Magnesium in PDB 1e0j: GP4D Helicase From Phage T7 Adpnp Complex

The structure of GP4D Helicase From Phage T7 Adpnp Complex, PDB code: 1e0j was solved by M.R.Singleton, M.R.Sawaya, T.Ellenberger, D.B.Wigley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.0 / 3.0
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	119.180, 119.180, 283.420, 90.00, 90.00, 90.00
R / Rfree (%)	25.3 / 30.4

The binding sites of Magnesium atom in the GP4D Helicase From Phage T7 Adpnp Complex (pdb code 1e0j). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the GP4D Helicase From Phage T7 Adpnp Complex, PDB code: 1e0j:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the GP4D Helicase From Phage T7 Adpnp Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of GP4D Helicase From Phage T7 Adpnp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg701 b:38.5 occ:1.00 O1B A:ANP700 2.1 49.6 1.0 O2G A:ANP700 2.4 45.5 1.0 OG A:SER319 2.7 55.7 1.0 PB A:ANP700 3.1 51.6 1.0 N3B A:ANP700 3.1 50.0 1.0 PG A:ANP700 3.3 49.2 1.0 OE1 A:GLU343 3.7 84.0 1.0 OD2 A:ASP424 3.8 63.0 1.0 CB A:SER319 3.9 57.8 1.0 O3A A:ANP700 4.1 46.8 1.0 OD1 A:ASP424 4.2 60.7 1.0 O1G A:ANP700 4.2 55.4 1.0 O2B A:ANP700 4.2 44.5 1.0 CG A:ASP424 4.4 60.8 1.0 O3G A:ANP700 4.5 47.9 1.0 O2A A:ANP700 4.7 50.4 1.0 PA A:ANP700 4.9 46.9 1.0 CD A:GLU343 4.9 81.2 1.0 N A:SER319 5.0 62.4 1.0

Magnesium binding site 2 out of 4 in the GP4D Helicase From Phage T7 Adpnp Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of GP4D Helicase From Phage T7 Adpnp Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg701 b:39.0 occ:1.00 OG B:SER319 2.1 46.6 1.0 O1B B:ANP700 2.3 48.4 1.0 O2G B:ANP700 2.5 45.9 1.0 PB B:ANP700 3.3 53.7 1.0 N3B B:ANP700 3.3 49.7 1.0 CB B:SER319 3.3 48.0 1.0 PG B:ANP700 3.5 46.8 1.0 OD2 B:ASP424 3.6 55.0 1.0 OD1 B:ASP424 4.0 50.7 1.0 OE1 B:GLU343 4.0 65.3 1.0 CG B:ASP424 4.2 52.9 1.0 O3A B:ANP700 4.3 45.0 1.0 O1G B:ANP700 4.3 57.9 1.0 O2B B:ANP700 4.4 46.6 1.0 NH2 C:ARG522 4.5 87.2 1.0 CA B:SER319 4.5 47.7 1.0 N B:SER319 4.5 49.7 1.0 O3G B:ANP700 4.6 46.5 1.0 O2A B:ANP700 4.9 43.8 1.0

Magnesium binding site 3 out of 4 in the GP4D Helicase From Phage T7 Adpnp Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of GP4D Helicase From Phage T7 Adpnp Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg701 b:43.3 occ:1.00 O1B D:ANP700 2.0 52.3 1.0 O2G D:ANP700 2.2 48.8 1.0 OG D:SER319 2.6 50.3 1.0 PB D:ANP700 2.9 56.5 1.0 N3B D:ANP700 3.0 52.7 1.0 PG D:ANP700 3.1 52.8 1.0 CB D:SER319 3.7 54.3 1.0 O1G D:ANP700 3.9 59.5 1.0 O2B D:ANP700 4.0 46.7 1.0 O3A D:ANP700 4.1 46.2 1.0 OD2 D:ASP424 4.1 58.7 1.0 OD1 D:ASP424 4.2 54.3 1.0 OE1 D:GLU343 4.3 77.8 1.0 O3G D:ANP700 4.4 48.9 1.0 N D:SER319 4.4 59.4 1.0 CG D:ASP424 4.6 56.0 1.0 CA D:SER319 4.7 57.1 1.0 CE D:LYS318 4.7 63.2 1.0 O2A D:ANP700 4.8 50.7 1.0 NZ D:LYS318 4.9 62.8 1.0 PA D:ANP700 4.9 45.9 1.0

Magnesium binding site 4 out of 4 in the GP4D Helicase From Phage T7 Adpnp Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of GP4D Helicase From Phage T7 Adpnp Complex within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg701 b:43.8 occ:1.00 OG E:SER319 2.4 51.3 1.0 O2G E:ANP700 2.6 50.9 1.0 O1B E:ANP700 2.7 50.3 1.0 OD2 E:ASP424 3.6 54.8 1.0 N3B E:ANP700 3.6 52.1 1.0 CB E:SER319 3.6 49.4 1.0 PB E:ANP700 3.7 53.4 1.0 OE1 E:GLU343 3.7 70.8 1.0 PG E:ANP700 3.7 55.4 1.0 OD1 E:ASP424 4.0 53.1 1.0 CG E:ASP424 4.2 54.7 1.0 NH2 F:ARG522 4.5 93.3 1.0 O3A E:ANP700 4.6 43.3 1.0 O1G E:ANP700 4.7 55.8 1.0 CA E:SER319 4.7 50.0 1.0 O3G E:ANP700 4.7 51.8 1.0 N E:SER319 4.8 51.1 1.0 O2B E:ANP700 4.9 45.6 1.0 CD E:GLU343 4.9 68.7 1.0 O2A E:ANP700 5.0 47.4 1.0

M.R.Singleton, M.R.Sawaya, T.Ellenberger, D.B.Wigley. Crystal Structure of T7 Gene 4 Ring Helicase Indicates A Mechanism For Sequential Hydrolysis of Nucleotides Cell(Cambridge,Mass.) V. 101 589 2000.
ISSN: ISSN 0092-8674
PubMed: 10892646
DOI: 10.1016/S0092-8674(00)80871-5