Magnesium in PDB 1q3b: Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution.

The structure of Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution., PDB code: 1q3b was solved by G.Golan, D.O.Zharkov, H.Feinberg, A.S.Fernandes, E.I.Zaika, J.H.Kycia, A.P.Grollman, G.Shoham, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.21 / 2.05
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	57.722, 80.051, 169.311, 90.00, 90.00, 90.00
R / Rfree (%)	22.8 / 26

The structure of Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution. also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Magnesium atom in the Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution. (pdb code 1q3b). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution., PDB code: 1q3b:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:29.5 occ:1.00 O A:HIS262 1.9 41.7 1.0 O A:HOH530 2.3 40.0 1.0 CE1 A:HIS184 2.3 49.8 1.0 NE2 A:HIS184 2.4 49.0 1.0 C A:HIS262 2.7 41.2 1.0 OXT A:HIS262 2.9 41.2 1.0 ND1 A:HIS184 3.6 48.6 1.0 CD2 A:HIS262 3.6 43.0 1.0 CD2 A:HIS184 3.7 48.0 1.0 CG A:HIS262 4.1 41.0 1.0 CA A:HIS262 4.1 40.4 1.0 NE2 A:HIS262 4.2 43.3 1.0 CG A:HIS184 4.3 47.8 1.0 CB A:HIS262 4.4 39.7 1.0 CD2 A:LEU180 4.5 40.1 1.0 ND1 A:HIS262 4.8 42.0 1.0 CE1 A:HIS262 4.9 42.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Dna Repair Enzyme Endonuclease-VIII (Nei) From E. Coli: the R252A Mutant at 2.05 Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:20.9 occ:1.00 NE2 A:HIS220 2.0 56.5 1.0 O A:HOH468 2.3 29.9 1.0 CD2 A:HIS220 2.9 56.2 1.0 CE1 A:HIS220 3.1 56.2 1.0 CG A:HIS220 4.1 55.6 1.0 ND1 A:HIS220 4.1 55.5 1.0 CB A:HIS221 4.5 47.4 1.0

G.Golan, D.O.Zharkov, H.Feinberg, A.S.Fernandes, E.I.Zaika, J.H.Kycia, A.P.Grollman, G.Shoham. Structure of the Uncomplexed Dna Repair Enzyme Endonuclease VIII Indicates Significant Interdomain Flexibility. Nucleic Acids Res. V. 33 5006 2005.
ISSN: ISSN 0305-1048
PubMed: 16145054
DOI: 10.1093/NAR/GKI796