Magnesium in PDB 1mb3: Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+

The structure of Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+, PDB code: 1mb3 was solved by V.Guillet, N.Ohta, S.Cabantous, A.Newton, J.-P.Samama, Structuralproteomics In Europe (Spine), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.41
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	36.770, 41.070, 67.120, 90.00, 90.00, 90.00
R / Rfree (%)	20.7 / 21.5

The binding sites of Magnesium atom in the Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+ (pdb code 1mb3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+, PDB code: 1mb3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:17.0 occ:0.41 MG A:MG201 0.0 17.0 0.4 MG A:MG201 1.7 17.0 0.4 OD2 A:ASP53 2.4 17.4 0.6 OE2 A:GLU9 2.4 14.6 1.0 O A:HOH202 2.5 17.0 0.3 OD2 A:ASP10 2.7 17.4 0.7 OD2 A:ASP53 2.9 17.8 0.0 CG A:ASP53 3.1 16.3 0.5 CD A:GLU9 3.3 11.8 1.0 CG A:ASP53 3.4 16.4 0.1 OE1 A:GLU9 3.6 15.0 1.0 OD1 A:ASP53 3.6 15.8 0.7 OD1 A:ASP53 3.7 17.2 0.1 CG A:ASP10 3.8 15.8 0.7 CB A:ASN11 4.1 12.0 1.0 CB A:ASP53 4.1 15.0 0.7 OD1 A:ASP10 4.2 17.6 0.7 N A:ASN11 4.2 10.2 1.0 ND2 A:ASN14 4.3 16.4 1.0 N A:ASP10 4.4 10.6 1.0 CB A:ASP53 4.4 15.1 0.3 O A:GLN55 4.5 18.0 1.0 CG A:GLU9 4.7 11.3 1.0 CA A:ASN11 4.9 9.4 1.0 CB A:ASP10 4.9 13.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Response Regulator Divk at pH 8.5 in Complex with MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:17.0 occ:0.44 MG A:MG201 0.0 17.0 0.4 MG A:MG201 1.7 17.0 0.4 OD2 A:ASP53 2.4 17.4 0.6 OD2 A:ASP10 2.5 17.4 0.7 OD2 A:ASP53 2.7 17.8 0.0 O A:HOH202 2.7 17.0 0.3 O A:GLN55 2.9 18.0 1.0 CG A:ASP53 3.3 16.3 0.5 CG A:ASP10 3.4 15.8 0.7 OD1 A:ASP53 3.6 15.8 0.7 CG A:ASP53 3.7 16.4 0.1 OD1 A:ASP10 3.7 17.6 0.7 C A:GLN55 3.7 16.5 1.0 OE2 A:GLU9 3.9 14.6 1.0 OD1 A:ASP53 4.0 17.2 0.1 CA A:LEU56 4.3 14.7 1.0 N A:LEU56 4.4 16.6 1.0 CB A:GLN55 4.4 18.7 1.0 CA A:GLN55 4.6 17.5 1.0 CB A:ASP53 4.7 15.0 0.7 CD A:PRO57 4.7 15.2 1.0 CB A:ASP10 4.8 13.1 1.0 CB A:ASP53 4.9 15.1 0.3 CD A:GLU9 4.9 11.8 1.0 C A:LEU56 5.0 15.0 1.0 N A:ASP10 5.0 10.6 1.0

V.Guillet, N.Ohta, S.Cabantous, A.Newton, J.-P.Samama. Crystallographic and Biochemical Studies of Divk Reveal Novel Features of An Essential Response Regulator in Caulobacter Crescentus. J.Biol.Chem. V. 277 42003 2002.
ISSN: ISSN 0021-9258
PubMed: 12176983
DOI: 10.1074/JBC.M204789200