Magnesium in PDB 1lj0: Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5.

The structure of Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5., PDB code: 1lj0 was solved by A.B.Cowley, A.Altuve, O.Kuchment, S.Terzyan, X.C.Zhang, M.Rivera, D.Benson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.00 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	39.753, 50.854, 167.397, 90.00, 90.00, 90.00
R / Rfree (%)	20.7 / 25.1

The structure of Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5. also contains other interesting chemical elements:

Iron

(Fe)

4 atoms

The binding sites of Magnesium atom in the Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5. (pdb code 1lj0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5., PDB code: 1lj0:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:44.3 occ:1.00 O B:HOH210 2.2 33.5 1.0 O C:HOH222 2.2 39.0 1.0 O A:HOH334 2.2 36.4 1.0 O C:HOH220 2.3 45.1 1.0 O C:HOH221 2.4 37.4 1.0 O C:HOH214 2.4 39.6 1.0 O B:HOH223 3.9 39.7 1.0 O C:HOH239 4.1 43.8 1.0 O A:HOH345 4.2 36.1 1.0 OE2 C:GLU38 4.3 36.4 1.0 OD2 B:ASP53 4.3 24.9 1.0 OE1 A:GLU69 4.4 30.9 1.0 CB A:GLU69 4.4 27.8 1.0 O B:HOH213 4.4 31.0 1.0 CG A:GLU69 4.4 29.3 1.0 O C:SER37 4.4 36.1 1.0 OE1 C:GLU38 4.5 37.1 1.0 CG B:ASP53 4.5 24.9 1.0 OD1 A:ASP66 4.5 34.4 1.0 CD A:GLU69 4.7 30.1 1.0 CD C:GLU38 4.8 37.0 1.0 OD1 B:ASP53 4.9 22.9 1.0 CB B:ASP53 5.0 24.8 1.0

Magnesium binding site 2 out of 3 in the Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5. within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg303 b:40.0 occ:1.00 O D:HOH319 2.2 39.9 1.0 O C:HOH213 2.2 47.7 1.0 O D:HOH318 2.3 37.5 1.0 O C:HOH216 2.3 36.7 1.0 O A:HOH333 2.4 34.9 1.0 O A:HOH364 2.5 52.1 1.0 O D:HOH322 3.9 29.7 1.0 OE1 C:GLU69 3.9 33.2 1.0 OD2 D:ASP53 4.0 25.3 1.0 O D:HOH328 4.1 31.9 1.0 OE2 A:GLU38 4.2 34.5 1.0 CG D:ASP53 4.3 26.4 1.0 O C:HOH207 4.4 38.8 1.0 CG C:GLU69 4.4 32.2 1.0 OE1 A:GLU38 4.5 35.4 1.0 CB C:GLU69 4.5 29.6 1.0 CD C:GLU69 4.5 34.0 1.0 O A:SER37 4.7 31.2 1.0 CB D:ASP53 4.7 26.2 1.0 OD1 C:ASP66 4.8 30.5 1.0 CD A:GLU38 4.8 34.8 1.0 CD D:LYS85 4.8 35.7 1.0 OD1 D:ASP53 4.9 24.8 1.0 CG D:GLU56 4.9 35.3 1.0

Magnesium binding site 3 out of 3 in the Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of Quintuple Mutant of the Rat Outer Mitocondrial Cytochrome B5. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg304 b:52.9 occ:1.00 O A:HOH359 2.2 39.6 1.0 O A:HOH335 2.3 41.8 1.0 O A:HOH347 2.3 42.0 1.0 O A:HOH346 2.3 53.7 1.0 O A:HOH357 2.4 49.4 1.0 O A:HOH358 2.4 55.5 1.0 OE2 A:GLU20 4.0 30.4 1.0 OE1 A:GLU20 4.1 28.4 1.0 NH2 A:ARG15 4.2 26.5 1.0 CD A:GLU20 4.5 29.9 1.0

A.B.Cowley, A.Altuve, O.Kuchment, S.Terzyan, X.C.Zhang, M.Rivera, D.Benson. Toward Engineering the Stability and Hemin Binding Properties of Microsomal Cytochromes B5 Into Rat Outer Mitochondrial Cytochrome B5: Examining the Influence of Residues 25 and 71. Biochemistry V. 41 11566 2002.
ISSN: ISSN 0006-2960
PubMed: 12269800
DOI: 10.1021/BI026005L