Atomistry » Magnesium » PDB 1mum-1n6i » 1mxg
Atomistry »
  Magnesium »
    PDB 1mum-1n6i »
      1mxg »

Magnesium in PDB 1mxg: Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose

Enzymatic activity of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose

All present enzymatic activity of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose:
3.2.1.1;

Protein crystallography data

The structure of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose, PDB code: 1mxg was solved by A.Linden, O.Mayans, W.Meyer-Klaucke, G.Antranikian, M.Wilmanns, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.60
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 51.480, 76.507, 136.455, 90.00, 90.00, 90.00
R / Rfree (%) 15.9 / 17.2

Other elements in 1mxg:

The structure of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose also contains other interesting chemical elements:

Calcium (Ca) 1 atom
Zinc (Zn) 1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose (pdb code 1mxg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose, PDB code: 1mxg:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in 1mxg

Go back to Magnesium Binding Sites List in 1mxg
Magnesium binding site 1 out of 3 in the Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg439

b:11.9
occ:1.00
O A:ILE292 2.0 12.1 1.0
O A:HOH510 2.0 17.0 1.0
OD1 A:ASP252 2.1 14.2 1.0
OD2 A:ASP256 2.1 13.1 1.0
O A:HOH512 2.1 17.9 1.0
O A:HOH507 2.1 17.6 1.0
CG A:ASP252 3.1 16.2 1.0
CG A:ASP256 3.1 15.0 1.0
C A:ILE292 3.2 11.8 1.0
OD2 A:ASP252 3.5 16.6 1.0
CB A:ASP256 3.6 14.3 1.0
CA A:ILE293 4.0 11.6 1.0
N A:ILE293 4.1 11.5 1.0
N A:TRP294 4.1 13.9 1.0
O A:HOH881 4.2 51.9 1.0
O A:HOH854 4.2 53.3 1.0
OD1 A:ASP256 4.2 15.1 1.0
CB A:ILE292 4.2 14.5 1.0
CA A:ILE292 4.2 11.6 1.0
O A:HOH692 4.3 34.6 1.0
O A:HOH669 4.3 34.8 1.0
CB A:ASP252 4.4 12.9 1.0
O A:HOH765 4.4 42.1 1.0
O A:HOH795 4.4 49.5 1.0
CG1 A:ILE292 4.6 14.5 1.0
C A:ILE293 4.6 12.8 1.0
O A:ASP252 4.8 13.9 1.0
CA A:ASP252 4.9 13.0 1.0

Magnesium binding site 2 out of 3 in 1mxg

Go back to Magnesium Binding Sites List in 1mxg
Magnesium binding site 2 out of 3 in the Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg440

b:18.0
occ:1.00
OE2 A:GLU350 2.2 23.9 1.0
OD2 A:ASP347 2.2 21.6 1.0
O A:HOH566 2.2 26.6 1.0
OD1 A:ASP349 2.4 25.0 1.0
O A:HOH584 2.4 27.4 1.0
O A:HOH745 2.4 41.7 1.0
CG A:ASP347 2.8 19.6 1.0
OD1 A:ASP347 2.8 18.4 1.0
CD A:GLU350 3.2 20.3 1.0
CG A:ASP349 3.5 23.4 1.0
CG A:GLU350 3.5 19.1 1.0
OD2 A:ASP349 3.9 26.9 1.0
CB A:ASP347 4.2 16.3 1.0
CB A:GLU350 4.2 15.7 1.0
NH2 A:ARG377 4.3 22.0 1.0
O A:HOH673 4.3 37.3 1.0
ND2 A:ASN348 4.3 19.9 0.5
OE1 A:GLU350 4.3 21.4 1.0
N A:GLU350 4.5 14.4 1.0
O A:HOH798 4.6 50.4 1.0
N A:ASP349 4.6 16.3 1.0
OH A:TYR345 4.6 17.1 1.0
O A:HOH711 4.7 38.2 1.0
O A:HOH958 4.7 45.0 1.0
CB A:ASP349 4.7 19.4 1.0
C A:ASP349 4.8 16.4 1.0
CA A:ASP349 4.9 16.9 1.0
CE2 A:TYR345 5.0 15.4 1.0

Magnesium binding site 3 out of 3 in 1mxg

Go back to Magnesium Binding Sites List in 1mxg
Magnesium binding site 3 out of 3 in the Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Strucutre of A (Ca,Zn)-Dependent Alpha-Amylase From the Hyperthermophilic Archaeon Pyrococcus Woesei in Complex with Acarbose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg441

b:12.7
occ:1.00
O A:HOH612 2.4 27.8 1.0
O A:HOH586 2.4 26.9 1.0
O A:GLY24 2.6 13.4 1.0
OE1 A:GLU80 2.6 18.2 1.0
O A:HOH582 2.6 24.3 1.0
OE2 A:GLU88 2.6 14.5 1.0
OE1 A:GLU88 2.6 13.2 1.0
OE2 A:GLU80 2.7 18.4 1.0
CD A:GLU88 3.0 12.5 1.0
CD A:GLU80 3.0 17.4 1.0
C A:GLY24 3.6 12.4 1.0
O A:HOH486 4.1 13.8 1.0
CA A:GLY24 4.2 12.2 1.0
O A:HOH887 4.3 58.5 1.0
CA A:GLY84 4.4 11.0 1.0
CG A:GLU88 4.5 11.6 1.0
N A:SER85 4.5 11.3 1.0
CG A:GLU80 4.5 12.6 1.0
O A:GLY23 4.6 14.7 1.0
OD2 A:ASP28 4.6 16.5 1.0
N A:ILE25 4.7 12.6 1.0
O A:HOH594 4.8 27.4 1.0
C A:GLY84 4.9 10.8 1.0
CA A:ILE25 5.0 13.2 1.0

Reference:

A.Linden, O.Mayans, W.Meyer-Klaucke, G.Antranikian, M.Wilmanns. Differential Regulation of A Hyperthermophilic Alpha-Amylase with A Novel (Ca,Zn) Two-Metal Center By Zinc J.Biol.Chem. V. 278 9875 2003.
ISSN: ISSN 0021-9258
PubMed: 12482867
DOI: 10.1074/JBC.M211339200
Page generated: Mon Dec 14 06:29:49 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy