Magnesium in PDB 1rku: Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa

The structure of Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa, PDB code: 1rku was solved by S.K.Singh, K.Yang, K.Subramanian, S.Karthikeyan, T.Huynh, X.Zhang, M.A.Phillips, H.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.47
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	71.749, 97.236, 131.772, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 22.1

The binding sites of Magnesium atom in the Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa (pdb code 1rku). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa, PDB code: 1rku:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:21.5 occ:1.00 O A:HOH814 1.8 26.0 1.0 O A:HOH719 2.0 19.4 1.0 OD2 A:ASP7 2.0 24.0 1.0 O A:GLU9 2.1 22.3 1.0 O A:HOH843 2.1 25.2 1.0 OD1 A:ASP152 2.2 26.1 1.0 CG A:ASP7 3.2 21.6 1.0 CG A:ASP152 3.2 24.7 1.0 C A:GLU9 3.3 19.9 1.0 OD2 A:ASP152 3.4 24.0 1.0 OD1 A:ASP7 3.7 21.6 1.0 OD2 A:ASP156 3.9 26.7 1.0 OG A:SER153 4.0 25.1 0.5 O A:HOH600 4.0 31.9 1.0 CA A:GLU9 4.1 19.0 1.0 OE2 A:GLU15 4.2 33.8 1.0 CB A:GLU9 4.2 19.0 1.0 N A:GLU9 4.2 16.4 1.0 N A:GLY10 4.2 20.1 1.0 CB A:ASP7 4.3 16.7 1.0 CA A:GLY10 4.4 21.1 1.0 O A:HOH627 4.4 36.9 1.0 O A:HOH523 4.4 23.3 1.0 CB A:ASP152 4.6 22.2 1.0 O A:HOH689 4.7 42.3 1.0 N A:ASP152 4.8 20.6 1.0 CB A:SER153 4.8 24.4 0.5 CB A:SER153 4.8 24.2 0.5 OD1 A:ASP156 4.8 32.5 1.0 CG A:ASP156 4.8 25.6 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:34.6 occ:1.00 O A:HOH845 1.9 31.4 1.0 OD2 A:ASP152 2.1 24.0 1.0 O A:HOH725 2.3 39.0 1.0 O A:HOH844 2.5 43.0 1.0 CG A:ASP152 3.2 24.7 1.0 CB A:ASP152 3.8 22.2 1.0 O A:HOH843 3.9 25.2 1.0 O A:HIS170 4.0 24.0 1.0 CA A:GLY10 4.1 21.1 1.0 OE2 A:GLU15 4.2 33.8 1.0 OD1 A:ASP152 4.2 26.1 1.0 O A:ASP152 4.4 24.6 1.0 OG A:SER153 4.4 25.1 0.5 O A:HOH825 4.4 47.9 1.0 O A:HOH692 4.5 42.0 1.0 O A:HOH818 4.6 40.4 1.0 O A:GLY10 4.7 24.6 1.0 C A:GLY10 4.8 22.7 1.0 C A:ASP152 4.8 23.2 1.0 O A:HOH817 5.0 42.9 1.0 CA A:ASP152 5.0 22.0 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg303 b:19.1 occ:1.00 O B:HOH748 2.0 20.1 1.0 O B:HOH780 2.0 19.1 1.0 O B:GLU9 2.0 18.4 1.0 OD2 B:ASP7 2.1 21.5 1.0 O B:HOH846 2.1 23.7 1.0 OD1 B:ASP152 2.2 23.8 1.0 CG B:ASP7 3.2 19.0 1.0 CG B:ASP152 3.2 22.6 1.0 C B:GLU9 3.2 18.2 1.0 OD2 B:ASP152 3.4 20.0 1.0 OD1 B:ASP7 3.5 20.3 1.0 OE2 B:GLU15 4.0 25.7 0.5 OD2 B:ASP156 4.0 22.5 1.0 CA B:GLU9 4.1 17.2 1.0 O B:HOH588 4.1 34.4 1.0 N B:GLY10 4.2 18.2 1.0 N B:GLU9 4.2 16.4 1.0 CB B:GLU9 4.2 17.6 1.0 O B:HOH792 4.3 46.7 1.0 CB B:ASP7 4.4 18.5 1.0 CA B:GLY10 4.4 20.8 1.0 OG B:SER153 4.5 31.1 1.0 CB B:ASP152 4.5 20.6 1.0 O B:HOH871 4.5 24.6 1.0 O B:HOH858 4.7 18.0 1.0 OD1 B:ASN155 4.7 38.7 1.0 N B:ASP152 4.7 18.8 1.0 CB B:SER153 4.7 24.9 1.0 OD1 B:ASP156 4.9 25.1 1.0 CG B:ASP156 4.9 23.3 1.0 CD B:GLU15 4.9 22.9 0.5 C B:GLY10 4.9 21.2 1.0 C B:LEU8 5.0 16.4 1.0 CG2 B:VAL11 5.0 20.5 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Thrh Gene Product of Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg304 b:27.0 occ:1.00 O B:HOH813 1.9 28.8 1.0 O B:HOH847 2.0 27.9 1.0 O B:HOH840 2.0 30.5 1.0 OD2 B:ASP152 2.1 20.0 1.0 O B:HOH749 2.1 26.7 1.0 O B:HOH872 2.4 31.1 1.0 CG B:ASP152 3.1 22.6 1.0 CB B:ASP152 3.7 20.6 1.0 CA B:GLY10 3.9 20.8 1.0 OE2 B:GLU15 4.1 25.7 0.5 O B:HOH748 4.1 20.1 1.0 O B:HIS170 4.1 24.9 1.0 OD1 B:ASP152 4.2 23.8 1.0 O B:ASP152 4.3 23.3 1.0 O B:GLY10 4.3 22.9 1.0 OE2 B:GLU15 4.5 25.6 0.5 C B:GLY10 4.6 21.2 1.0 C B:ASP152 4.8 22.5 1.0 CA B:ASP152 4.9 20.9 1.0

S.K.Singh, K.Yang, S.Karthikeyan, T.Huynh, X.Zhang, M.A.Phillips, H.Zhang. The Thrh Gene Product of Pseudomonas Aeruginosa Is A Dual Activity Enzyme with A Novel Phosphoserine:Homoserine Phosphotransferase Activity. J.Biol.Chem. V. 279 13166 2004.
ISSN: ISSN 0021-9258
PubMed: 14699121
DOI: 10.1074/JBC.M311393200