Magnesium in PDB 1jah: H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Magnesium

The structure of H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Magnesium, PDB code: 1jah was solved by T.Schweins, K.Scheffzek, R.Assheuer, A.Wittinghofer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	40.100, 40.100, 160.640, 90.00, 90.00, 120.00
R / Rfree (%)	22 / 29

The binding sites of Magnesium atom in the H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Magnesium (pdb code 1jah). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Magnesium, PDB code: 1jah:

Magnesium binding site 1 out of 1 in the H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Magnesium


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of H-Ras P21 Protein Mutant G12P, Complexed with Guanosine-5'- [Beta,Gamma-Methylene] Triphosphate and Magnesium within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg168 b:16.1 occ:1.00 O2B A:GCP167 2.2 6.9 1.0 O2G A:GCP167 2.2 12.6 1.0 OG1 A:THR35 2.2 12.1 1.0 O A:HOH176 2.3 18.2 1.0 O A:HOH170 2.3 15.6 1.0 OG A:SER17 2.3 7.7 1.0 PB A:GCP167 3.2 8.6 1.0 PG A:GCP167 3.2 11.1 1.0 CB A:SER17 3.2 7.8 1.0 CB A:THR35 3.2 7.5 1.0 C3B A:GCP167 3.3 10.2 1.0 N A:THR35 3.9 9.5 1.0 N A:SER17 3.9 10.2 1.0 O2A A:GCP167 4.1 8.3 1.0 CA A:SER17 4.1 7.6 1.0 O3A A:GCP167 4.1 9.0 1.0 CA A:THR35 4.2 10.7 1.0 O3G A:GCP167 4.2 13.1 1.0 OD2 A:ASP57 4.2 15.5 1.0 O1G A:GCP167 4.2 11.2 1.0 O1B A:GCP167 4.3 7.0 1.0 CG2 A:THR35 4.3 6.8 1.0 OD1 A:ASP57 4.4 11.1 1.0 O A:HOH205 4.4 15.4 1.0 O A:ASP33 4.5 12.4 1.0 PA A:GCP167 4.5 8.8 1.0 O A:THR58 4.5 13.2 1.0 O1A A:GCP167 4.7 5.2 1.0 CE A:LYS16 4.7 7.3 1.0 CB A:LYS16 4.7 6.2 1.0 CG A:ASP57 4.7 12.8 1.0 C A:PRO34 4.8 10.3 1.0 C A:LYS16 5.0 7.9 1.0

T.Schweins, K.Scheffzek, R.Assheuer, A.Wittinghofer. The Role of the Metal Ion in the P21RAS Catalysed Gtp-Hydrolysis: MN2+ Versus MG2+. J.Mol.Biol. V. 266 847 1997.
ISSN: ISSN 0022-2836
PubMed: 9102473
DOI: 10.1006/JMBI.1996.0814