Magnesium in PDB 1hi8: Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6

The structure of Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6, PDB code: 1hi8 was solved by J.M.Grimes, S.J.Butcher, E.V.Makeyev, D.H.Bamford, D.I.Stuart, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.50
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	110.000, 110.000, 159.300, 90.00, 90.00, 120.00
R / Rfree (%)	28 / 31.6

The binding sites of Magnesium atom in the Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6 (pdb code 1hi8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6, PDB code: 1hi8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg665 b:42.4 occ:1.00 OD1 A:ASP454 2.3 53.5 1.0 O A:ALA495 2.3 40.3 1.0 OE1 A:GLU491 2.5 27.2 1.0 OE2 A:GLU491 2.9 37.1 1.0 CD A:GLU491 3.0 48.1 1.0 CG A:ASP454 3.0 14.9 1.0 OD2 A:ASP454 3.3 47.6 1.0 C A:ALA495 3.5 25.5 1.0 O A:THR323 4.0 46.1 1.0 CB A:ASP454 4.2 52.5 1.0 CA A:PHE496 4.3 39.5 1.0 N A:PHE496 4.3 20.7 1.0 N A:ALA495 4.3 36.5 1.0 CG A:GLU491 4.3 29.6 1.0 CA A:ALA495 4.4 32.8 1.0 CA A:ASP454 4.5 29.3 1.0 CB A:GLU491 4.7 47.3 1.0 N A:ALA455 4.7 28.3 1.0 CB A:ALA495 4.7 18.5 1.0 O A:ALA455 4.8 36.4 1.0

Magnesium binding site 2 out of 2 in the Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Rna Dependent Rna Polymerase From Dsrna Bacteriophage PHI6 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg665 b:42.4 occ:1.00 OD1 B:ASP454 2.3 53.5 1.0 O B:ALA495 2.3 40.3 1.0 OE1 B:GLU491 2.5 27.2 1.0 OE2 B:GLU491 2.9 37.1 1.0 CD B:GLU491 3.0 48.1 1.0 CG B:ASP454 3.0 14.9 1.0 OD2 B:ASP454 3.3 47.6 1.0 C B:ALA495 3.5 25.5 1.0 O B:THR323 4.0 46.1 1.0 CB B:ASP454 4.2 52.5 1.0 CA B:PHE496 4.3 39.5 1.0 N B:PHE496 4.3 20.7 1.0 N B:ALA495 4.3 36.5 1.0 CG B:GLU491 4.3 29.6 1.0 CA B:ALA495 4.4 32.8 1.0 CA B:ASP454 4.5 29.3 1.0 CB B:GLU491 4.7 47.3 1.0 N B:ALA455 4.7 28.3 1.0 CB B:ALA495 4.7 18.5 1.0 O B:ALA455 4.8 36.4 1.0

S.J.Butcher, J.M.Grimes, E.V.Makeyev, D.H.Bamford, D.I.Stuart. A Mechanism For Initiating Rna-Dependent Rna Polymerization Nature V. 410 235 2001.
ISSN: ISSN 0028-0836
PubMed: 11242087
DOI: 10.1038/35065653