Magnesium in PDB 1kcz: Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex.

All present enzymatic activity of Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex.:
4.3.1.2;

The structure of Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex., PDB code: 1kcz was solved by M.Asuncion, W.Blankenfeldt, J.N.Barlow, D.Gani, J.H.Naismith, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	76.70 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	67.016, 108.783, 109.557, 90.00, 90.00, 90.00
R / Rfree (%)	13.5 / 17.5

The binding sites of Magnesium atom in the Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex. (pdb code 1kcz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex., PDB code: 1kcz:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg901 b:5.1 occ:1.00 OD2 A:ASP238 1.9 2.6 1.0 OE2 A:GLU273 1.9 7.3 1.0 O A:HOH910 2.0 11.7 1.0 O A:HOH989 2.0 14.9 1.0 O A:HOH919 2.0 7.2 1.0 OD2 A:ASP307 2.1 6.9 1.0 CG A:ASP238 2.9 4.4 1.0 CD A:GLU273 3.0 8.4 1.0 CG A:ASP307 3.1 9.7 1.0 OD1 A:ASP238 3.3 4.1 1.0 CG A:GLU273 3.6 5.0 1.0 CB A:ASP307 3.6 6.7 1.0 OE1 A:GLU273 3.9 8.0 1.0 NE2 A:GLN329 4.1 14.2 1.0 OE2 A:GLU308 4.1 13.8 1.0 OD1 A:ASP307 4.2 10.0 1.0 OE1 A:GLU308 4.3 12.0 1.0 CB A:ASP238 4.3 3.2 1.0 O A:HOH1203 4.3 47.3 1.0 O A:HOH986 4.3 18.9 1.0 NE2 A:HIS194 4.5 8.2 1.0 CD A:GLU308 4.6 11.2 1.0 CB A:GLU273 4.7 5.6 1.0 CZ A:TYR240 4.8 10.7 1.0 OH A:TYR240 4.9 13.4 1.0 CE2 A:TYR240 5.0 13.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Beta-Methylaspartase From Clostridium Tetanomorphum. Mg-Complex. within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg902 b:6.9 occ:1.00 OE2 B:GLU273 2.0 7.4 1.0 OD2 B:ASP238 2.0 4.3 1.0 O B:HOH919 2.0 5.9 1.0 OD2 B:ASP307 2.0 8.0 1.0 O B:HOH916 2.1 15.4 1.0 O B:HOH949 2.2 13.0 1.0 CD B:GLU273 3.0 9.9 1.0 CG B:ASP238 3.0 6.1 1.0 CG B:ASP307 3.1 10.1 1.0 OD1 B:ASP238 3.3 7.3 1.0 CG B:GLU273 3.6 5.7 1.0 CB B:ASP307 3.6 7.3 1.0 OE1 B:GLU273 3.9 9.4 1.0 OE1 B:GLN329 4.1 16.2 1.0 O B:HOH1173 4.1 45.0 1.0 OE2 B:GLU308 4.2 14.8 1.0 OD1 B:ASP307 4.2 8.6 1.0 OE1 B:GLU308 4.3 12.7 1.0 CB B:ASP238 4.3 3.8 1.0 O B:HOH1253 4.4 22.4 1.0 NE2 B:HIS194 4.4 6.9 1.0 O B:HOH940 4.6 14.2 1.0 CD B:GLU308 4.6 10.9 1.0 CB B:GLU273 4.7 5.8 1.0 CZ B:TYR240 4.8 10.2 1.0 OH B:TYR240 4.9 12.0 1.0 CE2 B:TYR240 5.0 11.3 1.0

M.Asuncion, W.Blankenfeldt, J.N.Barlow, D.Gani, J.H.Naismith. The Structure of 3-Methylaspartase From Clostridium Tetanomorphum Functions Via the Common Enolase Chemical Step. J.Biol.Chem. V. 277 8306 2002.
ISSN: ISSN 0021-9258
PubMed: 11748244
DOI: 10.1074/JBC.M111180200