Magnesium in PDB 1jue: 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A

All present enzymatic activity of 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A:
1.3.3.1;

The structure of 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jue was solved by S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.146, 108.145, 66.019, 90.00, 103.82, 90.00
R / Rfree (%)	18 / 19.8

The binding sites of Magnesium atom in the 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A (pdb code 1jue). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A, PDB code: 1jue:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg850 b:18.6 occ:1.00 O A:LYS33 2.3 16.3 1.0 O A:SER35 2.4 15.7 1.0 O A:HOH1129 2.4 32.2 1.0 O A:HOH1023 2.5 14.7 1.0 O A:HOH1038 2.5 22.1 1.0 O A:HOH1115 2.5 33.0 1.0 C A:LYS33 3.4 16.2 1.0 C A:SER35 3.6 15.9 1.0 O A:ALA37 4.0 14.3 1.0 C A:ALA34 4.0 16.6 1.0 O A:HOH1099 4.0 17.3 1.0 N A:SER35 4.0 16.4 1.0 O A:HOH1275 4.1 40.4 1.0 O A:LEU32 4.2 15.5 1.0 O A:ALA34 4.2 16.9 1.0 N A:ALA34 4.3 16.3 1.0 CA A:LYS33 4.3 16.1 1.0 CA A:ALA34 4.4 16.7 1.0 CA A:SER35 4.4 16.2 1.0 N A:GLN36 4.5 15.7 1.0 O A:HOH1098 4.6 20.6 1.0 CA A:GLN36 4.6 16.0 1.0 O A:HOH1234 4.7 33.0 1.0 N A:ALA37 4.7 15.0 1.0 C A:GLN36 4.8 15.5 1.0 OG A:SER35 5.0 16.3 1.0

Magnesium binding site 2 out of 2 in the 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 1.8 A Resolution Structure of Native Lactococcus Lactis Dihydroorotate Dehydrogenase A within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg850 b:20.3 occ:1.00 O B:LYS33 2.4 17.2 1.0 O B:SER35 2.4 16.3 1.0 O B:HOH2084 2.4 39.7 1.0 O B:HOH1968 2.5 20.5 1.0 O B:HOH1923 2.5 18.2 1.0 C B:LYS33 3.5 16.9 1.0 C B:SER35 3.6 16.5 1.0 O B:HOH1988 3.9 17.4 1.0 O B:ALA37 4.0 15.2 1.0 C B:ALA34 4.0 17.1 1.0 N B:SER35 4.1 16.6 1.0 O B:HOH2038 4.2 44.0 1.0 O B:LEU32 4.3 16.6 1.0 O B:ALA34 4.3 17.1 1.0 O B:HOH2124 4.3 40.1 1.0 N B:ALA34 4.3 17.0 1.0 O B:HOH1986 4.3 22.2 1.0 CA B:LYS33 4.3 16.9 1.0 CA B:ALA34 4.4 17.1 1.0 CA B:SER35 4.5 16.4 1.0 N B:GLN36 4.5 16.3 1.0 CA B:GLN36 4.6 16.4 1.0 N B:ALA37 4.7 16.1 1.0 C B:GLN36 4.8 16.2 1.0

S.Norager, S.Arent, O.Bjornberg, M.Ottosen, L.Lo Leggio, K.F.Jensen, S.Larsen. Lactococcus Lactis Dihydroorotate Dehydrogenase A Mutants Reveal Important Facets of the Enzymatic Function J.Biol.Chem. V. 278 28812 2003.
ISSN: ISSN 0021-9258
PubMed: 12732650
DOI: 10.1074/JBC.M303767200