Magnesium in PDB 1n77: Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp.

All present enzymatic activity of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp.:
6.1.1.17;

The structure of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp., PDB code: 1n77 was solved by S.Sekine, O.Nureki, D.Y.Dubois, S.Bernier, R.Chenevert, J.Lapointe, D.G.Vassylyev, S.Yokoyama, Riken Structuralgenomics/Proteomics Initiative (Rsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.40 / 2.40
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	110.689, 219.124, 135.215, 90.00, 90.00, 90.00
R / Rfree (%)	21.3 / 25.7

The binding sites of Magnesium atom in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp. (pdb code 1n77). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp., PDB code: 1n77:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg700 b:38.4 occ:1.00 O C:HOH1386 3.2 35.7 1.0 O C:HOH1225 3.4 29.4 1.0 OP2 C:G522 3.9 35.9 1.0 N7 C:A546 4.0 37.6 1.0 N7 C:A545 4.0 35.5 1.0 N6 C:A546 4.3 33.6 1.0 O2 C:C509 4.3 35.0 1.0 OP2 C:A546 4.4 44.1 1.0 C8 C:A545 4.6 36.9 1.0 C5 C:A546 4.7 35.8 1.0 N3 C:C509 4.7 32.6 1.0 C5 C:A545 4.8 35.8 1.0 O C:HOH1335 4.8 29.6 1.0 C8 C:A546 4.9 38.3 1.0 C6 C:A546 4.9 35.1 1.0 N6 C:A545 5.0 34.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Atp. within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg701 b:39.5 occ:1.00 N7 D:A545 3.7 40.4 1.0 N7 D:A546 3.9 43.0 1.0 O D:HOH1218 4.0 29.7 1.0 O2 D:C509 4.0 42.7 1.0 C8 D:A545 4.3 42.0 1.0 OP2 D:G522 4.3 41.4 1.0 C5 D:A545 4.5 38.2 1.0 N3 D:C509 4.6 45.2 1.0 OP2 D:A546 4.6 48.8 1.0 N6 D:A546 4.6 39.6 1.0 C8 D:A546 4.7 43.7 1.0 C2 D:C509 4.7 44.8 1.0 C5 D:A546 4.7 42.2 1.0 N6 D:A545 4.8 32.2 1.0 C6 D:A545 5.0 36.0 1.0

S.Sekine, O.Nureki, D.Y.Dubois, S.Bernier, R.Chenevert, J.Lapointe, D.G.Vassylyev, S.Yokoyama. Atp Binding By Glutamyl-Trna Synthetase Is Switched to the Productive Mode By Trna Binding Embo J. V. 22 676 2003.
ISSN: ISSN 0261-4189
PubMed: 12554668
DOI: 10.1093/EMBOJ/CDG053