Magnesium in PDB 1dqn: Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue

All present enzymatic activity of Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue:
2.4.2.8;

The structure of Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue, PDB code: 1dqn was solved by W.Shi, N.R.Munagala, C.C.Wang, C.M.Li, P.C.Tyler, R.H.Furneaux, C.Grubmeyer, V.L.Schramm, S.C.Almo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.632, 71.553, 123.080, 90.00, 90.00, 90.00
R / Rfree (%)	20.4 / 23.4

The binding sites of Magnesium atom in the Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue (pdb code 1dqn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue, PDB code: 1dqn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg450 b:26.4 occ:1.00 O3' A:IMU300 2.0 23.0 1.0 OE2 A:GLU126 2.0 19.6 1.0 OD2 A:ASP125 2.0 22.3 1.0 O A:HOH800 2.4 23.7 1.0 O2' A:IMU300 2.5 24.9 1.0 O A:HOH776 2.6 31.9 1.0 CG A:ASP125 3.0 20.7 1.0 CD A:GLU126 3.0 20.0 1.0 C3' A:IMU300 3.1 22.1 1.0 OE1 A:GLU126 3.2 23.2 1.0 C2' A:IMU300 3.2 22.4 1.0 OD1 A:ASP125 3.2 20.2 1.0 C4' A:IMU300 3.8 21.6 1.0 O2 A:POP400 3.8 44.5 1.0 O A:HOH760 4.1 29.4 1.0 O A:TYR127 4.2 12.2 1.0 N A:GLU126 4.3 13.7 1.0 CB A:ASP125 4.3 18.4 1.0 CG A:GLU126 4.4 16.6 1.0 N A:TYR127 4.4 11.7 1.0 C5' A:IMU300 4.7 21.6 1.0 C1' A:IMU300 4.7 21.6 1.0 O A:POP400 4.7 43.7 1.0 CA A:ASP125 4.8 15.8 1.0 N4' A:IMU300 4.8 21.9 1.0 O A:HOH588 4.9 15.9 1.0 CD2 A:LEU186 4.9 13.9 1.0 C A:ASP125 5.0 14.3 1.0 N A:ALA72 5.0 9.6 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Giardia Guanine Phosphoribosyltransferase Complexed with A Transition State Analogue within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg451 b:31.9 occ:1.00 O A:HOH853 1.9 28.7 1.0 O4 A:POP400 2.0 42.5 1.0 O A:HOH558 2.1 20.5 1.0 OD1 A:ASP187 2.2 15.1 1.0 O A:HOH849 2.3 29.1 1.0 O3 A:POP400 2.4 44.3 1.0 P2 A:POP400 3.2 43.8 1.0 CG A:ASP187 3.3 16.2 1.0 P1 A:POP400 3.5 44.3 1.0 O6 A:POP400 3.6 43.7 1.0 O A:POP400 3.6 43.7 1.0 OD2 A:ASP187 3.8 17.8 1.0 N3 A:IMU300 4.2 15.8 1.0 NH2 A:ARG193 4.3 12.9 1.0 O A:HOH543 4.4 32.5 1.0 O5 A:POP400 4.4 43.9 1.0 O1 A:POP400 4.5 43.8 1.0 NH1 A:ARG193 4.5 11.4 1.0 CB A:ASP187 4.5 14.1 1.0 N A:ASP187 4.6 11.3 1.0 N2 A:IMU300 4.6 12.7 1.0 O2 A:POP400 4.6 44.5 1.0 O2' A:IMU300 4.6 24.9 1.0 C1' A:IMU300 4.8 21.6 1.0 C2 A:IMU300 4.9 14.2 1.0 CZ A:ARG193 4.9 11.9 1.0 O A:ASP187 5.0 12.1 1.0

W.Shi, N.R.Munagala, C.C.Wang, C.M.Li, P.C.Tyler, R.H.Furneaux, C.Grubmeyer, V.L.Schramm, S.C.Almo. Crystal Structures of Giardia Lamblia Guanine Phosphoribosyltransferase at 1.75 A(,). Biochemistry V. 39 6781 2000.
ISSN: ISSN 0006-2960
PubMed: 10841757
DOI: 10.1021/BI000128T