Magnesium in PDB 1e6d: Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R)

The structure of Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R), PDB code: 1e6d was solved by J.P.Ridge, P.K.Fyfe, K.E.Mcauley, M.E.Van Brederode, B.Robert, R.Van Grondelle, N.W.Isaacs, R.J.Cogdell, M.R.Jones, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.4 / 2.3
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	141.200, 141.200, 187.300, 90.00, 90.00, 120.00
R / Rfree (%)	17.4 / 20

The structure of Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R) also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R) (pdb code 1e6d). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R), PDB code: 1e6d:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg1301 b:31.4 occ:1.00 MG M:BCL1301 0.0 31.4 1.0 ND M:BCL1301 2.0 29.6 1.0 NB M:BCL1301 2.1 32.7 1.0 NC M:BCL1301 2.1 30.1 1.0 NA M:BCL1301 2.2 31.1 1.0 NE2 M:HIS182 2.4 33.8 1.0 C4D M:BCL1301 3.0 31.5 1.0 C1B M:BCL1301 3.1 32.4 1.0 C1D M:BCL1301 3.1 30.6 1.0 C4C M:BCL1301 3.1 29.6 1.0 C1C M:BCL1301 3.1 30.4 1.0 C4B M:BCL1301 3.1 32.3 1.0 CD2 M:HIS182 3.1 33.5 1.0 C4A M:BCL1301 3.1 31.8 1.0 C1A M:BCL1301 3.2 33.0 1.0 CHB M:BCL1301 3.5 32.0 1.0 CHC M:BCL1301 3.5 31.8 1.0 CHA M:BCL1301 3.5 31.8 1.0 CE1 M:HIS182 3.5 33.5 1.0 CHD M:BCL1301 3.5 28.9 1.0 C3D M:BCL1301 4.2 31.0 1.0 C2D M:BCL1301 4.3 31.4 1.0 C2B M:BCL1301 4.3 33.4 1.0 C3B M:BCL1301 4.3 32.2 1.0 C2C M:BCL1301 4.4 29.9 1.0 C3C M:BCL1301 4.4 29.7 1.0 CG M:HIS182 4.4 34.0 1.0 C3A M:BCL1301 4.5 35.0 1.0 C2A M:BCL1301 4.5 35.2 1.0 ND1 M:HIS182 4.5 35.3 1.0 OBB M:BPH1401 4.9 40.6 1.0 CE2 L:PHE181 4.9 32.8 1.0 CM5 M:SPN1600 4.9 38.5 1.0 CBA M:BCL1301 4.9 43.0 1.0 CBD M:BCL1301 4.9 33.4 1.0

Magnesium binding site 2 out of 4 in the Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1302 b:32.3 occ:1.00 MG L:BCL1302 0.0 32.3 1.0 ND L:BCL1302 2.0 32.5 1.0 NB L:BCL1302 2.1 31.6 1.0 NC L:BCL1302 2.1 32.2 1.0 NA L:BCL1302 2.2 31.0 1.0 NE2 L:HIS173 2.3 24.6 1.0 C4D L:BCL1302 3.0 33.4 1.0 C1C L:BCL1302 3.1 32.0 1.0 C1B L:BCL1302 3.1 29.4 1.0 C4C L:BCL1302 3.1 33.5 1.0 C1D L:BCL1302 3.1 33.6 1.0 C4B L:BCL1302 3.1 31.6 1.0 C4A L:BCL1302 3.2 30.0 1.0 C1A L:BCL1302 3.2 30.7 1.0 CE1 L:HIS173 3.3 25.9 1.0 CD2 L:HIS173 3.4 25.0 1.0 CBB M:BCL1303 3.5 30.0 1.0 CHC L:BCL1302 3.5 31.5 1.0 CHB L:BCL1302 3.5 30.3 1.0 CHD L:BCL1302 3.5 32.8 1.0 CHA L:BCL1302 3.5 31.4 1.0 CAB M:BCL1303 4.1 32.7 1.0 C3D L:BCL1302 4.2 32.1 1.0 C2D L:BCL1302 4.3 33.3 1.0 C2B L:BCL1302 4.3 30.1 1.0 C3B L:BCL1302 4.3 29.8 1.0 C2C L:BCL1302 4.4 32.9 1.0 C3C L:BCL1302 4.4 33.9 1.0 ND1 L:HIS173 4.4 25.2 1.0 C3A L:BCL1302 4.5 31.5 1.0 CG L:HIS173 4.5 26.2 1.0 C2A L:BCL1302 4.5 32.0 1.0 CD2 L:PHE167 4.7 32.9 1.0 C3B M:BCL1303 4.7 30.9 1.0 OBB M:BCL1303 4.8 32.0 1.0 CBD L:BCL1302 5.0 31.1 1.0

Magnesium binding site 3 out of 4 in the Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg1303 b:31.8 occ:1.00 MG M:BCL1303 0.0 31.8 1.0 ND M:BCL1303 2.0 33.0 1.0 NB M:BCL1303 2.1 29.9 1.0 NC M:BCL1303 2.1 32.0 1.0 NA M:BCL1303 2.2 32.1 1.0 NE2 M:HIS202 2.3 29.4 1.0 C4D M:BCL1303 3.0 33.5 1.0 C1B M:BCL1303 3.1 30.7 1.0 C4B M:BCL1303 3.1 31.1 1.0 C1C M:BCL1303 3.1 32.4 1.0 C4C M:BCL1303 3.1 33.0 1.0 C1D M:BCL1303 3.1 33.1 1.0 C4A M:BCL1303 3.2 30.4 1.0 C1A M:BCL1303 3.2 32.5 1.0 CE1 M:HIS202 3.2 29.7 1.0 CD2 M:HIS202 3.3 28.2 1.0 CHC M:BCL1303 3.5 30.3 1.0 CHA M:BCL1303 3.5 33.1 1.0 CHB M:BCL1303 3.5 30.4 1.0 CHD M:BCL1303 3.5 32.8 1.0 CBB L:BCL1302 3.8 30.2 1.0 CAB L:BCL1302 3.8 31.3 1.0 OBB L:BCL1302 4.0 29.4 1.0 C3D M:BCL1303 4.2 33.3 1.0 C2B M:BCL1303 4.3 29.7 1.0 C3B M:BCL1303 4.3 30.9 1.0 C2D M:BCL1303 4.3 33.1 1.0 ND1 M:HIS202 4.4 28.1 1.0 C2C M:BCL1303 4.4 35.4 1.0 C3C M:BCL1303 4.4 35.3 1.0 CG M:HIS202 4.4 27.3 1.0 C2A M:BCL1303 4.5 33.8 1.0 C3A M:BCL1303 4.5 32.8 1.0 C3B L:BCL1302 4.6 29.8 1.0 CBD M:BCL1303 5.0 32.6 1.0

Magnesium binding site 4 out of 4 in the Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Photosynthetic Reaction Center Mutant with Trp M115 Replaced with Phe (Chain M, WM115F) Phe M197 Replaced with Arg (Chain M, FM197R) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg1304 b:28.4 occ:1.00 MG L:BCL1304 0.0 28.4 1.0 ND L:BCL1304 2.0 23.8 1.0 NB L:BCL1304 2.0 24.0 1.0 NC L:BCL1304 2.1 24.0 1.0 NA L:BCL1304 2.2 25.1 1.0 NE2 L:HIS153 2.5 35.3 1.0 C4D L:BCL1304 2.9 23.6 1.0 C1B L:BCL1304 3.1 24.2 1.0 C4B L:BCL1304 3.1 25.6 1.0 C1C L:BCL1304 3.1 26.1 1.0 C1D L:BCL1304 3.1 24.0 1.0 C4C L:BCL1304 3.1 23.6 1.0 C4A L:BCL1304 3.2 25.7 1.0 C1A L:BCL1304 3.2 26.4 1.0 CD2 L:HIS153 3.2 33.9 1.0 CHA L:BCL1304 3.4 25.5 1.0 CHC L:BCL1304 3.4 25.5 1.0 CHB L:BCL1304 3.5 24.0 1.0 CHD L:BCL1304 3.5 25.3 1.0 CE1 L:HIS153 3.6 36.1 1.0 C3D L:BCL1304 4.2 23.2 1.0 C2D L:BCL1304 4.3 24.2 1.0 C2B L:BCL1304 4.3 24.8 1.0 C3B L:BCL1304 4.3 24.9 1.0 C2C L:BCL1304 4.4 26.3 1.0 C3C L:BCL1304 4.4 26.4 1.0 CG L:HIS153 4.4 35.2 1.0 C3A L:BCL1304 4.5 26.3 1.0 C2A L:BCL1304 4.5 28.1 1.0 ND1 L:HIS153 4.6 36.0 1.0 OBB L:BPH1402 4.6 38.4 1.0 CBC L:BCL1304 4.7 23.1 1.0 CE2 M:TYR210 4.8 37.9 1.0 CBA L:BCL1304 4.9 33.4 1.0 CBD L:BCL1304 4.9 29.2 1.0 CD2 L:LEU154 5.0 39.7 1.0

J.P.Ridge, P.K.Fyfe, K.E.Mcauley, M.E.Van Brederode, B.Robert, R.Van Grondelle, N.W.Isaacs, R.J.Cogdell, M.R.Jones. An Examination of How Structural Changes Can Affect the Rate of Electron Transfer in A Mutated Bacterial Photoreaction Centre Biochem.J. V. 351 567 2000.
ISSN: ISSN 0264-6021
PubMed: 11042110
DOI: 10.1042/0264-6021:3510567