Magnesium in PDB 1h56: Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii

All present enzymatic activity of Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii:
3.1.21.4;

The structure of Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii, PDB code: 1h56 was solved by A.Spyrida, C.Matzen, T.Lanio, A.Jeltsch, A.Simoncsits, A.Athanasiadis, E.Scheuring-Vanamee, M.Kokkinidis, A.Pingoud, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 3.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	84.180, 105.280, 46.670, 90.00, 90.00, 90.00
R / Rfree (%)	15 / n/a

The binding sites of Magnesium atom in the Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii (pdb code 1h56). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii, PDB code: 1h56:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1 b:81.0 occ:1.00 O A:HOH2014 1.9 73.8 1.0 OE1 A:GLU68 2.4 63.1 1.0 O A:HOH2016 2.7 38.4 1.0 OE2 A:GLU68 2.7 56.6 1.0 CD A:GLU68 2.9 52.2 1.0 OD2 A:ASP58 3.0 53.5 1.0 O A:HOH2025 3.6 52.4 1.0 CE A:LYS70 3.7 39.2 1.0 CG A:ASP58 3.9 42.6 1.0 O A:GLY56 3.9 0.5 1.0 OD1 A:ASP58 4.0 54.9 1.0 OH A:TYR94 4.2 65.0 1.0 CG A:GLU68 4.3 35.3 1.0 NZ A:LYS70 4.3 40.9 1.0 CE1 A:TYR94 4.5 56.9 1.0 CD A:LYS70 4.5 35.1 1.0 N A:LEU69 4.6 17.2 1.0 CZ A:TYR94 4.7 60.5 1.0 O A:LEU69 4.7 20.6 1.0 CB A:GLU68 4.8 23.0 1.0 OE1 A:GLU55 4.9 0.7 1.0 CA A:GLU68 4.9 23.6 1.0

Magnesium binding site 2 out of 2 in the Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structural and Biochemical Characterization of A New Magnesium Ion Binding Site Near TYR94 in the Restriction Endonuclease Pvuii within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1 b:40.3 occ:1.00 OH B:TYR94 2.3 50.5 1.0 O B:THR82 2.7 59.3 1.0 O B:HOH2013 2.8 64.0 1.0 CB B:HIS83 3.2 61.4 1.0 O B:HOH2005 3.2 60.6 1.0 CG B:HIS83 3.5 66.3 1.0 ND1 B:HIS83 3.5 72.4 1.0 C B:THR82 3.5 51.2 1.0 CZ B:TYR94 3.6 49.9 1.0 NZ B:LYS70 3.7 33.6 1.0 CA B:HIS83 3.7 41.0 1.0 N B:HIS83 4.0 39.2 1.0 CE1 B:TYR94 4.1 45.5 1.0 CE1 B:HIS83 4.4 65.0 1.0 CD2 B:HIS83 4.4 70.2 1.0 CA B:THR82 4.7 45.5 1.0 CE2 B:TYR94 4.7 47.6 1.0 O B:HOH2017 4.7 46.4 1.0 NE2 B:HIS83 4.9 68.8 1.0 CE B:LYS70 4.9 33.4 1.0 OE1 B:GLU68 4.9 60.8 1.0 CG2 B:ILE90 5.0 22.4 1.0

A.Spyridaki, C.Matzen, T.Lanio, A.Jeltsch, A.Simoncsits, A.Athanasiadis, E.Scheuring-Vanamee, M.Kokkinidis, A.Pingoud. Structural and Biochemical Characterization of A New Mg(2+) Binding Site Near TYR94 in the Restriction Endonuclease Pvuii. J.Mol.Biol. V. 331 395 2003.
ISSN: ISSN 0022-2836
PubMed: 12888347
DOI: 10.1016/S0022-2836(03)00692-2