Magnesium in PDB 1mzy: Crystal Structure of Nitrite Reductase

All present enzymatic activity of Crystal Structure of Nitrite Reductase:
1.7.99.3;

The structure of Crystal Structure of Nitrite Reductase, PDB code: 1mzy was solved by H.Guo, K.Olesen, Y.Xue, J.Shapliegh, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	17.54 / 1.46
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	71.392, 71.392, 146.246, 90.00, 90.00, 120.00
R / Rfree (%)	18.5 / 21

The structure of Crystal Structure of Nitrite Reductase also contains other interesting chemical elements:

Copper

(Cu)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of Nitrite Reductase (pdb code 1mzy). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of Nitrite Reductase, PDB code: 1mzy:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of Nitrite Reductase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Nitrite Reductase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:9.1 occ:1.00 OD1 A:ASP220 2.0 8.2 1.0 O A:HOH648 2.0 12.6 1.0 OG1 A:THR224 2.1 9.3 1.0 O A:HOH632 2.2 8.5 1.0 CG A:ASP220 3.1 8.9 1.0 CB A:THR224 3.1 9.3 1.0 CB A:ASP220 3.7 9.2 1.0 CG2 A:THR224 4.0 10.5 1.0 OD1 A:ASP222 4.1 14.6 1.0 OD2 A:ASP220 4.2 9.2 1.0 O A:HOH745 4.3 22.1 1.0 OD2 A:ASP222 4.3 15.1 1.0 CA A:THR224 4.3 9.7 1.0 C A:THR224 4.4 8.6 1.0 O A:THR224 4.5 11.2 1.0 CG A:ASP222 4.6 15.1 1.0 O A:TYR225 4.6 7.7 1.0 N A:TYR225 4.8 8.1 1.0 N A:THR224 4.9 10.6 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of Nitrite Reductase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Nitrite Reductase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:11.6 occ:1.00 O A:HOH638 2.0 12.2 1.0 O A:HOH792 2.1 14.2 1.0 O A:HOH870 2.1 11.3 1.0 O A:HOH909 4.0 22.9 1.0 O A:HOH800 4.1 12.2 1.0 O A:HOH794 4.2 19.6 1.0 OD2 A:ASP294 4.3 7.0 1.0 O A:HOH630 4.3 10.8 1.0 CG A:ASP294 4.3 8.1 1.0 O A:HOH661 4.4 12.9 1.0 CB A:ASP294 4.5 5.4 1.0 O A:HOH815 4.5 34.6 1.0 OD1 A:ASP294 4.9 7.2 1.0 O A:HOH803 5.0 15.6 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of Nitrite Reductase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Nitrite Reductase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg503 b:18.9 occ:1.00 O A:HOH853 1.8 28.6 1.0 O A:HOH725 2.1 19.1 1.0 O A:HOH644 2.2 10.6 1.0 O A:HOH851 2.2 23.9 1.0 O A:HOH810 2.4 35.2 1.0 O A:HOH701 3.8 12.6 1.0 O A:HOH825 3.8 28.8 1.0 OD1 A:ASP96 4.0 10.0 1.0 O A:HOH906 4.0 24.2 1.0 OD2 A:ASP96 4.0 11.1 1.0 O A:HOH834 4.2 28.0 1.0 OE1 A:GLU74 4.4 14.4 1.0 O A:GLU74 4.4 7.6 1.0 CG A:ASP96 4.4 11.1 1.0 O A:HOH861 4.7 34.7 1.0 O A:HOH857 4.9 33.3 1.0

H.Guo, K.Olesen, Y.Xue, J.Shapliegh, L.Sjolin. The High Resolution Crystal Structures of Nitrite Reductase and Its Mutant MET182THR From Rhodobacter Sphaeroides Reveal A Gating Mechanism For the Electron Transfer to the Type 1 Copper Center To Be Published.