Magnesium in PDB 1e3m: The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch

The structure of The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch, PDB code: 1e3m was solved by M.H.Lamers, A.Perrakis, J.H.Enzlin, H.H.K.Winterwerp, N.De Wind, T.K.Sixma, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	89.960, 92.370, 261.330, 90.00, 90.00, 90.00
R / Rfree (%)	22.8 / 26.6

The binding sites of Magnesium atom in the The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch (pdb code 1e3m). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch, PDB code: 1e3m:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1001 b:40.3 occ:1.00 O A:HOH2228 2.0 38.1 1.0 O A:HOH2200 2.1 42.5 1.0 O A:HOH2188 2.1 39.0 1.0 O2B A:ADP1801 2.1 41.4 1.0 O A:HOH2227 2.1 41.2 1.0 OG A:SER621 2.1 37.9 1.0 CB A:SER621 2.8 38.9 1.0 PB A:ADP1801 3.3 41.9 1.0 O1B A:ADP1801 3.4 40.6 1.0 OD2 A:ASP693 3.7 42.2 1.0 N A:SER621 3.8 40.4 1.0 O1A A:ADP1801 3.8 43.2 1.0 CA A:SER621 3.8 39.5 1.0 OD1 A:ASP693 4.1 43.7 1.0 O3A A:ADP1801 4.3 40.7 1.0 PA A:ADP1801 4.4 44.3 1.0 CG A:ASP693 4.4 42.4 1.0 O3B A:ADP1801 4.4 41.4 1.0 O2A A:ADP1801 4.5 46.1 1.0 OE2 A:GLU694 4.5 44.3 1.0 C A:LYS620 4.9 41.0 1.0

Magnesium binding site 2 out of 2 in the The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of E. Coli Muts Binding to Dna with A G:T Mismatch within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1011 b:57.7 occ:1.00 O A:HOH2034 2.1 59.0 1.0 O A:HOH2036 2.1 57.5 1.0 O A:HOH2035 2.1 58.6 1.0 O A:HOH2037 2.1 58.2 1.0 O A:SER102 2.1 57.6 1.0 O A:PRO99 2.7 57.4 1.0 C A:SER102 3.3 57.5 1.0 C A:PRO99 3.7 57.3 1.0 O A:LYS103 3.7 55.7 1.0 CA A:SER102 4.0 57.8 1.0 CB A:SER102 4.0 57.8 1.0 O A:GLY104 4.1 49.7 1.0 N A:SER102 4.1 58.1 1.0 CA A:PRO99 4.2 56.9 1.0 C A:LYS103 4.3 55.6 1.0 N A:LYS103 4.4 57.3 1.0 CB A:PRO99 4.6 57.1 1.0 OP2 E:DC11 4.7 40.8 1.0 CA A:LYS103 4.7 56.9 1.0 N A:ALA100 4.8 57.6 1.0 C A:GLY104 5.0 50.1 1.0

M.H.Lamers, A.Perrakis, J.H.Enzlin, H.H.K.Winterwerp, N.De Wind, T.K.Sixma. The Crystal Structure of Dna Mismatch Repair Protein Muts Binding to A G X T Mismatch Nature V. 407 711 2000.
ISSN: ISSN 0028-0836
PubMed: 11048711
DOI: 10.1038/35037523