Magnesium in PDB 1oyj: Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione.

All present enzymatic activity of Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione.:
2.5.1.18;

The structure of Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione., PDB code: 1oyj was solved by D.P.Dixon, A.G.Mcewen, A.J.Lapthorn, R.Edwards, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	76.829, 91.129, 165.034, 90.00, 90.00, 90.00
R / Rfree (%)	17.8 / 22.7

The structure of Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione. also contains other interesting chemical elements:

Chlorine

(Cl)

20 atoms

The binding sites of Magnesium atom in the Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione. (pdb code 1oyj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione., PDB code: 1oyj:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg815 b:38.0 occ:1.00 O32 A:GSH800 2.1 33.6 1.0 C3 A:GSH800 3.2 36.2 1.0 CA3 A:GSH800 3.8 35.9 1.0 O A:HOH888 4.0 40.2 1.0 O A:HOH849 4.1 28.7 1.0 O31 A:GSH800 4.3 36.5 1.0 CG A:GLU139 4.5 24.0 1.0 O A:HOH850 4.6 30.5 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione. within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg816 b:41.0 occ:1.00 O C:HOH843 2.0 30.7 1.0 O C:HOH867 2.1 36.4 1.0 O32 C:GSH803 2.2 37.7 1.0 O C:HOH963 2.2 41.4 1.0 O C:HOH888 2.2 30.8 1.0 C3 C:GSH803 3.3 35.5 1.0 O C:HOH883 3.8 33.7 1.0 CA3 C:GSH803 3.8 33.8 1.0 O C:HOH967 3.9 47.4 1.0 O C:HOH934 4.1 47.5 1.0 O C:HOH864 4.1 32.1 1.0 O D:HOH866 4.2 35.0 1.0 O31 C:GSH803 4.3 33.7 1.0 O C:HOH841 4.3 29.3 1.0 CG C:GLU139 4.4 23.8 1.0 O C:HOH839 4.5 30.4 1.0 O C:HOH929 4.5 46.6 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure Solution of Rice GST1 (OSGSTU1) in Complex with Glutathione. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg817 b:60.8 occ:1.00 O A:HOH927 1.8 56.7 1.0 O A:HOH915 2.1 55.4 1.0 O A:HOH968 2.3 50.4 1.0 O A:HOH950 2.3 47.4 1.0 O A:HOH969 2.4 56.5 1.0 O A:VAL190 4.2 23.3 1.0 OE2 A:GLU145 4.4 21.5 1.0 CD A:ARG142 4.4 22.2 1.0 O A:HOH905 4.5 31.6 1.0 O A:HOH935 4.6 67.3 1.0 O A:HOH917 4.6 54.0 1.0 CG A:ARG142 4.7 21.1 1.0 NE A:ARG142 4.7 27.8 1.0

D.P.Dixon, A.G.Mcewen, A.J.Lapthorn, R.Edwards. Forced Evolution of A Herbicide Detoxifying Glutathione Transferase. J.Biol.Chem. V. 278 23930 2003.
ISSN: ISSN 0021-9258
PubMed: 12692133
DOI: 10.1074/JBC.M303620200