Magnesium in PDB 1fqk: Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)]

The structure of Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)], PDB code: 1fqk was solved by K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	96.785, 115.073, 136.460, 90.00, 90.00, 90.00
R / Rfree (%)	23.1 / 26.8

The structure of Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)] also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Aluminium	(Al)	2 atoms

The binding sites of Magnesium atom in the Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)] (pdb code 1fqk). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)], PDB code: 1fqk:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)]


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)] within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg352 b:47.9 occ:1.00 O A:HOH364 2.2 44.7 1.0 OG A:SER43 2.3 53.8 1.0 F1 A:ALF362 2.4 55.0 1.0 OG1 A:THR177 2.4 58.8 1.0 O A:HOH365 2.4 49.8 1.0 O2B A:GDP360 2.6 53.9 1.0 CG2 A:THR177 2.8 61.7 1.0 CB A:SER43 2.9 49.8 1.0 CB A:THR177 3.1 57.3 1.0 F4 A:ALF362 3.1 50.2 1.0 AL A:ALF362 3.7 51.9 1.0 N A:THR177 3.9 49.1 1.0 PB A:GDP360 3.9 56.3 1.0 N A:SER43 3.9 52.1 1.0 O2A A:GDP360 4.0 52.0 1.0 CA A:SER43 4.0 51.2 1.0 CA A:THR177 4.1 51.2 1.0 O A:VAL175 4.1 45.7 1.0 OD1 A:ASP196 4.2 62.3 1.0 O3B A:GDP360 4.6 57.0 1.0 O A:HOH408 4.6 57.4 1.0 OD2 A:ASP196 4.7 57.3 1.0 O3A A:GDP360 4.7 54.3 1.0 PA A:GDP360 4.7 50.7 1.0 NH1 A:ARG174 4.7 45.0 1.0 O A:VAL197 4.7 58.7 1.0 O1A A:GDP360 4.8 48.7 1.0 O A:HOH363 4.8 56.8 1.0 F3 A:ALF362 4.8 49.8 1.0 CG A:ASP196 4.9 61.7 1.0 O1B A:GDP360 4.9 60.8 1.0 C A:LYS176 5.0 47.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)]


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Heterodimeric Complex of the Rgs Domain of RGS9, and the Gt/I1 Chimera Alpha Subunit [(RGS9)-(Gt/I1ALPHA)-(Gdp)- (ALF4-)-(MG2+)] within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg352 b:58.0 occ:1.00 O C:HOH366 2.3 45.5 1.0 OG C:SER43 2.3 60.2 1.0 F1 C:ALF363 2.3 52.2 1.0 O2B C:GDP361 2.3 55.7 1.0 OG1 C:THR177 2.4 49.8 1.0 O C:HOH365 2.4 40.8 1.0 CB C:SER43 2.7 53.3 1.0 N C:SER43 3.2 53.2 1.0 CB C:THR177 3.4 46.6 1.0 CA C:SER43 3.4 55.2 1.0 PB C:GDP361 3.7 61.0 1.0 OD1 C:ASP196 3.8 58.7 1.0 AL C:ALF363 3.9 52.5 1.0 OD2 C:ASP196 4.0 57.4 1.0 F4 C:ALF363 4.0 55.4 1.0 O2A C:GDP361 4.2 56.7 1.0 CG2 C:THR177 4.3 42.7 1.0 CG C:ASP196 4.3 60.0 1.0 C C:LYS42 4.3 50.4 1.0 O1B C:GDP361 4.3 67.2 1.0 CB C:LYS42 4.3 40.2 1.0 F3 C:ALF363 4.5 44.4 1.0 O1A C:GDP361 4.6 58.0 1.0 O3A C:GDP361 4.6 58.3 1.0 N C:THR177 4.6 44.6 1.0 O C:VAL197 4.6 52.8 1.0 O3B C:GDP361 4.6 66.0 1.0 CA C:THR177 4.6 45.2 1.0 PA C:GDP361 4.7 50.2 1.0 C C:SER43 4.8 57.1 1.0 CA C:LYS42 4.8 46.7 1.0 O C:VAL175 4.9 49.0 1.0 CE C:LYS42 4.9 41.6 1.0 O C:HOH364 5.0 49.8 1.0

K.C.Slep, M.A.Kercher, W.He, C.W.Cowan, T.G.Wensel, P.B.Sigler. Structural Determinants For Regulation of Phosphodiesterase By A G Protein at 2.0 A. Nature V. 409 1071 2001.
ISSN: ISSN 0028-0836
PubMed: 11234020
DOI: 10.1038/35059138