Magnesium in PDB 1mdr: The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate

Enzymatic activity of The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate

All present enzymatic activity of The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate:
5.1.2.2;

Protein crystallography data

The structure of The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate, PDB code: 1mdr was solved by J.A.Landro, J.A.Gerlt, J.W.Kozarich, C.W.Koo, V.J.Shah, G.L.Kenyon, D.J.Neidhart, S.Fujita, G.A.Petsko, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	6.00 / 2.10
*Space group*	I 4 2 2
*Cell size a, b, c (Å), α, β, γ (°)*	125.150, 125.150, 105.650, 90.00, 90.00, 90.00
*R / R_free (%)*	15.1 / n/a

Magnesium Binding Sites:

The binding sites of Magnesium atom in the The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate (pdb code 1mdr). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate, PDB code: 1mdr:

Magnesium binding site 1 out of 1 in 1mdr

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Magnesium Binding Sites List in 1mdr

Magnesium binding site 1 out of 1 in the The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mg360 b:5.9 occ:1.00	OE2	A:GLU221	2.0	4.5	1.0
	OD2	A:ASP195	2.0	7.0	1.0
	O	A:HOH414	2.1	4.7	1.0
	O15	A:APG399	2.2	15.9	1.0
	OE1	A:GLU247	2.2	6.8	1.0
	O17	A:APG399	2.3	17.6	1.0
	C13	A:APG399	3.0	14.5	1.0
	CG	A:ASP195	3.0	8.0	1.0
	CD	A:GLU221	3.0	5.9	1.0
	CD	A:GLU247	3.1	7.8	1.0
	C12	A:APG399	3.1	17.0	1.0
	OD1	A:ASP195	3.3	8.5	1.0
	OE2	A:GLU247	3.4	5.4	1.0
	CG	A:GLU221	3.8	2.0	1.0
	OE1	A:GLU221	3.9	7.4	1.0
	C14	A:APG399	3.9	16.1	1.0
	NZ	A:LYS164	3.9	3.3	1.0
	O14	A:APG399	4.1	12.1	1.0
	O	A:HOH415	4.1	2.0	1.0
	ND2	A:ASN197	4.2	10.8	1.0
	CB	A:ASP195	4.3	6.3	1.0
	OE1	A:GLU222	4.4	9.7	1.0
	CE	A:LYS164	4.4	4.6	1.0
	CE	A:MET268	4.4	6.2	1.0
	C3	A:APG399	4.4	18.1	1.0
	CG	A:GLU247	4.4	5.3	1.0
	CD2	A:HIS297	4.8	3.1	1.0
	CG	A:ASN197	4.8	10.7	1.0
	OD1	A:ASN197	4.8	12.6	1.0
	NE2	A:HIS297	4.9	5.7	1.0
	CB	A:GLU221	4.9	2.0	1.0
	C4	A:APG399	5.0	21.2	1.0

Reference:

J.A.Landro, J.A.Gerlt, J.W.Kozarich, C.W.Koo, V.J.Shah, G.L.Kenyon, D.J.Neidhart. The Role of Lysine 166 in the Mechanism of Mandelate Racemase From Pseudomonas Putida: Mechanistic and Crystallographic Evidence For Stereospecific Alkylation By (R)-Alpha-Phenylglycidate. Biochemistry V. 33 635 1994.
ISSN: ISSN 0006-2960
PubMed: 8292591
DOI: 10.1021/BI00169A003

Page generated: Mon Dec 14 06:28:20 2020

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