Magnesium in PDB 1cz7: The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations

The structure of The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations, PDB code: 1cz7 was solved by F.K.Kozielski, S.De Bonis, W.Burmeister, C.Cohen-Addad, R.Wade, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	116.194, 148.827, 261.516, 90.00, 90.00, 90.00
R / Rfree (%)	25.8 / 29.4

The binding sites of Magnesium atom in the The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations (pdb code 1cz7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations, PDB code: 1cz7:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg804 b:47.9 occ:1.00 OG1 A:THR441 2.1 38.7 1.0 O3B A:ADP800 2.7 0.4 1.0 O3A A:ADP800 3.0 79.3 1.0 PB A:ADP800 3.3 0.2 1.0 O1B A:ADP800 3.4 0.2 1.0 CB A:THR441 3.4 47.8 1.0 PA A:ADP800 4.2 83.4 1.0 N A:THR441 4.3 52.2 1.0 CG2 A:THR441 4.3 49.6 1.0 CA A:THR441 4.4 44.7 1.0 O2A A:ADP800 4.4 89.5 1.0 OD1 A:ASP580 4.6 95.2 1.0 O5' A:ADP800 4.7 77.1 1.0 O2B A:ADP800 4.7 0.7 1.0 OD2 A:ASP580 4.7 83.5 1.0

Magnesium binding site 2 out of 4 in the The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg805 b:77.8 occ:1.00 OG1 B:THR441 2.6 90.0 1.0 O3B B:ADP801 2.7 0.9 1.0 PB B:ADP801 3.5 0.5 1.0 O1B B:ADP801 3.5 0.3 1.0 O3A B:ADP801 3.7 0.1 1.0 CB B:THR441 3.9 93.4 1.0 N B:THR441 4.3 89.8 1.0 OD1 B:ASP580 4.5 0.9 1.0 CA B:THR441 4.7 92.3 1.0 CB B:LYS440 4.8 86.1 1.0 O B:LEU581 4.9 0.4 1.0 CE B:LYS440 4.9 97.1 1.0 CG2 B:THR441 4.9 89.7 1.0 O2B B:ADP801 4.9 0.1 1.0 CA B:SER551 5.0 0.6 1.0

Magnesium binding site 3 out of 4 in the The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg806 b:89.7 occ:1.00 O C:HOH37 2.4 74.7 1.0 O3B C:ADP802 2.4 0.1 1.0 OG1 C:THR441 2.5 92.9 1.0 O3A C:ADP802 3.1 0.3 1.0 PB C:ADP802 3.2 0.3 1.0 O1B C:ADP802 3.5 0.6 1.0 CB C:THR441 3.7 96.4 1.0 OD1 C:ASP580 4.1 0.1 1.0 OD2 C:ASP580 4.3 0.5 1.0 N C:THR441 4.3 0.2 1.0 PA C:ADP802 4.5 0.2 1.0 CA C:THR441 4.5 96.7 1.0 O2A C:ADP802 4.6 0.4 1.0 O2B C:ADP802 4.6 0.1 1.0 CG C:ASP580 4.6 0.5 1.0 CG2 C:THR441 4.7 90.0 1.0 CB C:LYS440 5.0 0.6 1.0

Magnesium binding site 4 out of 4 in the The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of The Crystal Structure of A Minus-End Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg807 b:66.3 occ:1.00 O3B D:ADP803 2.3 0.2 1.0 OG1 D:THR441 2.5 35.2 1.0 PB D:ADP803 3.1 99.4 1.0 O3A D:ADP803 3.1 64.9 1.0 O1B D:ADP803 3.3 0.3 1.0 CB D:THR441 3.8 50.2 1.0 O D:HOH152 4.2 74.4 1.0 OD1 D:ASP580 4.2 85.5 1.0 OD2 D:ASP580 4.4 93.8 1.0 N D:THR441 4.4 39.8 1.0 O2B D:ADP803 4.5 84.0 1.0 PA D:ADP803 4.5 66.3 1.0 CE D:LYS440 4.7 54.7 1.0 O2A D:ADP803 4.7 76.4 1.0 CA D:THR441 4.7 44.2 1.0 CG D:ASP580 4.8 83.3 1.0 CG2 D:THR441 4.8 36.7 1.0 CB D:LYS440 5.0 46.3 1.0

F.Kozielski, S.De Bonis, W.P.Burmeister, C.Cohen-Addad, R.H.Wade. The Crystal Structure of the Minus-End-Directed Microtubule Motor Protein Ncd Reveals Variable Dimer Conformations. Structure Fold.Des. V. 7 1407 1999.
ISSN: ISSN 0969-2126
PubMed: 10574799
DOI: 10.1016/S0969-2126(00)80030-1