Magnesium in PDB 1jqx: The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A

All present enzymatic activity of The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A:
1.3.3.1;

The structure of The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jqx was solved by S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.350, 108.480, 66.120, 90.00, 104.03, 90.00
R / Rfree (%)	17.2 / 19.2

The binding sites of Magnesium atom in the The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A (pdb code 1jqx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jqx:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg314 b:18.1 occ:1.00 O A:LYS33 2.3 15.5 1.0 O A:SER35 2.4 14.3 1.0 O A:HOH1331 2.4 14.8 1.0 O A:HOH1421 2.5 31.1 1.0 O A:HOH1365 2.5 21.4 1.0 O A:HOH1489 2.6 29.1 1.0 C A:LYS33 3.4 15.1 1.0 C A:SER35 3.6 15.1 1.0 O A:HOH1459 3.8 18.7 1.0 C A:ALA34 4.0 15.9 1.0 O A:ALA37 4.0 13.0 1.0 N A:SER35 4.1 15.4 1.0 O A:HOH1578 4.2 37.6 1.0 O A:LEU32 4.2 13.5 1.0 O A:ALA34 4.2 16.6 1.0 N A:ALA34 4.3 15.3 1.0 CA A:LYS33 4.3 14.5 1.0 CA A:ALA34 4.3 15.4 1.0 O A:HOH1592 4.4 41.8 1.0 CA A:SER35 4.5 15.3 1.0 O A:HOH1349 4.5 22.0 1.0 N A:GLN36 4.5 14.4 1.0 CA A:GLN36 4.6 14.8 1.0 O A:HOH1628 4.6 43.7 1.0 N A:ALA37 4.7 13.8 1.0 C A:GLN36 4.7 14.3 1.0

Magnesium binding site 2 out of 2 in the The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The R57A Mutant of Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg314 b:19.9 occ:1.00 O B:HOH2554 2.3 34.2 1.0 O B:LYS33 2.4 16.8 1.0 O B:HOH2364 2.4 20.4 1.0 O B:SER35 2.4 16.6 1.0 O B:HOH2421 2.5 27.4 1.0 O B:HOH2344 2.5 16.0 1.0 C B:LYS33 3.5 16.9 1.0 C B:SER35 3.6 17.7 1.0 O B:HOH2454 3.8 19.0 1.0 O B:ALA37 4.0 16.1 1.0 O B:HOH2621 4.0 37.6 1.0 C B:ALA34 4.0 18.9 1.0 N B:SER35 4.1 18.4 1.0 O B:HOH2627 4.2 45.7 1.0 O B:ALA34 4.3 19.6 1.0 O B:LEU32 4.3 16.6 1.0 N B:ALA34 4.3 17.8 1.0 CA B:LYS33 4.4 16.8 1.0 CA B:ALA34 4.4 18.4 1.0 O B:HOH2484 4.5 32.2 1.0 CA B:SER35 4.5 17.9 1.0 O B:HOH2670 4.5 24.3 1.0 N B:GLN36 4.6 17.5 1.0 CA B:GLN36 4.6 17.7 1.0 N B:ALA37 4.8 17.2 1.0 C B:GLN36 4.8 17.3 1.0

S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen. Lactococcus Lactis Dihydroorotate Dehydrogenase A Mutants Reveal Important Facets of the Enzymatic Function J.Biol.Chem. V. 278 28812 2003.
ISSN: ISSN 0021-9258
PubMed: 12732650
DOI: 10.1074/JBC.M303767200