Magnesium in PDB 1dxe: 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli

All present enzymatic activity of 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli:
4.1.2.20;

The structure of 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli, PDB code: 1dxe was solved by T.Izard, N.C.Blackwell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20 / 1.8
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	129.070, 129.070, 167.960, 90.00, 90.00, 120.00
R / Rfree (%)	17.1 / 19.4

The binding sites of Magnesium atom in the 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli (pdb code 1dxe). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli, PDB code: 1dxe:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg901 b:17.1 occ:1.00 O A:HOH2081 2.2 11.2 1.0 OD2 A:ASP179 2.2 14.7 1.0 OE1 A:GLU153 2.2 14.5 1.0 O1 A:PO4301 2.3 23.6 1.0 O A:HOH2326 2.4 16.1 1.0 CD A:GLU153 3.1 15.2 1.0 CG A:ASP179 3.2 14.7 1.0 P A:PO4301 3.4 24.3 1.0 OE2 A:GLU153 3.4 14.9 1.0 O4 A:PO4301 3.6 24.0 1.0 CB A:ASP179 3.7 14.4 1.0 O A:HOH2087 3.8 15.9 1.0 OE1 A:GLN151 3.8 16.1 1.0 O3 A:PO4301 3.8 23.5 1.0 NH1 A:ARG75 4.0 14.8 1.0 OE1 A:GLU49 4.3 13.2 1.0 OD1 A:ASP179 4.3 14.3 1.0 CG A:GLU153 4.4 14.9 1.0 CA A:GLY176 4.5 15.3 1.0 N A:ASP179 4.5 14.3 1.0 O2 A:PO4301 4.6 23.9 1.0 CD A:GLN151 4.7 14.7 1.0 CB A:GLU153 4.7 15.5 1.0 NE2 A:HIS50 4.7 12.3 1.0 CA A:ASP179 4.8 14.5 1.0 NE2 A:GLN151 4.8 14.1 1.0 CZ A:ARG75 4.9 14.1 1.0 O A:GLY176 4.9 15.0 1.0 C A:GLY176 5.0 15.4 1.0 CD2 A:HIS50 5.0 13.1 1.0

Magnesium binding site 2 out of 2 in the 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 2-Dehydro-3-Deoxy-Galactarate Aldolase From Escherichia Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg901 b:15.9 occ:1.00 OD2 B:ASP179 2.2 12.4 1.0 O B:HOH2086 2.2 11.7 1.0 OE1 B:GLU153 2.2 13.1 1.0 O3 B:PO4301 2.2 19.8 1.0 O B:HOH2322 2.4 15.6 1.0 CD B:GLU153 3.1 13.6 1.0 CG B:ASP179 3.2 12.9 1.0 P B:PO4301 3.3 20.6 1.0 OE2 B:GLU153 3.4 13.3 1.0 O2 B:PO4301 3.6 20.0 1.0 CB B:ASP179 3.7 12.4 1.0 O1 B:PO4301 3.8 19.9 1.0 O B:HOH2091 3.8 12.7 1.0 OE1 B:GLN151 3.9 15.5 1.0 NH1 B:ARG75 4.0 14.6 1.0 OD1 B:ASP179 4.3 12.7 1.0 OE1 B:GLU49 4.4 12.6 1.0 CG B:GLU153 4.5 13.5 1.0 N B:ASP179 4.5 12.8 1.0 CA B:GLY176 4.5 14.7 1.0 O4 B:PO4301 4.6 20.4 1.0 CA B:ASP179 4.7 12.8 1.0 CD B:GLN151 4.7 14.2 1.0 CB B:GLU153 4.7 14.2 1.0 NE2 B:HIS50 4.8 11.2 1.0 CZ B:ARG75 4.8 13.4 1.0 NE2 B:GLN151 4.9 13.3 1.0 O B:GLY176 4.9 14.4 1.0 C B:GLY176 5.0 14.8 1.0

T.Izard, N.C.Blackwell. Crystal Structures of the Metal-Dependent 2-Dehydro-3-Deoxy-Galactarate Aldolase Suggest A Novel Reaction Mechanism. Embo J. V. 19 3849 2000.
ISSN: ISSN 0261-4189
PubMed: 10921867
DOI: 10.1093/EMBOJ/19.15.3849