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Magnesium in PDB 1d9d: Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3'

Enzymatic activity of Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3'

All present enzymatic activity of Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3':
2.7.7.7;

Protein crystallography data

The structure of Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3', PDB code: 1d9d was solved by M.Teplova, S.T.Wallace, V.Tereshko, G.Minasov, A.M.Simons, P.D.Cook, M.Manoharan, M.Egli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.18
Space group P 43
Cell size a, b, c (Å), α, β, γ (°) 102.550, 102.550, 86.280, 90.00, 90.00, 90.00
R / Rfree (%) 21.5 / 23.8

Other elements in 1d9d:

The structure of Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3' also contains other interesting chemical elements:

Zinc (Zn) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3' (pdb code 1d9d). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3', PDB code: 1d9d:

Magnesium binding site 1 out of 1 in 1d9d

Go back to Magnesium Binding Sites List in 1d9d
Magnesium binding site 1 out of 1 in the Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3'


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystall Structure of the Complex of Dna Polymerase I Klenow Fragment with Short Dna Fragment Carrying 2'-0- Aminopropyl-Rna Modifications 5'-D(Tcg)-Ap(Auc)-3' within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg321

b:50.9
occ:1.00
OH A:TYR791 2.0 37.6 1.0
OD1 A:ASN761 2.1 37.7 1.0
CZ A:TYR791 2.9 35.0 1.0
CG A:ASN761 3.0 36.8 1.0
CE2 A:TYR791 3.2 34.9 1.0
CD2 A:LEU723 3.3 23.4 1.0
ND2 A:ASN761 3.5 34.0 1.0
OG1 A:THR737 3.6 35.8 1.0
CD1 A:LEU723 3.8 22.8 1.0
CE A:MET714 4.0 30.3 1.0
CE1 A:TYR791 4.2 34.3 1.0
CG A:LEU723 4.2 24.5 1.0
CB A:ASN761 4.2 35.0 1.0
CG2 A:THR737 4.3 33.9 1.0
CD2 A:TYR791 4.5 32.3 1.0
CB A:THR737 4.6 32.5 1.0
CA A:ASN761 4.6 36.8 1.0
CD1 A:LEU764 4.8 36.9 1.0
CD2 A:TYR795 5.0 26.4 1.0

Reference:

M.Teplova, S.T.Wallace, V.Tereshko, G.Minasov, A.M.Symons, P.D.Cook, M.Manoharan, M.Egli. Structural Origins of the Exonuclease Resistance of A Zwitterionic Rna. Proc.Natl.Acad.Sci.Usa V. 96 14240 1999.
ISSN: ISSN 0027-8424
PubMed: 10588690
DOI: 10.1073/PNAS.96.25.14240
Page generated: Tue Aug 13 02:37:03 2024

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