 5.0 29.1 1.0

Magnesium binding site 4 out of 5 in the Bovine Mitochondrial F1-Atpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Bovine Mitochondrial F1-Atpase within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg601 b:22.9 occ:1.00 OG1 D:THR163 2.2 26.8 1.0 O2B D:ADP600 2.3 24.9 1.0 CB D:THR163 3.3 23.1 1.0 PB D:ADP600 3.6 25.1 1.0 OE1 D:GLU188 3.9 23.5 1.0 O D:HOH663 3.9 31.1 1.0 O3B D:ADP600 3.9 24.8 1.0 NH1 D:ARG189 4.1 20.0 1.0 N D:THR163 4.1 19.5 1.0 OD1 D:ASP256 4.1 25.4 1.0 OE1 D:GLU192 4.2 28.9 1.0 OD2 D:ASP256 4.3 26.4 1.0 CA D:THR163 4.3 21.1 1.0 CG2 D:THR163 4.4 22.7 1.0 OE2 D:GLU192 4.4 28.7 1.0 O3A D:ADP600 4.4 24.2 1.0 CD D:GLU188 4.5 24.6 1.0 O C:HOH679 4.6 25.8 1.0 CG D:ASP256 4.6 24.4 1.0 O1B D:ADP600 4.7 24.2 1.0 O2A D:ADP600 4.7 21.0 1.0 NH1 C:ARG373 4.7 28.7 1.0 CD D:GLU192 4.7 28.7 1.0 CE D:LYS162 4.7 7.4 1.0 O D:HOH604 4.8 15.8 1.0 O1A D:ADP600 4.9 21.2 1.0 PA D:ADP600 4.9 22.1 1.0 CB D:LYS162 4.9 14.2 1.0 CG D:GLU188 5.0 23.0 1.0

Magnesium binding site 5 out of 5 in the Bovine Mitochondrial F1-Atpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Bovine Mitochondrial F1-Atpase within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg601 b:24.4 occ:1.00 O2G F:ANP600 2.2 29.4 1.0 OG1 F:THR163 2.2 21.1 1.0 O2B F:ANP600 2.4 29.4 1.0 CB F:THR163 3.4 17.1 1.0 PG F:ANP600 3.5 29.4 1.0 OE1 F:GLU188 3.6 30.0 1.0 PB F:ANP600 3.7 29.6 1.0 N3B F:ANP600 3.8 29.1 1.0 OD1 F:ASP256 3.9 25.7 1.0 NH1 F:ARG189 4.0 26.7 1.0 OE2 F:GLU192 4.0 32.2 1.0 O F:HOH625 4.0 34.6 1.0 OD2 F:ASP256 4.1 24.8 1.0 N F:THR163 4.2 19.4 1.0 OE1 F:GLU192 4.3 32.8 1.0 CA F:THR163 4.3 19.4 1.0 O3G F:ANP600 4.4 28.3 1.0 O2A F:ANP600 4.4 27.5 1.0 CG2 F:THR163 4.4 15.2 1.0 CD F:GLU188 4.5 29.9 1.0 O1G F:ANP600 4.5 29.1 1.0 CG F:ASP256 4.5 25.0 1.0 O1B F:ANP600 4.5 28.9 1.0 CD F:GLU192 4.6 30.1 1.0 CE F:LYS162 4.7 13.7 1.0 O3A F:ANP600 4.8 27.8 1.0 O B:HOH612 4.9 18.9 1.0

J.P.Abrahams, A.G.Leslie, R.Lutter, J.E.Walker. Structure at 2.8 A Resolution of F1-Atpase From Bovine Heart Mitochondria. Nature V. 370 621 1994.
ISSN: ISSN 0028-0836
PubMed: 8065448
DOI: 10.1038/370621A0