Magnesium in PDB 1mus: Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna

The structure of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna, PDB code: 1mus was solved by H.M.Holden, J.B.Thoden, M.Steiniger-White, W.S.Reznikoff, S.Lovell, I.Rayment, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.90
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	112.700, 112.700, 235.900, 90.00, 90.00, 120.00
R / Rfree (%)	19 / 24.7

The structure of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna also contains other interesting chemical elements:

Manganese

(Mn)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna (pdb code 1mus). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna, PDB code: 1mus:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg480 b:15.8 occ:0.50 O A:HOH974 1.7 75.0 1.0 O A:SER178 2.5 29.0 1.0 OG A:SER181 2.6 34.0 1.0 C A:SER178 3.7 30.0 1.0 CB A:SER181 3.8 25.9 1.0 O A:HOH711 3.8 51.7 1.0 O A:HOH623 4.2 25.3 1.0 CB A:SER178 4.2 19.2 1.0 CA A:SER178 4.3 24.5 1.0 N A:MET179 4.9 23.5 1.0 CA A:SER181 4.9 23.2 1.0 N A:SER181 4.9 25.8 1.0 OG A:SER178 5.0 22.7 1.0 CA A:MET179 5.0 19.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of TN5 Transposase Complexed with Resolved Outside End Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg481 b:28.5 occ:1.00 O A:HOH726 2.0 40.6 1.0 O A:HOH730 2.1 48.6 1.0 OE1 A:GLU451 2.1 37.0 1.0 O A:HOH794 2.2 36.8 1.0 OE1 A:GLU454 2.3 39.5 1.0 O A:HOH765 2.6 58.6 1.0 CD A:GLU451 3.1 80.2 1.0 CD A:GLU454 3.2 0.0 1.0 OE2 A:GLU451 3.3 41.6 1.0 OE2 A:GLU454 3.6 74.8 1.0 O A:HOH584 4.2 51.5 1.0 CG A:GLU451 4.5 33.6 1.0 CG A:GLU454 4.6 33.8 1.0 O A:HOH850 4.8 61.5 1.0 NZ A:LYS44 4.8 78.0 1.0 CE A:LYS44 4.9 99.7 1.0 CB A:GLU451 5.0 21.2 1.0 CB A:GLU454 5.0 29.5 1.0 CA A:GLU451 5.0 17.3 1.0

M.Steiniger-White, I.Rayment, W.S.Reznikoff. Structure/Function Insights Into TN5 Transposition. Curr.Opin.Struct.Biol. V. 14 50 2004.
ISSN: ISSN 0959-440X
PubMed: 15102449
DOI: 10.1016/J.SBI.2004.01.008