Magnesium in PDB 1e1q: Bovine Mitochondrial F1-Atpase at 100K
Enzymatic activity of Bovine Mitochondrial F1-Atpase at 100K
All present enzymatic activity of Bovine Mitochondrial F1-Atpase at 100K:
3.6.1.34;
Protein crystallography data
The structure of Bovine Mitochondrial F1-Atpase at 100K, PDB code: 1e1q
was solved by
K.Braig,
R.I.Menz,
M.G.Montgomery,
A.G.W.Leslie,
J.E.Walker,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
20 /
2.61
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
280.800,
107.400,
139.600,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
23.2 /
28
|
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Bovine Mitochondrial F1-Atpase at 100K
(pdb code 1e1q). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the
Bovine Mitochondrial F1-Atpase at 100K, PDB code: 1e1q:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
Magnesium binding site 1 out
of 5 in 1e1q
Go back to
Magnesium Binding Sites List in 1e1q
Magnesium binding site 1 out
of 5 in the Bovine Mitochondrial F1-Atpase at 100K
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Bovine Mitochondrial F1-Atpase at 100K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg601
b:49.8
occ:1.00
|
O2G
|
A:ANP600
|
2.1
|
43.5
|
1.0
|
OG1
|
A:THR176
|
2.2
|
46.5
|
1.0
|
O
|
A:HOH2043
|
2.2
|
36.4
|
1.0
|
O2B
|
A:ANP600
|
2.4
|
35.3
|
1.0
|
PG
|
A:ANP600
|
3.3
|
44.2
|
1.0
|
CB
|
A:THR176
|
3.4
|
36.0
|
1.0
|
PB
|
A:ANP600
|
3.6
|
36.3
|
1.0
|
N3B
|
A:ANP600
|
3.6
|
41.8
|
1.0
|
O
|
A:HOH2044
|
3.8
|
52.2
|
1.0
|
O3G
|
A:ANP600
|
3.9
|
42.9
|
1.0
|
OD2
|
A:ASP269
|
4.1
|
43.2
|
1.0
|
OD1
|
A:ASP269
|
4.2
|
52.1
|
1.0
|
O
|
A:HOH2045
|
4.2
|
36.9
|
1.0
|
CG2
|
A:THR176
|
4.3
|
37.1
|
1.0
|
N
|
A:THR176
|
4.4
|
42.9
|
1.0
|
O2A
|
A:ANP600
|
4.5
|
42.2
|
1.0
|
O1G
|
A:ANP600
|
4.5
|
45.0
|
1.0
|
CA
|
A:THR176
|
4.5
|
43.2
|
1.0
|
O
|
D:HOH2029
|
4.5
|
52.5
|
1.0
|
CG
|
A:ASP269
|
4.6
|
53.5
|
1.0
|
O1B
|
A:ANP600
|
4.6
|
34.9
|
1.0
|
O3A
|
A:ANP600
|
4.6
|
41.4
|
1.0
|
PA
|
A:ANP600
|
5.0
|
40.8
|
1.0
|
|
Magnesium binding site 2 out
of 5 in 1e1q
Go back to
Magnesium Binding Sites List in 1e1q
Magnesium binding site 2 out
of 5 in the Bovine Mitochondrial F1-Atpase at 100K
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Bovine Mitochondrial F1-Atpase at 100K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg601
b:62.8
occ:1.00
|
OG1
|
B:THR176
|
2.0
|
59.7
|
1.0
|
O2G
|
B:ANP600
|
2.1
|
52.2
|
1.0
|
O2B
|
B:ANP600
|
2.1
|
54.3
|
1.0
|
CB
|
B:THR176
|
2.9
|
50.4
|
1.0
|
PG
|
B:ANP600
|
3.4
|
46.7
|
1.0
|
PB
|
B:ANP600
|
3.5
|
48.7
|
1.0
|
N3B
|
B:ANP600
|
3.8
|
45.1
|
1.0
|
CG2
|
B:THR176
|
3.9
|
47.1
|
1.0
|
N
|
B:THR176
|
3.9
|
44.6
|
1.0
|
OD2
|
B:ASP269
|
4.0
|
47.5
|
1.0
|
CA
|
B:THR176
|
4.0
|
49.3
|
1.0
|
OD1
|
B:ASP269
|
4.1
|
52.9
|
1.0
|
O
|
B:HOH2083
|
4.1
|
56.2
|
1.0
|
O3G
|
B:ANP600
|
4.1
|
45.9
|
1.0
|
O1B
|
B:ANP600
|
4.3
|
50.3
|
1.0
|
O1G
|
B:ANP600
|
4.4
|
51.2
|
1.0
|
O
|
B:HOH2029
|
4.4
|
60.0
|
1.0
|
O2A
|
B:ANP600
|
4.5
|
50.9
|
1.0
|
CG
|
B:ASP269
|
4.5
|
55.1
|
1.0
|
O
|
B:HOH2041
|
4.5
|
41.4
|
1.0
|
O3A
|
B:ANP600
|
4.5
|
52.9
|
1.0
|
NZ
|
B:LYS175
|
4.7
|
44.8
|
1.0
|
O1A
|
B:ANP600
|
4.7
|
48.7
|
1.0
|
PA
|
B:ANP600
|
4.8
|
49.8
|
1.0
|
O
|
B:HOH2024
|
5.0
|
58.9
|
1.0
|
|
Magnesium binding site 3 out
of 5 in 1e1q
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Magnesium Binding Sites List in 1e1q
Magnesium binding site 3 out
of 5 in the Bovine Mitochondrial F1-Atpase at 100K
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Bovine Mitochondrial F1-Atpase at 100K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg601
b:46.2
occ:1.00
|
O
|
C:HOH2035
|
2.0
|
45.5
|
1.0
|
O2B
|
C:ANP600
|
2.2
|
45.7
|
1.0
|
O2G
|
C:ANP600
|
2.2
|
41.7
|
1.0
|
OG1
|
C:THR176
|
2.3
|
45.4
|
1.0
|
CB
|
C:THR176
|
3.1
|
38.0
|
1.0
|
PG
|
C:ANP600
|
3.3
|
41.1
|
1.0
|
PB
|
C:ANP600
|
3.3
|
43.3
|
1.0
|
N3B
|
C:ANP600
|
3.4
|
45.4
|
1.0
|
O
|
C:HOH2041
|
3.9
|
68.2
|
1.0
|
O3G
|
C:ANP600
|
3.9
|
43.2
|
1.0
|
CG2
|
C:THR176
|
4.0
|
37.4
|
1.0
|
O2A
|
C:ANP600
|
4.1
|
42.6
|
1.0
|
N
|
C:THR176
|
4.2
|
40.3
|
1.0
|
CA
|
C:THR176
|
4.3
|
40.5
|
1.0
|
OD2
|
C:ASP269
|
4.3
|
38.6
|
1.0
|
O3A
|
C:ANP600
|
4.3
|
44.4
|
1.0
|
O1B
|
C:ANP600
|
4.4
|
43.0
|
1.0
|
O1G
|
C:ANP600
|
4.4
|
42.1
|
1.0
|
OD1
|
C:ASP269
|
4.4
|
40.9
|
1.0
|
PA
|
C:ANP600
|
4.6
|
44.2
|
1.0
|
O
|
C:HOH2057
|
4.6
|
52.8
|
1.0
|
O1A
|
C:ANP600
|
4.7
|
43.8
|
1.0
|
CG
|
C:ASP269
|
4.8
|
46.3
|
1.0
|
|
Magnesium binding site 4 out
of 5 in 1e1q
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Magnesium Binding Sites List in 1e1q
Magnesium binding site 4 out
of 5 in the Bovine Mitochondrial F1-Atpase at 100K
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Bovine Mitochondrial F1-Atpase at 100K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg601
b:55.6
occ:1.00
|
O
|
D:HOH2037
|
2.2
|
52.6
|
1.0
|
OG1
|
D:THR163
|
2.3
|
43.6
|
1.0
|
O2B
|
D:ADP600
|
2.3
|
41.1
|
1.0
|
CB
|
D:THR163
|
2.9
|
45.7
|
1.0
|
PB
|
D:ADP600
|
3.5
|
39.4
|
1.0
|
O2A
|
D:ADP600
|
3.7
|
39.1
|
1.0
|
OE2
|
D:GLU192
|
3.7
|
55.2
|
1.0
|
CG2
|
D:THR163
|
3.8
|
31.6
|
1.0
|
O3B
|
D:ADP600
|
3.8
|
44.0
|
1.0
|
NH1
|
D:ARG189
|
4.0
|
41.0
|
1.0
|
N
|
D:THR163
|
4.0
|
41.7
|
1.0
|
CA
|
D:THR163
|
4.0
|
42.1
|
1.0
|
O3A
|
D:ADP600
|
4.1
|
40.6
|
1.0
|
OD2
|
D:ASP256
|
4.3
|
41.2
|
1.0
|
OE1
|
D:GLU192
|
4.3
|
62.0
|
1.0
|
PA
|
D:ADP600
|
4.3
|
39.3
|
1.0
|
O1A
|
D:ADP600
|
4.4
|
31.4
|
1.0
|
O
|
D:HOH2038
|
4.4
|
46.1
|
1.0
|
CD
|
D:GLU192
|
4.4
|
59.5
|
1.0
|
NH1
|
C:ARG373
|
4.5
|
51.1
|
1.0
|
OD1
|
D:ASP256
|
4.5
|
51.4
|
1.0
|
O1B
|
D:ADP600
|
4.7
|
39.8
|
1.0
|
O
|
D:HOH2039
|
4.8
|
61.4
|
1.0
|
CG
|
D:ASP256
|
4.9
|
51.4
|
1.0
|
OE1
|
D:GLU188
|
4.9
|
65.9
|
1.0
|
|
Magnesium binding site 5 out
of 5 in 1e1q
Go back to
Magnesium Binding Sites List in 1e1q
Magnesium binding site 5 out
of 5 in the Bovine Mitochondrial F1-Atpase at 100K
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of Bovine Mitochondrial F1-Atpase at 100K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Mg601
b:50.1
occ:1.00
|
O2G
|
F:ANP600
|
2.1
|
44.1
|
1.0
|
O2B
|
F:ANP600
|
2.2
|
50.1
|
1.0
|
OG1
|
F:THR163
|
2.3
|
37.5
|
1.0
|
O
|
F:HOH2107
|
2.3
|
47.1
|
1.0
|
CB
|
F:THR163
|
3.0
|
39.1
|
1.0
|
PB
|
F:ANP600
|
3.4
|
48.5
|
1.0
|
PG
|
F:ANP600
|
3.4
|
46.0
|
1.0
|
N3B
|
F:ANP600
|
3.7
|
47.3
|
1.0
|
OE1
|
F:GLU192
|
3.8
|
63.2
|
1.0
|
CG2
|
F:THR163
|
3.9
|
38.8
|
1.0
|
NH1
|
F:ARG189
|
4.0
|
28.6
|
1.0
|
OE2
|
F:GLU192
|
4.1
|
67.4
|
1.0
|
N
|
F:THR163
|
4.1
|
44.6
|
1.0
|
CA
|
F:THR163
|
4.1
|
39.8
|
1.0
|
O2A
|
F:ANP600
|
4.1
|
46.3
|
1.0
|
OD2
|
F:ASP256
|
4.2
|
36.0
|
1.0
|
OE1
|
F:GLU188
|
4.2
|
62.4
|
1.0
|
O3A
|
F:ANP600
|
4.3
|
44.5
|
1.0
|
O3G
|
F:ANP600
|
4.4
|
42.6
|
1.0
|
CD
|
F:GLU192
|
4.4
|
62.1
|
1.0
|
O1G
|
F:ANP600
|
4.4
|
48.7
|
1.0
|
OD1
|
F:ASP256
|
4.4
|
42.4
|
1.0
|
NH1
|
B:ARG373
|
4.5
|
47.6
|
1.0
|
O1B
|
F:ANP600
|
4.5
|
51.3
|
1.0
|
O
|
F:HOH2054
|
4.6
|
58.8
|
1.0
|
PA
|
F:ANP600
|
4.6
|
42.2
|
1.0
|
O1A
|
F:ANP600
|
4.8
|
44.4
|
1.0
|
CG
|
F:ASP256
|
4.9
|
47.5
|
1.0
|
CE
|
F:LYS162
|
4.9
|
44.9
|
1.0
|
|
Reference:
K.Braig,
R.I.Menz,
M.G.Montgomery,
A.G.W.Leslie,
J.E.Walker.
Structure of Bovine Mitochondrial F1-Atpase Inhibited By MG2+Adp and Aluminium Fluoride Structure V. 8 567 2000.
ISSN: ISSN 0969-2126
PubMed: 10873854
DOI: 10.1016/S0969-2126(00)00145-3
Page generated: Tue Aug 13 02:49:09 2024
|