Magnesium in PDB 1f6n: Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides

The structure of Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides, PDB code: 1f6n was solved by A.Kuglstatter, U.Ermler, H.Michel, L.Baciou, G.Fritzsch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.80
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	141.535, 141.535, 187.913, 90.00, 90.00, 120.00
R / Rfree (%)	22.1 / 25

The structure of Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides (pdb code 1f6n). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides, PDB code: 1f6n:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg301 b:60.8 occ:1.00 MG L:BCL301 0.0 60.8 1.0 ND L:BCL301 2.0 60.7 1.0 NB L:BCL301 2.1 61.2 1.0 NC L:BCL301 2.1 60.6 1.0 NA L:BCL301 2.2 61.2 1.0 NE2 M:HIS182 2.2 61.0 1.0 C4D L:BCL301 3.0 60.7 1.0 C4B L:BCL301 3.1 61.1 1.0 C1D L:BCL301 3.1 60.5 1.0 C1B L:BCL301 3.1 61.1 1.0 C1C L:BCL301 3.1 60.6 1.0 C4C L:BCL301 3.1 60.5 1.0 CE1 M:HIS182 3.1 61.2 1.0 C4A L:BCL301 3.2 61.3 1.0 C1A L:BCL301 3.2 61.5 1.0 CD2 M:HIS182 3.2 61.4 1.0 CHC L:BCL301 3.4 60.7 1.0 CHD L:BCL301 3.5 60.5 1.0 CHA L:BCL301 3.5 61.0 1.0 CHB L:BCL301 3.5 60.9 1.0 C3D L:BCL301 4.2 60.5 1.0 C2D L:BCL301 4.3 60.6 1.0 C2B L:BCL301 4.3 61.3 1.0 C3B L:BCL301 4.3 61.3 1.0 ND1 M:HIS182 4.3 61.6 1.0 C2C L:BCL301 4.4 60.2 1.0 CG M:HIS182 4.4 61.7 1.0 C3C L:BCL301 4.4 60.2 1.0 C3A L:BCL301 4.5 61.7 1.0 C2A L:BCL301 4.5 62.3 1.0 CE2 L:PHE181 4.8 56.7 1.0 C18 M:SPO600 4.9 74.1 1.0 C19 M:SPO600 5.0 73.5 1.0 CBD L:BCL301 5.0 60.7 1.0 OBB M:BPH401 5.0 77.3 1.0 CAC L:BCL301 5.0 60.2 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg302 b:52.6 occ:1.00 MG L:BCL302 0.0 52.6 1.0 ND L:BCL302 2.0 53.1 1.0 NB L:BCL302 2.1 51.4 1.0 NC L:BCL302 2.1 52.2 1.0 NA L:BCL302 2.2 52.6 1.0 NE2 L:HIS173 2.5 54.2 1.0 C4D L:BCL302 3.0 53.2 1.0 C1B L:BCL302 3.1 51.1 1.0 C4B L:BCL302 3.1 51.2 1.0 C1D L:BCL302 3.1 53.1 1.0 C1C L:BCL302 3.1 52.2 1.0 C4C L:BCL302 3.1 52.3 1.0 C4A L:BCL302 3.2 52.2 1.0 C1A L:BCL302 3.2 52.6 1.0 CE1 L:HIS173 3.3 53.9 1.0 CHC L:BCL302 3.4 52.0 1.0 CHB L:BCL302 3.5 51.4 1.0 CD2 L:HIS173 3.5 54.3 1.0 CHD L:BCL302 3.5 52.6 1.0 CHA L:BCL302 3.5 53.0 1.0 CBB M:BCL801 3.6 55.5 1.0 OBB M:BCL801 3.7 55.8 1.0 CAB M:BCL801 3.7 55.1 1.0 C3D L:BCL302 4.2 53.1 1.0 C2D L:BCL302 4.3 53.4 1.0 C2B L:BCL302 4.3 50.8 1.0 C3B L:BCL302 4.3 50.6 1.0 C2C L:BCL302 4.4 52.0 1.0 C3C L:BCL302 4.4 52.3 1.0 C3A L:BCL302 4.5 52.5 1.0 C3B M:BCL801 4.5 54.3 1.0 C2A L:BCL302 4.5 52.3 1.0 ND1 L:HIS173 4.6 54.4 1.0 CG L:HIS173 4.6 54.7 1.0 CD1 L:PHE167 4.8 53.9 1.0 CBD L:BCL302 5.0 53.4 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg304 b:40.5 occ:1.00 MG L:BCL304 0.0 40.5 1.0 ND L:BCL304 2.0 39.5 1.0 NC L:BCL304 2.0 39.1 1.0 NB L:BCL304 2.1 40.0 1.0 NA L:BCL304 2.3 40.8 1.0 NE2 L:HIS153 2.3 45.0 1.0 CHB L:BCL304 2.7 40.1 1.0 C4D L:BCL304 3.0 39.5 1.0 C1B L:BCL304 3.0 39.8 1.0 C1D L:BCL304 3.0 39.0 1.0 C4C L:BCL304 3.0 38.9 1.0 C1C L:BCL304 3.1 39.6 1.0 C4A L:BCL304 3.1 40.7 1.0 C4B L:BCL304 3.2 39.7 1.0 CE1 L:HIS153 3.2 47.1 1.0 C1A L:BCL304 3.3 41.2 1.0 CD2 L:HIS153 3.3 48.1 1.0 CHD L:BCL304 3.4 38.7 1.0 CHC L:BCL304 3.6 39.6 1.0 CHA L:BCL304 3.6 40.7 1.0 C3D L:BCL304 4.2 39.2 1.0 C2D L:BCL304 4.2 38.9 1.0 C2B L:BCL304 4.3 39.4 1.0 C3C L:BCL304 4.3 39.7 1.0 C2C L:BCL304 4.3 39.8 1.0 C3B L:BCL304 4.4 40.0 1.0 ND1 L:HIS153 4.4 50.0 1.0 CBC L:BCL304 4.5 38.9 1.0 C3A L:BCL304 4.5 41.4 1.0 CG L:HIS153 4.5 51.0 1.0 C2A L:BCL304 4.6 42.1 1.0 CE2 M:TYR210 4.6 51.8 1.0 OBB L:BPH402 4.7 52.3 1.0 CD2 L:LEU154 4.7 60.0 1.0 CMC L:BCL304 4.9 40.5 1.0 CAC L:BCL304 4.9 39.6 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure Analysis of the Mutant Reaction Center Pro L209-> Tyr From the Photosynthetic Purple Bacterium Rhodobacter Sphaeroides within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg801 b:53.0 occ:1.00 MG M:BCL801 0.0 53.0 1.0 ND M:BCL801 2.0 53.3 1.0 NB M:BCL801 2.1 53.3 1.0 NC M:BCL801 2.1 53.2 1.0 NA M:BCL801 2.2 53.5 1.0 NE2 M:HIS202 2.3 53.3 1.0 C4D M:BCL801 2.9 53.5 1.0 C1B M:BCL801 3.1 53.3 1.0 C4B M:BCL801 3.1 53.5 1.0 C1C M:BCL801 3.1 53.4 1.0 C1D M:BCL801 3.1 53.2 1.0 C4C M:BCL801 3.1 53.1 1.0 C4A M:BCL801 3.2 53.6 1.0 C1A M:BCL801 3.2 53.6 1.0 CE1 M:HIS202 3.2 53.0 1.0 CD2 M:HIS202 3.3 53.1 1.0 CHC M:BCL801 3.4 53.3 1.0 CHA M:BCL801 3.4 53.6 1.0 CHB M:BCL801 3.5 53.3 1.0 CHD M:BCL801 3.5 53.2 1.0 OBB L:BCL302 3.6 50.6 1.0 CAB L:BCL302 3.7 49.8 1.0 CBB L:BCL302 4.1 49.4 1.0 C3D M:BCL801 4.2 53.5 1.0 C2B M:BCL801 4.3 53.8 1.0 C3B M:BCL801 4.3 54.3 1.0 C2D M:BCL801 4.3 53.2 1.0 C2C M:BCL801 4.3 53.4 1.0 C3C M:BCL801 4.4 53.2 1.0 ND1 M:HIS202 4.4 53.1 1.0 C3A M:BCL801 4.5 54.0 1.0 C2A M:BCL801 4.5 54.0 1.0 CG M:HIS202 4.5 53.3 1.0 C3B L:BCL302 4.5 50.6 1.0 CBD M:BCL801 4.9 54.0 1.0 CMC M:BCL801 4.9 53.3 1.0

A.Kuglstatter, U.Ermler, H.Michel, L.Baciou, G.Fritzsch. X-Ray Structure Analyses of Photosynthetic Reaction Center Variants From Rhodobacter Sphaeroides: Structural Changes Induced By Point Mutations at Position L209 Modulate Electron and Proton Transfer. Biochemistry V. 40 4253 2001.
ISSN: ISSN 0006-2960
PubMed: 11284681
DOI: 10.1021/BI001589H