Magnesium in PDB 1lfd: Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds

The structure of Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds, PDB code: 1lfd was solved by L.Huang, F.Hofer, G.S.Martin, S.-H.Kim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	75.648, 78.256, 87.313, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 28.2

The binding sites of Magnesium atom in the Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds (pdb code 1lfd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds, PDB code: 1lfd:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1373 b:34.8 occ:1.00 O B:ASP233 2.8 10.7 1.0 O2B B:GNP1380 2.9 10.6 1.0 OG1 B:THR235 2.9 8.0 1.0 OG B:SER217 2.9 9.5 1.0 O2A B:GNP1380 2.9 13.5 1.0 O3G B:GNP1380 3.0 14.0 1.0 N3B B:GNP1380 3.3 7.9 1.0 CB B:SER217 3.5 9.2 1.0 PG B:GNP1380 3.6 8.7 1.0 PB B:GNP1380 3.6 8.5 1.0 N B:THR235 3.7 9.5 1.0 C B:ASP233 3.7 11.2 1.0 PA B:GNP1380 3.8 9.9 1.0 O B:HOH1400 3.9 27.4 1.0 O B:HOH1453 4.0 10.4 1.0 CA B:PRO234 4.0 8.9 1.0 O3A B:GNP1380 4.0 10.5 1.0 CB B:THR235 4.0 6.3 1.0 C B:PRO234 4.1 9.4 1.0 O B:HOH1383 4.1 18.2 1.0 O2G B:GNP1380 4.2 11.6 1.0 O1A B:GNP1380 4.3 7.4 1.0 N B:PRO234 4.3 10.7 1.0 CA B:THR235 4.4 8.6 1.0 CA B:SER217 4.8 8.8 1.0 N B:SER217 4.8 8.7 1.0 CA B:ASP233 4.9 13.6 1.0 OH D:TYR232 4.9 12.9 1.0 O1G B:GNP1380 5.0 8.3 1.0 O1B B:GNP1380 5.0 9.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Active Ras Protein Complexed with the Ras- Interacting Domain of Ralgds within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2373 b:34.0 occ:1.00 O D:ASP233 2.8 11.4 1.0 O2A D:GNP2380 2.8 15.4 1.0 OG D:SER217 2.9 11.3 1.0 OG1 D:THR235 2.9 9.9 1.0 O2B D:GNP2380 3.0 10.7 1.0 O3G D:GNP2380 3.0 15.5 1.0 CB D:SER217 3.5 7.9 1.0 N3B D:GNP2380 3.5 12.7 1.0 O D:HOH2461 3.6 46.3 1.0 PG D:GNP2380 3.7 15.4 1.0 PB D:GNP2380 3.7 12.1 1.0 PA D:GNP2380 3.7 10.8 1.0 N D:THR235 3.8 5.8 1.0 C D:ASP233 3.8 12.4 1.0 O D:HOH2397 3.8 11.2 1.0 O3A D:GNP2380 4.1 13.7 1.0 CB D:THR235 4.1 7.8 1.0 O D:HOH2382 4.1 9.8 1.0 O B:HOH1443 4.1 37.3 1.0 O1A D:GNP2380 4.2 16.5 1.0 C D:PRO234 4.2 9.2 1.0 CA D:PRO234 4.3 8.2 1.0 O2G D:GNP2380 4.4 10.7 1.0 CA D:THR235 4.5 7.6 1.0 N D:PRO234 4.5 10.1 1.0 O C:HOH125 4.5 34.4 1.0 CA D:SER217 4.8 10.3 1.0 N D:SER217 4.8 8.9 1.0 CA D:ASP233 4.9 12.2 1.0

L.Huang, F.Hofer, G.S.Martin, S.H.Kim. Structural Basis For the Interaction of Ras with Ralgds. Nat.Struct.Biol. V. 5 422 1998.
ISSN: ISSN 1072-8368
PubMed: 9628477
DOI: 10.1038/NSB0698-422