Atomistry » Magnesium » PDB 1fp6-1g7t » 1fqj
Atomistry »
  Magnesium »
    PDB 1fp6-1g7t »
      1fqj »

Magnesium in PDB 1fqj: Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

Enzymatic activity of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

All present enzymatic activity of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]:
3.1.4.35;

Protein crystallography data

The structure of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)], PDB code: 1fqj was solved by K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.02
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 91.609, 115.937, 134.188, 90.00, 90.00, 90.00
R / Rfree (%) 23.3 / 26.5

Other elements in 1fqj:

The structure of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] also contains other interesting chemical elements:

Fluorine (F) 8 atoms
Aluminium (Al) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] (pdb code 1fqj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)], PDB code: 1fqj:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 1fqj

Go back to Magnesium Binding Sites List in 1fqj
Magnesium binding site 1 out of 2 in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg352

b:17.1
occ:1.00
 F4 A:ALF362 2.2 21.6 1.0
O A:HOH364 2.2 10.1 1.0
OG1 A:THR177 2.2 20.0 1.0
O2B A:GDP360 2.2 10.4 1.0
OG A:SER43 2.2 17.7 1.0
O A:HOH365 2.3 14.3 1.0
CB A:SER43 3.1 11.4 1.0
CB A:THR177 3.1 21.9 1.0
F1 A:ALF362 3.1 25.1 1.0
AL A:ALF362 3.4 17.7 1.0
PB A:GDP360 3.5 15.0 1.0
N A:SER43 3.8 13.4 1.0
O3B A:GDP360 3.9 15.2 1.0
OD2 A:ASP196 4.0 30.3 1.0
O A:HOH497 4.0 39.5 1.0
CA A:SER43 4.0 16.9 1.0
CG2 A:THR177 4.1 26.5 1.0
N A:THR177 4.1 12.1 1.0
O2A A:GDP360 4.2 12.7 1.0
CA A:THR177 4.2 14.9 1.0
OD1 A:ASP196 4.4 33.7 1.0
F2 A:ALF362 4.4 16.4 1.0
O A:HOH363 4.4 12.2 1.0
O3A A:GDP360 4.5 11.6 1.0
O1B A:GDP360 4.5 16.2 1.0
O A:VAL175 4.5 17.2 1.0
O A:VAL197 4.5 16.9 1.0
CG A:ASP196 4.6 37.4 1.0
PA A:GDP360 4.7 11.7 1.0
O1A A:GDP360 4.8 11.7 1.0
CB A:LYS42 4.8 9.9 1.0
NH1 A:ARG174 4.9 7.9 1.0
C A:LYS42 4.9 12.3 1.0
O A:HOH493 4.9 26.1 1.0

Magnesium binding site 2 out of 2 in 1fqj

Go back to Magnesium Binding Sites List in 1fqj
Magnesium binding site 2 out of 2 in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg352

b:22.7
occ:1.00
 O D:HOH365 2.1 8.4 1.0
OG D:SER43 2.1 15.1 1.0
F1 D:ALF363 2.2 14.0 1.0
O D:HOH366 2.3 15.5 1.0
O2B D:GDP361 2.3 15.1 1.0
OG1 D:THR177 2.3 18.4 1.0
CB D:SER43 3.0 20.8 1.0
F4 D:ALF363 3.1 18.8 1.0
CB D:THR177 3.2 14.4 1.0
AL D:ALF363 3.5 16.5 1.0
PB D:GDP361 3.6 20.4 1.0
N D:SER43 3.8 19.8 1.0
O2A D:GDP361 3.9 14.5 1.0
CA D:SER43 4.0 16.2 1.0
N D:THR177 4.0 10.0 1.0
O D:HOH383 4.1 23.9 1.0
OD2 D:ASP196 4.2 33.9 1.0
CA D:THR177 4.2 15.2 1.0
CG2 D:THR177 4.3 12.4 1.0
O3B D:GDP361 4.3 29.6 1.0
O3A D:GDP361 4.4 15.2 1.0
OD1 D:ASP196 4.4 31.5 1.0
O D:VAL175 4.4 15.8 1.0
PA D:GDP361 4.5 20.1 1.0
O D:VAL197 4.6 28.8 1.0
O1B D:GDP361 4.6 15.2 1.0
O1A D:GDP361 4.6 18.2 1.0
F3 D:ALF363 4.6 20.8 1.0
O D:HOH392 4.7 32.3 1.0
NH1 D:ARG174 4.7 11.4 1.0
O D:HOH364 4.7 12.2 1.0
CG D:ASP196 4.7 44.0 1.0
CB D:LYS42 4.9 21.3 1.0
C D:LYS42 4.9 22.6 1.0
CE D:LYS42 5.0 17.1 1.0

Reference:

K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler. Structural Determinants For Regulation of Phosphodiesterase By A G Protein at 2.0 A. Nature V. 409 1071 2001.
ISSN: ISSN 0028-0836
PubMed: 11234020
DOI: 10.1038/35059138
Page generated: Tue Aug 13 03:34:49 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy