Magnesium in PDB 1qae: The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster

All present enzymatic activity of The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster:
3.1.30.2;

The structure of The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster, PDB code: 1qae was solved by M.D.Miller, K.L.Krause, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.05
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	106.700, 74.500, 68.900, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 21

The binding sites of Magnesium atom in the The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster (pdb code 1qae). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster, PDB code: 1qae:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg251 b:16.9 occ:1.00 OD1 A:ASN119 2.1 17.8 1.0 O A:HOH256 2.2 12.9 1.0 O A:HOH254 2.2 12.1 1.0 O A:HOH253 2.2 15.0 1.0 O A:HOH252 2.2 18.2 1.0 O A:HOH255 2.3 16.1 1.0 CG A:ASN119 3.3 14.9 1.0 N A:HIS89 3.9 9.4 1.0 ND2 A:ASN119 4.0 15.8 1.0 OE2 A:GLU127 4.1 13.6 1.0 OE1 A:GLU127 4.1 15.1 1.0 O A:ASN119 4.2 14.9 1.0 O A:HOH287 4.2 13.6 1.0 NE2 A:GLN114 4.2 8.4 1.0 CD1 A:TRP123 4.4 8.7 1.0 O A:HIS89 4.4 10.2 1.0 ND1 A:HIS89 4.4 13.3 1.0 CA A:GLY88 4.4 10.2 1.0 CB A:HIS89 4.4 10.5 1.0 CB A:ASN119 4.5 12.3 1.0 CD A:GLU127 4.5 13.2 1.0 C A:GLY88 4.6 9.0 1.0 CA A:HIS89 4.6 10.4 1.0 CA A:ASN119 4.7 12.7 1.0 C A:ASN119 4.8 14.3 1.0 NE1 A:TRP123 4.8 10.1 1.0 CG A:HIS89 4.9 11.5 1.0 C A:HIS89 5.0 10.3 1.0

Magnesium binding site 2 out of 2 in the The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg251 b:17.7 occ:1.00 O B:HOH254 2.2 12.7 1.0 O B:HOH256 2.2 13.4 1.0 OD1 B:ASN119 2.2 17.9 1.0 O B:HOH252 2.2 16.7 1.0 O B:HOH253 2.2 16.0 1.0 O B:HOH255 2.2 17.5 1.0 CG B:ASN119 3.4 16.2 1.0 OE1 B:GLU127 3.9 16.1 1.0 OE2 B:GLU127 3.9 14.9 1.0 N B:HIS89 4.0 9.1 1.0 O B:ASN119 4.1 15.4 1.0 ND2 B:ASN119 4.1 16.1 1.0 NE2 B:GLN114 4.1 10.4 1.0 O B:HOH288 4.2 13.8 1.0 CD1 B:TRP123 4.3 9.7 1.0 CD B:GLU127 4.4 14.4 1.0 O B:HIS89 4.5 9.3 1.0 CB B:ASN119 4.5 15.1 1.0 CB B:HIS89 4.5 11.4 1.0 CA B:GLY88 4.6 11.1 1.0 ND1 B:HIS89 4.6 13.9 1.0 CA B:ASN119 4.7 14.8 1.0 C B:ASN119 4.7 15.5 1.0 C B:GLY88 4.8 10.0 1.0 NE1 B:TRP123 4.8 10.7 1.0 CA B:HIS89 4.8 10.2 1.0

M.D.Miller, J.Cai, K.L.Krause. The Active Site of Serratia Endonuclease Contains A Conserved Magnesium-Water Cluster. J.Mol.Biol. V. 288 975 1999.
ISSN: ISSN 0022-2836
PubMed: 10329193
DOI: 10.1006/JMBI.1999.2729