Magnesium in PDB 1ir3: Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog

All present enzymatic activity of Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog:
2.7.1.112;

The structure of Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog, PDB code: 1ir3 was solved by S.R.Hubbard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	66.505, 66.505, 139.095, 90.00, 90.00, 120.00
R / Rfree (%)	19.4 / 22.6

The binding sites of Magnesium atom in the Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog (pdb code 1ir3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog, PDB code: 1ir3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:13.7 occ:1.00 O A:HOH32 2.0 12.1 1.0 O2G A:ANP300 2.0 17.9 1.0 O A:HOH52 2.0 11.2 1.0 OD2 A:ASP1150 2.1 12.1 1.0 O2B A:ANP300 2.1 16.2 1.0 OD1 A:ASN1137 2.2 13.2 1.0 CG A:ASP1150 3.1 13.2 1.0 PG A:ANP300 3.1 17.5 1.0 PB A:ANP300 3.2 15.8 1.0 CG A:ASN1137 3.2 10.5 1.0 N3B A:ANP300 3.5 18.4 1.0 O A:HOH74 3.5 18.2 1.0 O3G A:ANP300 3.6 15.0 1.0 MG A:MG302 3.6 12.1 1.0 CB A:ASP1150 3.6 11.1 1.0 ND2 A:ASN1137 3.7 10.4 1.0 O3A A:ANP300 3.7 15.9 1.0 O2A A:ANP300 4.1 16.3 1.0 O A:HOH95 4.1 22.3 1.0 OD1 A:ASP1150 4.2 14.1 1.0 O A:HOH82 4.4 22.2 1.0 O1G A:ANP300 4.4 15.2 1.0 CB A:ASN1137 4.5 10.3 1.0 O1B A:ANP300 4.5 17.4 1.0 PA A:ANP300 4.5 19.0 1.0 O A:ARG1136 4.7 11.8 1.0 CA A:ASN1137 4.8 11.0 1.0 OH B:TYR10 4.8 15.7 1.0 CG A:ARG1136 4.9 8.0 1.0 CD A:ARG1136 4.9 9.7 1.0 O A:HOH75 4.9 22.9 1.0 O A:HOH158 5.0 33.9 1.0 OD2 A:ASP1132 5.0 11.9 1.0

Magnesium binding site 2 out of 2 in the Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Phosphorylated Insulin Receptor Tyrosine Kinase in Complex with Peptide Substrate and Atp Analog within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:12.1 occ:1.00 O A:HOH170 2.3 24.7 1.0 O A:HOH158 2.4 33.9 1.0 O A:HOH95 2.4 22.3 1.0 O A:HOH82 2.4 22.2 1.0 OD2 A:ASP1150 2.4 12.1 1.0 O2B A:ANP300 2.5 16.2 1.0 OD1 A:ASP1150 2.6 14.1 1.0 CG A:ASP1150 2.8 13.2 1.0 MG A:MG301 3.6 13.7 1.0 PB A:ANP300 3.6 15.8 1.0 O1B A:ANP300 3.8 17.4 1.0 O A:HOH32 3.9 12.1 1.0 O A:HOH58 4.0 21.6 1.0 OE2 A:GLU1043 4.1 26.8 1.0 CB A:ASP1150 4.3 11.1 1.0 OE1 A:GLU1047 4.4 25.1 1.0 O2A A:ANP300 4.5 16.3 1.0 O A:HOH52 4.5 11.2 1.0 O3A A:ANP300 4.7 15.9 1.0 O A:HOH75 4.8 22.9 1.0 N3B A:ANP300 4.8 18.4 1.0 OE2 A:GLU1047 4.8 26.9 1.0 NZ A:LYS1030 5.0 25.7 1.0 CD A:GLU1047 5.0 24.5 1.0 CA A:GLY1152 5.0 13.0 1.0

S.R.Hubbard, S.R.Hubbard. N/A N/A.
ISSN: ISSN 0261-4189
PubMed: 9312016
DOI: 10.1093/EMBOJ/16.18.5572