Magnesium in PDB 1bof: Gi-Alpha-1 Bound to Gdp and Magnesium

The structure of Gi-Alpha-1 Bound to Gdp and Magnesium, PDB code: 1bof was solved by D.E.Coleman, S.R.Sprang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.20
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	121.747, 121.747, 68.929, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 26.8

The binding sites of Magnesium atom in the Gi-Alpha-1 Bound to Gdp and Magnesium (pdb code 1bof). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Gi-Alpha-1 Bound to Gdp and Magnesium, PDB code: 1bof:

Magnesium binding site 1 out of 1 in the Gi-Alpha-1 Bound to Gdp and Magnesium


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Gi-Alpha-1 Bound to Gdp and Magnesium within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg800 b:35.7 occ:1.00 OG1 A:THR181 2.1 50.2 1.0 O A:HOH802 2.1 27.8 1.0 OG A:SER47 2.2 29.7 1.0 O3B A:GDP355 2.2 21.3 1.0 O A:HOH801 2.4 33.6 1.0 O A:HOH803 2.5 48.4 1.0 CB A:SER47 3.2 25.7 1.0 CB A:THR181 3.2 45.3 1.0 PB A:GDP355 3.4 23.5 1.0 O1B A:GDP355 3.6 22.8 1.0 N A:THR181 4.0 43.1 1.0 N A:SER47 4.1 22.3 1.0 OD2 A:ASP200 4.1 39.4 1.0 CA A:SER47 4.2 20.4 1.0 CG2 A:THR181 4.2 45.9 1.0 O A:HOH443 4.2 59.6 1.0 CA A:THR181 4.2 45.6 1.0 O1A A:GDP355 4.3 24.3 1.0 NH1 A:ARG178 4.3 23.1 1.0 O3A A:GDP355 4.4 23.6 1.0 O A:VAL179 4.4 36.7 1.0 O1 A:SO4804 4.5 52.6 1.0 O2B A:GDP355 4.5 21.3 1.0 O A:HOH442 4.5 53.4 1.0 PA A:GDP355 4.7 22.2 1.0 OD1 A:ASP200 4.8 37.5 1.0 CG A:ASP200 4.9 38.3 1.0 O2A A:GDP355 4.9 21.8 1.0 O3 A:SO4804 4.9 51.9 1.0 C A:LYS180 5.0 40.9 1.0

D.E.Coleman, S.R.Sprang. Crystal Structures of the G Protein Gi Alpha 1 Complexed with Gdp and MG2+: A Crystallographic Titration Experiment. Biochemistry V. 37 14376 1998.
ISSN: ISSN 0006-2960
PubMed: 9772163
DOI: 10.1021/BI9810306