Magnesium in PDB 1ew2: Crystal Structure of A Human Phosphatase

All present enzymatic activity of Crystal Structure of A Human Phosphatase:
3.1.3.1;

The structure of Crystal Structure of A Human Phosphatase, PDB code: 1ew2 was solved by M.H.Le Du, T.Stigbrand, M.J.Taussig, A.Menez, E.A.Stura, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	22.00 / 1.82
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	88.800, 114.500, 106.900, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 24.2

The structure of Crystal Structure of A Human Phosphatase also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of A Human Phosphatase (pdb code 1ew2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of A Human Phosphatase, PDB code: 1ew2:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of A Human Phosphatase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of A Human Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1003 b:14.5 occ:1.00 OE2 A:GLU311 2.3 15.4 1.0 OD2 A:ASP42 2.3 13.3 1.0 O A:HOH1115 2.4 14.3 1.0 O A:HOH1006 2.4 15.3 1.0 OG A:SER155 2.4 13.9 1.0 O A:HOH1426 2.5 16.3 1.0 CG A:ASP42 3.1 13.7 1.0 CD A:GLU311 3.2 15.8 1.0 CB A:SER155 3.3 11.9 1.0 OE1 A:GLU311 3.5 14.8 1.0 CB A:ASP42 3.6 12.8 1.0 OG A:SER92 3.9 23.3 1.0 N A:SER155 4.1 13.1 1.0 O A:HOH1076 4.1 15.5 1.0 OD1 A:ASP42 4.2 13.3 1.0 CD2 A:HIS153 4.2 15.0 1.0 CA A:SER155 4.4 12.0 1.0 CG A:GLU311 4.5 14.9 1.0 O3 A:PO41005 4.5 21.6 1.0 O A:GLY313 4.6 13.4 1.0 CB A:SER92 4.7 19.1 1.0 CA A:GLY313 4.7 11.4 1.0 O A:HOH1097 4.7 13.8 1.0 CG2 A:THR149 4.7 19.0 1.0 ZN A:ZN1002 4.8 19.2 1.0 OG1 A:THR149 4.8 17.1 1.0 CD A:PRO156 4.9 11.7 1.0 NE2 A:HIS153 4.9 17.3 1.0 CA A:ASP42 5.0 10.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of A Human Phosphatase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of A Human Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1004 b:15.7 occ:1.00 OE2 A:GLU270 2.4 17.6 1.0 O A:PHE269 2.4 14.5 1.0 OE1 A:GLU216 2.4 18.5 1.0 O A:HOH1428 2.4 20.2 1.0 OD2 A:ASP285 2.5 21.9 1.0 OE2 A:GLU216 2.6 17.5 1.0 OD1 A:ASP285 2.6 18.9 1.0 CD A:GLU216 2.9 20.3 1.0 CG A:ASP285 2.9 19.1 1.0 CD A:GLU270 3.3 16.7 1.0 C A:PHE269 3.6 14.3 1.0 CG A:GLU270 3.8 14.9 1.0 O A:HOH1107 3.8 29.9 1.0 OE1 A:GLU270 4.2 16.4 1.0 CA A:PHE269 4.3 14.7 1.0 CB A:PHE269 4.4 15.4 1.0 CG A:GLU216 4.4 18.4 1.0 O A:LEU284 4.4 18.4 1.0 CB A:ASP285 4.4 17.1 1.0 N A:GLU270 4.4 14.5 1.0 O A:TRP248 4.5 18.1 1.0 CA A:GLU270 4.5 13.4 1.0 O A:HOH1045 4.5 17.8 1.0 CB A:LEU284 4.7 20.7 1.0 NH2 A:ARG204 4.7 17.2 1.0 C A:LEU284 4.7 17.8 1.0 OH A:TYR217 4.7 22.1 1.0 CB A:GLU270 4.8 15.4 1.0 NH1 A:ARG204 4.8 15.7 1.0 O A:HOH1192 4.8 19.3 1.0 CA A:ASP285 4.8 15.9 1.0 O A:HOH1560 4.8 48.9 1.0 CB A:GLU216 4.9 16.8 1.0 N A:ASP285 4.9 16.5 1.0

M.H.Le Du, T.Stigbrand, M.J.Taussig, A.Menez, E.A.Stura. Crystal Structure of Alkaline Phosphatase From Human Placenta at 1.8 A Resolution. Implication For A Substrate Specificity. J.Biol.Chem. V. 276 9158 2001.
ISSN: ISSN 0021-9258
PubMed: 11124260
DOI: 10.1074/JBC.M009250200