Magnesium in PDB 1jqv: The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A

All present enzymatic activity of The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A:
1.3.3.1;

The structure of The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jqv was solved by S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.180, 108.370, 66.110, 90.00, 103.74, 90.00
R / Rfree (%)	18.5 / 22.8

The binding sites of Magnesium atom in the The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A (pdb code 1jqv). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jqv:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg314 b:24.1 occ:1.00 O A:LYS33 2.3 15.1 1.0 O A:HOH1448 2.5 30.6 1.0 O A:HOH1398 2.5 29.2 1.0 O A:HOH1328 2.5 14.6 1.0 O A:SER35 2.5 18.6 1.0 O A:HOH1416 2.6 26.5 1.0 C A:LYS33 3.4 17.4 1.0 C A:SER35 3.7 19.4 1.0 O A:HOH1359 4.0 18.0 1.0 O A:HOH1472 4.0 33.5 1.0 O A:ALA37 4.0 16.7 1.0 C A:ALA34 4.1 19.9 1.0 N A:SER35 4.2 19.1 1.0 O A:LEU32 4.3 17.3 1.0 CA A:LYS33 4.3 18.5 1.0 O A:ALA34 4.3 21.2 1.0 N A:ALA34 4.3 18.4 1.0 CA A:ALA34 4.4 19.0 1.0 O A:HOH1422 4.6 18.6 1.0 CA A:SER35 4.6 18.0 1.0 N A:GLN36 4.7 19.5 1.0 CA A:GLN36 4.7 20.2 1.0 C A:GLN36 4.8 18.6 1.0 N A:ALA37 4.9 16.6 1.0

Magnesium binding site 2 out of 2 in the The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The K213E Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg314 b:24.6 occ:1.00 O B:LYS33 2.3 19.0 1.0 O B:HOH2418 2.4 26.6 1.0 O B:HOH2405 2.5 31.2 1.0 O B:SER35 2.5 19.8 1.0 O B:HOH2353 2.6 17.9 1.0 C B:LYS33 3.5 19.7 1.0 C B:SER35 3.7 20.3 1.0 O B:HOH2412 3.9 18.9 1.0 O B:ALA37 4.0 14.3 1.0 C B:ALA34 4.1 20.5 1.0 O B:HOH2514 4.1 39.6 1.0 N B:SER35 4.2 19.4 1.0 O B:ALA34 4.3 21.6 1.0 O B:LEU32 4.3 17.0 1.0 N B:ALA34 4.3 19.9 1.0 CA B:LYS33 4.4 19.9 1.0 CA B:ALA34 4.4 20.0 1.0 O B:HOH2378 4.4 19.5 1.0 CA B:SER35 4.6 19.3 1.0 N B:GLN36 4.7 18.9 1.0 CA B:GLN36 4.7 20.8 1.0 C B:GLN36 4.8 17.7 1.0 N B:ALA37 4.9 17.1 1.0

S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen. Lactococcus Lactis Dihydroorotate Dehydrogenase A Mutants Reveal Important Facets of the Enzymatic Function J.Biol.Chem. V. 278 28812 2003.
ISSN: ISSN 0021-9258
PubMed: 12732650
DOI: 10.1074/JBC.M303767200