Magnesium in PDB 1gk8: Rubisco From Chlamydomonas Reinhardtii

All present enzymatic activity of Rubisco From Chlamydomonas Reinhardtii:
4.1.1.39;

The structure of Rubisco From Chlamydomonas Reinhardtii, PDB code: 1gk8 was solved by T.C.Taylor, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	171.375, 142.638, 124.717, 90.00, 124.13, 90.00
R / Rfree (%)	14.9 / 16.2

The binding sites of Magnesium atom in the Rubisco From Chlamydomonas Reinhardtii (pdb code 1gk8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Rubisco From Chlamydomonas Reinhardtii, PDB code: 1gk8:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Rubisco From Chlamydomonas Reinhardtii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Rubisco From Chlamydomonas Reinhardtii within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1476 b:7.4 occ:1.00 OD1 A:ASP203 2.0 8.6 1.0 OE1 A:GLU204 2.0 7.6 1.0 OQ2 A:KCX201 2.0 7.3 1.0 O6 A:CAP1477 2.1 8.7 1.0 O3 A:CAP1477 2.2 8.0 1.0 O2 A:CAP1477 2.2 8.7 1.0 C A:CAP1477 2.8 8.1 1.0 C2 A:CAP1477 2.8 8.2 1.0 CX A:KCX201 3.0 6.8 1.0 C3 A:CAP1477 3.0 8.6 1.0 CD A:GLU204 3.1 7.4 1.0 CG A:ASP203 3.2 8.7 1.0 OQ1 A:KCX201 3.3 7.4 1.0 OE2 A:GLU204 3.5 8.3 1.0 NZ A:LYS177 3.7 8.4 1.0 N A:GLU204 3.8 6.3 1.0 OD2 A:ASP203 3.9 9.6 1.0 NZ A:LYS175 4.0 8.8 1.0 NE2 A:HIS294 4.0 6.8 1.0 O7 A:CAP1477 4.0 8.5 1.0 CG2 A:THR173 4.0 8.0 1.0 CA A:ASP203 4.1 7.0 1.0 C4 A:CAP1477 4.2 9.1 1.0 CB A:ASP203 4.2 7.0 1.0 NZ A:KCX201 4.2 7.1 1.0 OG1 A:THR173 4.3 7.9 1.0 C1 A:CAP1477 4.3 8.6 1.0 CG A:GLU204 4.3 7.8 1.0 C A:ASP203 4.5 7.3 1.0 CB A:GLU204 4.5 7.3 1.0 CD2 A:HIS294 4.6 6.4 1.0 CB A:THR173 4.6 8.2 1.0 C5 A:CAP1477 4.7 9.3 1.0 CA A:GLU204 4.8 6.8 1.0 O1 A:CAP1477 4.8 8.4 1.0 CE1 A:HIS294 4.9 6.9 1.0

Magnesium binding site 2 out of 4 in the Rubisco From Chlamydomonas Reinhardtii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Rubisco From Chlamydomonas Reinhardtii within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1476 b:6.0 occ:1.00 OD1 C:ASP203 1.9 7.7 1.0 OQ2 C:KCX201 2.0 6.7 1.0 OE1 C:GLU204 2.0 7.0 1.0 O6 C:CAP1477 2.1 6.8 1.0 O3 C:CAP1477 2.2 7.5 1.0 O2 C:CAP1477 2.3 7.8 1.0 C C:CAP1477 2.8 7.7 1.0 C2 C:CAP1477 2.9 7.8 1.0 CX C:KCX201 3.0 6.4 1.0 C3 C:CAP1477 3.1 7.6 1.0 CD C:GLU204 3.1 6.5 1.0 CG C:ASP203 3.1 7.9 1.0 OQ1 C:KCX201 3.3 6.7 1.0 OE2 C:GLU204 3.6 8.1 1.0 NZ C:LYS177 3.7 7.7 1.0 N C:GLU204 3.8 6.4 1.0 OD2 C:ASP203 3.9 8.7 1.0 NZ C:LYS175 4.0 8.0 1.0 NE2 C:HIS294 4.0 6.4 1.0 CG2 C:THR173 4.0 7.5 1.0 O7 C:CAP1477 4.0 7.6 1.0 CA C:ASP203 4.1 6.4 1.0 NZ C:KCX201 4.2 6.6 1.0 CB C:ASP203 4.2 7.2 1.0 C4 C:CAP1477 4.2 7.8 1.0 OG1 C:THR173 4.3 7.8 1.0 C1 C:CAP1477 4.3 8.1 1.0 CG C:GLU204 4.3 7.2 1.0 C C:ASP203 4.5 6.6 1.0 CB C:GLU204 4.5 6.8 1.0 CD2 C:HIS294 4.6 6.1 1.0 CB C:THR173 4.6 7.5 1.0 C5 C:CAP1477 4.7 7.9 1.0 CA C:GLU204 4.8 6.5 1.0 O1 C:CAP1477 4.9 8.4 1.0 CE1 C:HIS294 4.9 5.8 1.0

Magnesium binding site 3 out of 4 in the Rubisco From Chlamydomonas Reinhardtii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Rubisco From Chlamydomonas Reinhardtii within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg1476 b:6.3 occ:1.00 OD1 E:ASP203 2.0 7.5 1.0 OQ2 E:KCX201 2.0 6.4 1.0 OE1 E:GLU204 2.0 7.0 1.0 O6 E:CAP1477 2.1 7.2 1.0 O3 E:CAP1477 2.2 7.1 1.0 O2 E:CAP1477 2.2 7.2 1.0 C E:CAP1477 2.8 6.9 1.0 C2 E:CAP1477 2.8 7.5 1.0 CX E:KCX201 3.0 5.5 1.0 C3 E:CAP1477 3.0 7.5 1.0 CD E:GLU204 3.1 6.6 1.0 CG E:ASP203 3.2 7.5 1.0 OQ1 E:KCX201 3.3 7.1 1.0 OE2 E:GLU204 3.6 7.9 1.0 NZ E:LYS177 3.7 7.8 1.0 N E:GLU204 3.8 6.3 1.0 OD2 E:ASP203 3.9 8.4 1.0 NZ E:LYS175 3.9 7.3 1.0 CG2 E:THR173 4.0 6.7 1.0 NE2 E:HIS294 4.0 5.8 1.0 O7 E:CAP1477 4.0 7.4 1.0 CA E:ASP203 4.1 6.5 1.0 NZ E:KCX201 4.2 6.5 1.0 CB E:ASP203 4.2 6.3 1.0 C4 E:CAP1477 4.2 8.3 1.0 OG1 E:THR173 4.3 7.6 1.0 C1 E:CAP1477 4.3 7.4 1.0 CG E:GLU204 4.3 7.1 1.0 CB E:GLU204 4.5 6.9 1.0 C E:ASP203 4.5 6.8 1.0 CD2 E:HIS294 4.6 6.3 1.0 CB E:THR173 4.6 7.0 1.0 C5 E:CAP1477 4.7 8.1 1.0 O1 E:CAP1477 4.8 7.2 1.0 CA E:GLU204 4.8 6.3 1.0 CE1 E:HIS294 4.9 6.3 1.0

Magnesium binding site 4 out of 4 in the Rubisco From Chlamydomonas Reinhardtii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Rubisco From Chlamydomonas Reinhardtii within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg1476 b:6.8 occ:1.00 OD1 G:ASP203 1.9 8.4 1.0 OE1 G:GLU204 2.0 7.5 1.0 OQ2 G:KCX201 2.0 7.5 1.0 O6 G:CAP1477 2.1 7.4 1.0 O3 G:CAP1477 2.2 7.8 1.0 O2 G:CAP1477 2.2 8.2 1.0 C2 G:CAP1477 2.8 8.2 1.0 C G:CAP1477 2.8 7.9 1.0 C3 G:CAP1477 3.0 8.3 1.0 CX G:KCX201 3.0 6.6 1.0 CD G:GLU204 3.1 7.5 1.0 CG G:ASP203 3.1 8.6 1.0 OQ1 G:KCX201 3.3 8.2 1.0 OE2 G:GLU204 3.6 8.8 1.0 NZ G:LYS177 3.7 8.2 1.0 N G:GLU204 3.8 7.0 1.0 OD2 G:ASP203 3.9 9.7 1.0 NZ G:LYS175 3.9 8.4 1.0 NE2 G:HIS294 4.0 7.1 1.0 O7 G:CAP1477 4.0 9.0 1.0 CG2 G:THR173 4.0 8.3 1.0 CA G:ASP203 4.1 7.2 1.0 CB G:ASP203 4.2 8.1 1.0 C4 G:CAP1477 4.2 8.9 1.0 NZ G:KCX201 4.2 6.7 1.0 C1 G:CAP1477 4.3 8.6 1.0 OG1 G:THR173 4.3 8.6 1.0 CG G:GLU204 4.3 7.3 1.0 C G:ASP203 4.5 7.5 1.0 CB G:GLU204 4.5 7.4 1.0 CD2 G:HIS294 4.6 6.7 1.0 CB G:THR173 4.6 8.3 1.0 C5 G:CAP1477 4.7 8.9 1.0 CA G:GLU204 4.8 7.0 1.0 O1 G:CAP1477 4.8 7.8 1.0 CE1 G:HIS294 4.9 6.5 1.0

T.C.Taylor, A.Backlund, K.Bjorhall, R.J.Spreitzer, I.Andersson. First Crystal Structure of Rubisco From A Green Alga, Chlamydomonas Reinhardtii. J.Biol.Chem. V. 276 48159 2001.
ISSN: ISSN 0021-9258
PubMed: 11641402
DOI: 10.1074/JBC.M107765200