Magnesium in PDB 1nsy: Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis

All present enzymatic activity of Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis:
6.3.5.1;

The structure of Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis, PDB code: 1nsy was solved by M.Rizzi, C.Nessi, M.Bolognesi, A.Galizzi, A.Coda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.300, 87.800, 61.400, 90.00, 110.60, 90.00
R / Rfree (%)	17.4 / 23.8

The binding sites of Magnesium atom in the Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis (pdb code 1nsy). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis, PDB code: 1nsy:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg6241 b:21.3 occ:1.00 O1P A:AMP4000 2.1 15.8 1.0 OD2 A:ASP50 2.1 19.9 1.0 O1 A:POP6000 2.1 18.9 1.0 O5 A:POP6000 2.2 20.6 1.0 O A:HOH6080 2.3 16.8 1.0 OE2 A:GLU162 2.3 29.5 1.0 CG A:ASP50 3.2 15.2 1.0 CD A:GLU162 3.2 35.0 1.0 P1 A:POP6000 3.3 23.0 1.0 P2 A:POP6000 3.4 20.6 1.0 P A:AMP4000 3.4 21.2 1.0 O A:POP6000 3.4 16.6 1.0 OE1 A:GLU162 3.5 23.1 1.0 CB A:ASP50 3.8 16.9 1.0 O5' A:AMP4000 3.9 18.3 1.0 O3P A:AMP4000 4.0 31.2 1.0 O3 A:POP6000 4.1 18.2 1.0 O6 A:POP6000 4.1 21.7 1.0 C5' A:AMP4000 4.2 15.3 1.0 O A:HOH6018 4.2 15.8 1.0 O3' A:AMP4000 4.2 17.1 1.0 OD1 A:ASP50 4.3 17.3 1.0 O2 A:POP6000 4.3 22.1 1.0 CE1 A:HIS159 4.4 10.6 1.0 O2P A:AMP4000 4.4 29.4 1.0 NZ A:LYS186 4.5 14.8 1.0 OG1 A:THR157 4.5 17.8 1.0 O4 A:POP6000 4.6 18.7 1.0 CG A:GLU162 4.6 15.1 1.0 C3' A:AMP4000 4.8 14.2 1.0 ND1 A:HIS159 5.0 14.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg6240 b:25.3 occ:1.00 O5 B:POP7000 2.0 15.4 1.0 O B:HOH6216 2.1 20.9 1.0 O1 B:POP7000 2.1 26.3 1.0 OD2 B:ASP50 2.2 21.0 1.0 O1P B:AMP5000 2.2 27.0 1.0 OE2 B:GLU162 2.2 27.3 1.0 CG B:ASP50 3.2 20.1 1.0 CD B:GLU162 3.2 41.5 1.0 P2 B:POP7000 3.3 18.8 1.0 P1 B:POP7000 3.3 25.2 1.0 O B:POP7000 3.4 23.9 1.0 P B:AMP5000 3.5 29.2 1.0 OE1 B:GLU162 3.5 37.0 1.0 CB B:ASP50 3.7 16.0 1.0 O3P B:AMP5000 4.0 23.1 1.0 O5' B:AMP5000 4.0 23.3 1.0 O B:HOH6038 4.1 22.5 1.0 O6 B:POP7000 4.2 11.6 1.0 C5' B:AMP5000 4.2 16.0 1.0 O3 B:POP7000 4.2 26.3 1.0 CE1 B:HIS159 4.3 13.6 1.0 OD1 B:ASP50 4.3 20.8 1.0 O2 B:POP7000 4.3 27.3 1.0 O3' B:AMP5000 4.3 23.7 1.0 OG1 B:THR157 4.4 16.9 1.0 O4 B:POP7000 4.5 20.7 1.0 CG B:GLU162 4.6 20.0 1.0 NZ B:LYS186 4.6 16.6 1.0 O2P B:AMP5000 4.6 41.0 1.0 ND1 B:HIS159 4.9 18.6 1.0

M.Rizzi, C.Nessi, A.Mattevi, A.Coda, M.Bolognesi, A.Galizzi. Crystal Structure of NH3-Dependent Nad+ Synthetase From Bacillus Subtilis. Embo J. V. 15 5125 1996.
ISSN: ISSN 0261-4189
PubMed: 8895556