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Magnesium in PDB 1fqj: Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

Enzymatic activity of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]

All present enzymatic activity of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]:
3.1.4.35;

Protein crystallography data

The structure of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)], PDB code: 1fqj was solved by K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.02
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 91.609, 115.937, 134.188, 90.00, 90.00, 90.00
R / Rfree (%) 23.3 / 26.5

Other elements in 1fqj:

The structure of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] also contains other interesting chemical elements:

Fluorine (F) 8 atoms
Aluminium (Al) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] (pdb code 1fqj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)], PDB code: 1fqj:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 1fqj

Go back to Magnesium Binding Sites List in 1fqj
Magnesium binding site 1 out of 2 in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg352

b:17.1
occ:1.00
 F4 A:ALF362 2.2 21.6 1.0
O A:HOH364 2.2 10.1 1.0
OG1 A:THR177 2.2 20.0 1.0
O2B A:GDP360 2.2 10.4 1.0
OG A:SER43 2.2 17.7 1.0
O A:HOH365 2.3 14.3 1.0
CB A:SER43 3.1 11.4 1.0
CB A:THR177 3.1 21.9 1.0
F1 A:ALF362 3.1 25.1 1.0
AL A:ALF362 3.4 17.7 1.0
PB A:GDP360 3.5 15.0 1.0
N A:SER43 3.8 13.4 1.0
O3B A:GDP360 3.9 15.2 1.0
OD2 A:ASP196 4.0 30.3 1.0
O A:HOH497 4.0 39.5 1.0
CA A:SER43 4.0 16.9 1.0
CG2 A:THR177 4.1 26.5 1.0
N A:THR177 4.1 12.1 1.0
O2A A:GDP360 4.2 12.7 1.0
CA A:THR177 4.2 14.9 1.0
OD1 A:ASP196 4.4 33.7 1.0
F2 A:ALF362 4.4 16.4 1.0
O A:HOH363 4.4 12.2 1.0
O3A A:GDP360 4.5 11.6 1.0
O1B A:GDP360 4.5 16.2 1.0
O A:VAL175 4.5 17.2 1.0
O A:VAL197 4.5 16.9 1.0
CG A:ASP196 4.6 37.4 1.0
PA A:GDP360 4.7 11.7 1.0
O1A A:GDP360 4.8 11.7 1.0
CB A:LYS42 4.8 9.9 1.0
NH1 A:ARG174 4.9 7.9 1.0
C A:LYS42 4.9 12.3 1.0
O A:HOH493 4.9 26.1 1.0

Magnesium binding site 2 out of 2 in 1fqj

Go back to Magnesium Binding Sites List in 1fqj
Magnesium binding site 2 out of 2 in the Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)]


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Heterotrimeric Complex of the Rgs Domain of RGS9, the Gamma Subunit of Phosphodiesterase and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Pdegamma)-(Gt/I1ALPHA)-(Gdp)-(ALF4-)-(MG2+)] within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg352

b:22.7
occ:1.00
 O D:HOH365 2.1 8.4 1.0
OG D:SER43 2.1 15.1 1.0
F1 D:ALF363 2.2 14.0 1.0
O D:HOH366 2.3 15.5 1.0
O2B D:GDP361 2.3 15.1 1.0
OG1 D:THR177 2.3 18.4 1.0
CB D:SER43 3.0 20.8 1.0
F4 D:ALF363 3.1 18.8 1.0
CB D:THR177 3.2 14.4 1.0
AL D:ALF363 3.5 16.5 1.0
PB D:GDP361 3.6 20.4 1.0
N D:SER43 3.8 19.8 1.0
O2A D:GDP361 3.9 14.5 1.0
CA D:SER43 4.0 16.2 1.0
N D:THR177 4.0 10.0 1.0
O D:HOH383 4.1 23.9 1.0
OD2 D:ASP196 4.2 33.9 1.0
CA D:THR177 4.2 15.2 1.0
CG2 D:THR177 4.3 12.4 1.0
O3B D:GDP361 4.3 29.6 1.0
O3A D:GDP361 4.4 15.2 1.0
OD1 D:ASP196 4.4 31.5 1.0
O D:VAL175 4.4 15.8 1.0
PA D:GDP361 4.5 20.1 1.0
O D:VAL197 4.6 28.8 1.0
O1B D:GDP361 4.6 15.2 1.0
O1A D:GDP361 4.6 18.2 1.0
F3 D:ALF363 4.6 20.8 1.0
O D:HOH392 4.7 32.3 1.0
NH1 D:ARG174 4.7 11.4 1.0
O D:HOH364 4.7 12.2 1.0
CG D:ASP196 4.7 44.0 1.0
CB D:LYS42 4.9 21.3 1.0
C D:LYS42 4.9 22.6 1.0
CE D:LYS42 5.0 17.1 1.0

Reference:

K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler. Structural Determinants For Regulation of Phosphodiesterase By A G Protein at 2.0 A. Nature V. 409 1071 2001.
ISSN: ISSN 0028-0836
PubMed: 11234020
DOI: 10.1038/35059138
Page generated: Tue Aug 13 03:34:49 2024

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