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Magnesium in PDB 1rep: Crystal Structure of Replication Initiator Protein REPE54 of Mini-F Plasmid Complexed with An Iteron Dna

Protein crystallography data

The structure of Crystal Structure of Replication Initiator Protein REPE54 of Mini-F Plasmid Complexed with An Iteron Dna, PDB code: 1rep was solved by H.Komori, F.Matsunaga, Y.Higuchi, M.Ishiai, C.Wada, K.Miki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 6.00 / 2.60
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 108.435, 81.853, 73.891, 90.00, 121.53, 90.00
R / Rfree (%) 21.3 / 27.4

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Replication Initiator Protein REPE54 of Mini-F Plasmid Complexed with An Iteron Dna (pdb code 1rep). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Replication Initiator Protein REPE54 of Mini-F Plasmid Complexed with An Iteron Dna, PDB code: 1rep:

Magnesium binding site 1 out of 1 in 1rep

Go back to Magnesium Binding Sites List in 1rep
Magnesium binding site 1 out of 1 in the Crystal Structure of Replication Initiator Protein REPE54 of Mini-F Plasmid Complexed with An Iteron Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Replication Initiator Protein REPE54 of Mini-F Plasmid Complexed with An Iteron Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg252

b:24.1
occ:1.00
O C:HOH254 1.9 17.2 1.0
O C:HOH253 1.9 12.6 1.0
O A:HOH23 1.9 14.7 1.0
O C:HOH255 2.0 22.4 1.0
OE1 C:GLU77 2.1 19.9 1.0
OD1 C:ASP81 2.1 10.1 1.0
CG C:ASP81 3.0 13.7 1.0
OD2 C:ASP81 3.2 10.6 1.0
O C:HOH268 3.2 30.4 1.0
CD C:GLU77 3.2 20.4 1.0
CG C:GLU77 3.9 23.4 1.0
OD2 C:ASP34 4.1 3.6 1.0
OE2 C:GLU77 4.2 19.2 1.0
OP2 A:DG14 4.2 30.4 1.0
CB C:ASP81 4.4 7.8 1.0
NH2 C:ARG33 4.4 16.8 1.0
O C:GLU77 4.4 30.2 1.0
N7 A:DG14 4.4 9.4 1.0
N C:ASP81 4.8 17.4 1.0
CA C:ASP81 4.8 17.3 1.0
OP2 A:DT13 4.9 20.1 1.0
C8 A:DG14 4.9 13.8 1.0
C2' A:DT13 5.0 34.2 1.0
CG C:ASP34 5.0 7.3 1.0

Reference:

H.Komori, F.Matsunaga, Y.Higuchi, M.Ishiai, C.Wada, K.Miki. Crystal Structure of A Prokaryotic Replication Initiator Protein Bound to Dna at 2.6 A Resolution. Embo J. V. 18 4597 1999.
ISSN: ISSN 0261-4189
PubMed: 10469640
DOI: 10.1093/EMBOJ/18.17.4597
Page generated: Tue Aug 13 12:59:05 2024

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