Magnesium in PDB 1n78: Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp.

All present enzymatic activity of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp.:
6.1.1.17;

The structure of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp., PDB code: 1n78 was solved by S.Sekine, O.Nureki, D.Y.Dubois, S.Bernier, R.Chenevert, J.Lapointe, D.G.Vassylyev, S.Yokoyama, Riken Structuralgenomics/Proteomics Initiative (Rsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.36 / 2.10
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	110.492, 219.870, 135.119, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 25.9

The binding sites of Magnesium atom in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp. (pdb code 1n78). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp., PDB code: 1n78:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg700 b:29.1 occ:1.00 O C:HOH2319 1.8 22.0 1.0 O C:HOH2320 1.9 24.1 1.0 O C:HOH2321 2.1 34.7 1.0 O C:HOH2322 2.1 32.1 1.0 O C:HOH2317 2.2 23.2 1.0 O C:HOH2318 2.4 28.3 1.0 N7 C:A546 4.0 28.8 1.0 N7 C:A545 4.0 28.0 1.0 OP2 C:G522 4.1 31.9 1.0 O C:HOH1366 4.2 32.0 1.0 OP2 C:A546 4.3 35.5 1.0 O2 C:C509 4.3 31.2 1.0 O C:HOH1079 4.4 19.8 1.0 C8 C:A545 4.4 29.6 1.0 O C:HOH1211 4.4 35.8 1.0 N6 C:A546 4.6 25.1 1.0 N3 C:C509 4.6 28.3 1.0 O C:HOH1369 4.7 44.9 1.0 C8 C:A546 4.8 29.8 1.0 C5 C:A545 4.8 28.3 1.0 C5 C:A546 4.8 27.1 1.0 C2 C:C509 4.9 30.3 1.0 O C:HOH1394 5.0 29.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Thermus Thermophilus Glutamyl-Trna Synthetase Complexed with Trna(Glu) and Glutamol-Amp. within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg701 b:33.7 occ:1.00 O D:HOH2324 1.9 46.9 1.0 O D:HOH2323 2.0 30.7 1.0 O D:HOH2328 2.0 26.7 1.0 O D:HOH2326 2.0 27.0 1.0 O D:HOH2325 2.1 30.1 1.0 O D:HOH2327 2.1 27.2 1.0 O D:HOH1085 3.9 30.0 1.0 O D:HOH1343 3.9 36.5 1.0 O D:HOH1197 4.0 32.0 1.0 OP2 D:G522 4.1 34.1 1.0 N7 D:A545 4.2 32.9 1.0 O D:HOH1409 4.3 28.1 1.0 N7 D:A546 4.3 27.9 1.0 O2 D:C509 4.5 30.8 1.0 OP2 D:A546 4.5 31.7 1.0 C8 D:A545 4.7 34.0 1.0 N3 D:C509 4.8 28.7 1.0 N6 D:A546 4.9 26.9 1.0

S.Sekine, O.Nureki, D.Y.Dubois, S.Bernier, R.Chenevert, J.Lapointe, D.G.Vassylyev, S.Yokoyama. Atp Binding By Glutamyl-Trna Synthetase Is Switched to the Productive Mode By Trna Binding Embo J. V. 22 676 2003.
ISSN: ISSN 0261-4189
PubMed: 12554668
DOI: 10.1093/EMBOJ/CDG053