Magnesium in PDB 1cm8: Phosphorylated Map Kinase P38-Gamma

The structure of Phosphorylated Map Kinase P38-Gamma, PDB code: 1cm8 was solved by S.Bellon, M.J.Fitzgibbon, T.Fox, H.M.Hsiao, K.P.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	63.500, 66.820, 206.020, 90.00, 90.00, 90.00
R / Rfree (%)	23.2 / 28.3

The binding sites of Magnesium atom in the Phosphorylated Map Kinase P38-Gamma (pdb code 1cm8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Phosphorylated Map Kinase P38-Gamma, PDB code: 1cm8:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Phosphorylated Map Kinase P38-Gamma


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Phosphorylated Map Kinase P38-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:39.3 occ:1.00 O2G A:ANP400 2.1 64.3 1.0 OD1 A:ASN158 2.1 37.2 1.0 O2A A:ANP400 2.3 39.7 1.0 N3B A:ANP400 2.5 55.7 1.0 PG A:ANP400 2.8 66.7 1.0 O A:HOH2039 2.9 59.3 1.0 OD2 A:ASP171 3.0 39.8 1.0 CG A:ASN158 3.3 43.2 1.0 PA A:ANP400 3.5 45.1 1.0 C5' A:ANP400 3.8 40.8 1.0 O A:GLY157 3.8 39.6 1.0 O1G A:ANP400 3.8 65.3 1.0 PB A:ANP400 3.8 53.0 1.0 CG A:ASP171 3.9 44.8 1.0 O5' A:ANP400 4.0 44.3 1.0 ND2 A:ASN158 4.0 34.9 1.0 O3G A:ANP400 4.1 61.8 1.0 O3A A:ANP400 4.1 46.4 1.0 CB A:ASP171 4.2 42.7 1.0 CE A:LYS155 4.2 44.1 1.0 C A:GLY157 4.2 37.1 1.0 CB A:ASN158 4.4 40.1 1.0 CA A:ASN158 4.4 38.0 1.0 N A:ASN158 4.5 35.9 1.0 O3' A:ANP400 4.7 51.0 1.0 O2B A:ANP400 4.7 44.5 1.0 C3' A:ANP400 4.7 44.4 1.0 O1A A:ANP400 4.8 43.3 1.0 NZ A:LYS155 4.8 48.4 1.0 O1B A:ANP400 4.8 55.0 1.0 C4' A:ANP400 4.8 40.7 1.0

Magnesium binding site 2 out of 4 in the Phosphorylated Map Kinase P38-Gamma


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Phosphorylated Map Kinase P38-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:42.8 occ:1.00 OD1 A:ASP171 2.3 47.1 1.0 O3G A:ANP400 2.5 61.8 1.0 O2B A:ANP400 2.6 44.5 1.0 O A:HOH2153 2.7 48.9 1.0 O A:HOH2152 2.7 45.1 1.0 OD2 A:ASP171 2.8 39.8 1.0 CG A:ASP171 2.9 44.8 1.0 PG A:ANP400 3.8 66.7 1.0 CA A:GLY173 3.8 46.8 1.0 PB A:ANP400 3.9 53.0 1.0 N3B A:ANP400 4.1 55.7 1.0 N A:GLY173 4.2 45.2 1.0 NZ A:LYS56 4.3 41.3 1.0 C A:GLY173 4.4 47.4 1.0 CB A:ASP171 4.4 42.7 1.0 O2G A:ANP400 4.5 64.3 1.0 O A:HOH2111 4.6 58.9 1.0 N A:LEU174 4.8 45.5 1.0 O3A A:ANP400 4.8 46.4 1.0 O1G A:ANP400 5.0 65.3 1.0 O A:GLY173 5.0 49.5 1.0

Magnesium binding site 3 out of 4 in the Phosphorylated Map Kinase P38-Gamma


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Phosphorylated Map Kinase P38-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1401 b:41.3 occ:1.00 O2G B:ANP1400 2.1 64.1 1.0 OD1 B:ASN1158 2.1 35.2 1.0 O2A B:ANP1400 2.3 44.7 1.0 N3B B:ANP1400 2.6 57.4 1.0 PG B:ANP1400 2.8 63.6 1.0 OD2 B:ASP1171 3.0 36.4 1.0 CG B:ASN1158 3.3 40.5 1.0 PA B:ANP1400 3.6 46.4 1.0 O B:GLY1157 3.7 39.7 1.0 C5' B:ANP1400 3.7 40.8 1.0 O1G B:ANP1400 3.8 65.6 1.0 O B:HOH2185 3.8 53.3 1.0 PB B:ANP1400 3.9 56.7 1.0 O5' B:ANP1400 4.0 45.8 1.0 ND2 B:ASN1158 4.0 38.6 1.0 CG B:ASP1171 4.0 45.7 1.0 O3G B:ANP1400 4.1 61.2 1.0 CE B:LYS1155 4.1 43.9 1.0 C B:GLY1157 4.2 36.8 1.0 O3A B:ANP1400 4.2 48.9 1.0 CB B:ASP1171 4.2 42.5 1.0 CB B:ASN1158 4.4 38.4 1.0 CA B:ASN1158 4.5 37.8 1.0 N B:ASN1158 4.5 33.8 1.0 O3' B:ANP1400 4.6 46.6 1.0 C3' B:ANP1400 4.7 41.2 1.0 NZ B:LYS1155 4.8 47.0 1.0 O2B B:ANP1400 4.8 48.8 1.0 O1A B:ANP1400 4.8 41.8 1.0 C4' B:ANP1400 4.8 40.9 1.0 O1B B:ANP1400 4.9 55.9 1.0 CA B:GLY1157 5.0 37.5 1.0

Magnesium binding site 4 out of 4 in the Phosphorylated Map Kinase P38-Gamma


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Phosphorylated Map Kinase P38-Gamma within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1402 b:48.9 occ:1.00 OD1 B:ASP1171 2.3 45.0 1.0 O3G B:ANP1400 2.5 61.2 1.0 O B:HOH2118 2.6 50.7 1.0 O2B B:ANP1400 2.6 48.8 1.0 OD2 B:ASP1171 2.8 36.4 1.0 CG B:ASP1171 2.9 45.7 1.0 O B:HOH2016 3.5 39.0 1.0 PG B:ANP1400 3.7 63.6 1.0 CA B:GLY1173 3.8 47.2 1.0 PB B:ANP1400 3.9 56.7 1.0 N3B B:ANP1400 4.0 57.4 1.0 N B:GLY1173 4.2 44.6 1.0 NZ B:LYS1056 4.3 41.7 1.0 CB B:ASP1171 4.4 42.5 1.0 C B:GLY1173 4.4 49.0 1.0 O2G B:ANP1400 4.4 64.1 1.0 O B:HOH2037 4.5 64.4 1.0 N B:LEU1174 4.8 47.5 1.0 O3A B:ANP1400 4.8 48.9 1.0 O1G B:ANP1400 4.9 65.6 1.0 O B:GLY1173 5.0 50.1 1.0

S.Bellon, M.J.Fitzgibbon, T.Fox, H.M.Hsiao, K.P.Wilson. The Structure of Phosphorylated P38GAMMA Is Monomeric and Reveals A Conserved Activation-Loop Conformation. Structure Fold.Des. V. 7 1057 1999.
ISSN: ISSN 0969-2126
PubMed: 10508788
DOI: 10.1016/S0969-2126(99)80173-7