Magnesium in PDB 1jbz: Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH

The structure of Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH, PDB code: 1jbz was solved by G.T.Hanson, T.B.Mcananey, E.S.Park, M.E.P.Rendell, D.K.Yarbrough, S.Chu, L.Xi, S.G.Boxer, M.H.Montrose, S.J.Remington, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.90 / 1.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.200, 62.374, 68.967, 90.00, 90.00, 90.00
R / Rfree (%)	17.7 / n/a

The binding sites of Magnesium atom in the Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH (pdb code 1jbz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH, PDB code: 1jbz:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg250 b:60.6 occ:1.00 O A:HOH443 1.8 51.2 1.0 O A:HOH497 2.1 27.0 1.0 O A:HOH441 2.9 30.9 1.0 ND2 A:ASN170 2.9 19.7 1.0 O A:HOH439 3.1 30.4 1.0 O A:HOH444 3.2 30.6 1.0 O A:HOH440 3.4 41.3 1.0 O A:GLY174 3.5 22.9 1.0 CG A:ASN170 3.7 34.5 1.0 CG1 A:VAL176 3.8 30.4 1.0 O A:SER175 3.9 19.3 1.0 O A:HOH321 3.9 20.1 1.0 O A:HOH362 4.1 43.0 1.0 CB A:ASN170 4.2 17.4 1.0 O A:HOH442 4.3 28.1 1.0 C A:GLY174 4.4 24.8 1.0 OD1 A:ASN170 4.6 49.3 1.0 O A:HOH452 4.6 45.8 1.0 CA A:ASN170 4.6 15.0 1.0 C A:SER175 4.7 20.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Analysis of A Dual-Wavelength Emission Green Fluorescent Protein Variant at High pH within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg251 b:42.4 occ:1.00 NH2 A:ARG80 2.7 50.7 1.0 OD2 A:ASP197 2.7 29.0 1.0 OD1 A:ASP197 2.9 39.0 1.0 CG A:ASP197 3.1 34.6 1.0 CZ A:ARG80 3.7 67.6 1.0 NH1 A:ARG80 3.9 60.1 1.0 CB A:ASP197 4.6 20.6 1.0 O A:HOH483 4.8 45.0 1.0 CE1 A:HIS81 4.8 27.6 1.0 NE A:ARG80 4.9 53.5 1.0

G.T.Hanson, T.B.Mcananey, E.S.Park, M.E.P.Rendell, D.K.Yarbrough, S.Chu, L.Xi, S.G.Boxer, M.H.Montrose, S.J.Remington. Green Fluorescent Protein Variants As Ratiometric Dual Emission pH Sensors. 1. Structural Characterization and Preliminary Application. Biochemistry V. 41 15477 2002.
ISSN: ISSN 0006-2960
PubMed: 12501176
DOI: 10.1021/BI026609P