Magnesium in PDB 1qm4: Methionine Adenosyltransferase Complexed with A L-Methionine Analogue

All present enzymatic activity of Methionine Adenosyltransferase Complexed with A L-Methionine Analogue:
2.5.1.6;

The structure of Methionine Adenosyltransferase Complexed with A L-Methionine Analogue, PDB code: 1qm4 was solved by B.Gonzalez, M.A.Pajares, J.A.Hermoso, J.Sanz-Aparicio, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.66
Space group	P 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	115.200, 115.200, 159.980, 90.00, 90.00, 90.00
R / Rfree (%)	23 / 29

The structure of Methionine Adenosyltransferase Complexed with A L-Methionine Analogue also contains other interesting chemical elements:

Potassium

(K)

3 atoms

The binding sites of Magnesium atom in the Methionine Adenosyltransferase Complexed with A L-Methionine Analogue (pdb code 1qm4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Methionine Adenosyltransferase Complexed with A L-Methionine Analogue, PDB code: 1qm4:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Methionine Adenosyltransferase Complexed with A L-Methionine Analogue


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Methionine Adenosyltransferase Complexed with A L-Methionine Analogue within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg404 b:32.6 occ:1.00 OXT A:AMB401 1.9 35.4 1.0 OD2 A:ASP180 2.7 28.6 1.0 C A:AMB401 3.0 43.3 1.0 O A:AMB401 3.4 42.6 1.0 CD A:PRO31 3.6 18.6 1.0 CG A:ASP180 3.6 23.6 1.0 OD1 A:ASP180 3.8 25.9 1.0 O A:GLY255 4.1 49.3 1.0 CG A:PRO31 4.1 16.1 1.0 CB A:AMB401 4.3 43.6 1.0 CA A:AMB401 4.3 43.9 1.0 CD2 A:HIS30 4.3 21.4 1.0 CZ A:PHE251 4.5 33.6 1.0 CA A:GLY258 4.5 51.2 1.0 N A:GLY258 4.5 49.5 1.0 NE2 A:HIS30 4.7 23.0 1.0 CG A:HIS30 4.9 21.7 1.0 CA A:HIS30 5.0 15.6 1.0 N A:PRO31 5.0 16.2 1.0 C A:GLY255 5.0 50.1 1.0

Magnesium binding site 2 out of 2 in the Methionine Adenosyltransferase Complexed with A L-Methionine Analogue


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Methionine Adenosyltransferase Complexed with A L-Methionine Analogue within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg405 b:51.0 occ:1.00 O2 A:SO4402 2.9 49.8 1.0 OG A:SER248 3.0 44.2 1.0 O3 A:SO4402 3.2 51.2 1.0 S A:SO4402 3.5 54.3 1.0 OD1 B:ASP135 3.6 49.6 1.0 CD1 B:ILE323 4.1 40.0 1.0 CB A:SER248 4.3 39.9 1.0 O4 A:SO4402 4.4 49.3 1.0 O1 A:SO4402 4.6 51.5 1.0 CG B:ASP135 4.8 48.5 1.0 N A:SER248 4.9 38.4 1.0 CB A:PRO247 4.9 31.7 1.0

B.Gonzalez, M.A.Pajares, J.A.Hermoso, L.Alvarez, F.Garrido, J.R.Sufrin, J.Sanz-Aparicio. The Crystal Structure of Tetrameric Methionine Adenosyltransferase From Rat Liver Reveals the Methionine-Binding Site J.Mol.Biol. V. 300 363 2000.
ISSN: ISSN 0022-2836
PubMed: 10873471
DOI: 10.1006/JMBI.2000.3858