Magnesium in PDB 1kjy: Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14

The structure of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14, PDB code: 1kjy was solved by R.J.Kimple, M.E.Kimple, L.Betts, J.Sondek, D.P.Siderovski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.70
Space group	P 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	69.510, 82.040, 187.240, 90.00, 90.00, 90.00
R / Rfree (%)	23.8 / 29.9

The structure of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 also contains other interesting chemical elements:

Caesium

(Cs)

15 atoms

The binding sites of Magnesium atom in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 (pdb code 1kjy). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14, PDB code: 1kjy:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg800 b:45.1 occ:1.00 O2B A:GDP356 2.6 32.2 1.0 OG B:SER510 2.8 90.8 1.0 O B:HOH4085 2.9 26.7 1.0 NH1 B:ARG516 3.2 46.1 1.0 CE1 B:HIS513 3.3 90.8 1.0 CB B:SER510 3.8 91.9 1.0 O A:HOH4043 3.9 36.6 1.0 NE2 B:HIS513 3.9 90.2 1.0 PB A:GDP356 4.0 32.5 1.0 O B:HOH4166 4.0 35.8 1.0 CA A:GLY42 4.1 21.9 1.0 O A:GLY202 4.1 61.1 1.0 CA B:SER510 4.3 92.2 1.0 ND1 B:HIS513 4.3 90.3 1.0 CZ B:ARG516 4.4 45.4 1.0 O3A A:GDP356 4.4 32.3 1.0 O1B A:GDP356 4.5 32.9 1.0 C A:GLY202 4.5 58.6 1.0 O B:ARG506 4.6 70.5 1.0 N A:GLU43 4.6 22.6 1.0 NZ A:LYS46 4.7 30.9 1.0 CA A:GLY202 4.8 55.0 1.0 NH2 B:ARG516 4.8 44.5 1.0 C A:GLY42 4.9 22.6 1.0 O3B A:GDP356 4.9 38.5 1.0 CG2 A:THR181 5.0 65.6 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human G[Alpha]I1 Bound to the Goloco Motif of RGS14 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg801 b:59.1 occ:1.00 O1B C:GDP355 2.5 36.4 1.0 CB C:SER1047 3.0 22.1 1.0 OD2 C:ASP1200 3.1 31.9 1.0 N C:SER1047 3.2 23.2 1.0 PB C:GDP355 3.3 30.1 1.0 O2B C:GDP355 3.4 30.6 1.0 CA C:SER1047 3.5 24.1 1.0 O3A C:GDP355 3.6 34.4 1.0 O C:HOH4178 3.8 27.3 1.0 CB C:LYS1046 3.9 17.6 1.0 CG C:ASP1200 4.0 35.0 1.0 CE C:LYS1046 4.1 15.8 1.0 OD1 C:ASP1200 4.1 33.3 1.0 OG C:SER1047 4.2 25.0 1.0 C C:LYS1046 4.2 22.4 1.0 O D:HOH4176 4.3 13.6 1.0 NZ C:LYS1046 4.3 15.5 1.0 NH1 D:ARG1516 4.4 57.7 1.0 CG2 C:THR1181 4.5 78.0 1.0 CA C:LYS1046 4.5 21.6 1.0 PA C:GDP355 4.5 34.2 1.0 O C:VAL1201 4.6 43.3 1.0 O3B C:GDP355 4.7 40.5 1.0 N C:LYS1046 4.8 20.0 1.0 CG C:LYS1046 4.9 18.0 1.0 CD C:LYS1046 5.0 17.0 1.0 C C:SER1047 5.0 23.9 1.0

R.J.Kimple, M.E.Kimple, L.Betts, J.Sondek, D.P.Siderovski. Structural Determinants For Goloco-Induced Inhibition of Nucleotide Release By Galpha Subunits. Nature V. 416 878 2002.
ISSN: ISSN 0028-0836
PubMed: 11976690
DOI: 10.1038/416878A