Magnesium in PDB 1r8b: Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide

All present enzymatic activity of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide:
2.7.7.25;

The structure of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide, PDB code: 1r8b was solved by Y.Xiong, F.Li, J.Wang, A.M.Weiner, T.A.Steitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.63 / 2.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	86.402, 79.371, 78.135, 90.00, 97.33, 90.00
R / Rfree (%)	18.1 / 22.8

The structure of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide also contains other interesting chemical elements:

Manganese	(Mn)	2 atoms
Chlorine	(Cl)	1 atom
Sodium	(Na)	1 atom

The binding sites of Magnesium atom in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide (pdb code 1r8b). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide, PDB code: 1r8b:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg602 b:96.4 occ:1.00 OE2 A:GLU59 2.2 39.2 1.0 ND1 A:HIS101 2.6 41.8 1.0 O A:HOH719 2.6 76.6 1.0 OD2 A:ASP110 2.6 39.2 1.0 OD1 A:ASP110 2.7 31.7 1.0 CG A:ASP110 3.0 35.5 1.0 CE1 A:HIS101 3.0 42.1 1.0 CD A:GLU59 3.0 36.6 1.0 OE1 A:GLU59 3.6 36.0 1.0 CG A:HIS101 3.9 39.3 1.0 NH1 A:ARG93 3.9 44.9 1.0 CG A:GLU59 4.1 36.2 1.0 NE2 A:HIS101 4.3 40.7 1.0 CB A:ASP110 4.5 29.4 1.0 CB A:HIS101 4.5 34.9 1.0 OE1 A:GLU108 4.6 43.0 1.0 CD2 A:HIS101 4.7 41.9 1.0 O A:VAL109 4.7 27.9 1.0 MG A:MG604 4.8 62.1 1.0 C A:VAL109 5.0 29.7 1.0 O A:HOH750 5.0 70.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg604 b:62.1 occ:1.00 O A:HOH778 2.5 48.1 0.5 MN A:MN600 2.6 36.3 1.0 OD1 A:ASP61 2.6 29.4 1.0 OE1 A:GLU59 2.7 36.0 1.0 O A:HOH750 2.7 70.7 1.0 O1A A:ATP501 2.9 29.8 1.0 CD A:GLU59 3.2 36.6 1.0 OD2 A:ASP61 3.3 28.6 1.0 CG A:GLU59 3.4 36.2 1.0 CG A:ASP61 3.4 28.6 1.0 OD2 A:ASP110 3.7 39.2 1.0 O3G A:ATP501 3.7 38.3 1.0 CB A:GLU59 3.9 31.7 1.0 OE2 A:GLU59 4.2 39.2 1.0 PA A:ATP501 4.4 37.9 1.0 O2B A:ATP501 4.7 30.3 1.0 MG A:MG602 4.8 96.4 1.0 CG A:ASP110 4.8 35.5 1.0 CB A:ASP61 4.9 27.6 1.0

Y.Xiong, F.Li, J.Wang, A.M.Weiner, T.A.Steitz. Crystal Structures of An Archaeal Class I Cca-Adding Enzyme and Its Nucleotide Complexes Mol.Cell V. 12 1165 2003.
ISSN: ISSN 1097-2765
PubMed: 14636575
DOI: 10.1016/S1097-2765(03)00440-4