Magnesium in PDB 1rrg: Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form

The structure of Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form, PDB code: 1rrg was solved by S.E.Greasley, H.Jhoti, B.Bax, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	122.360, 45.010, 91.960, 90.00, 133.62, 90.00
R / Rfree (%)	18.7 / 31.5

The binding sites of Magnesium atom in the Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form (pdb code 1rrg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form, PDB code: 1rrg:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg182 b:64.8 occ:1.00 O B:HOH223 2.3 49.3 1.0 OG1 A:THR31 2.7 26.2 1.0 O1B A:GDP183 2.7 22.9 1.0 O A:HOH253 2.8 25.4 1.0 O A:HOH246 3.0 47.9 1.0 O A:HOH255 3.5 55.2 1.0 OE1 A:GLU54 3.6 39.6 1.0 O3B A:GDP183 3.6 26.6 1.0 CB A:THR31 3.7 21.9 1.0 PB A:GDP183 3.8 24.3 1.0 CD A:GLU54 4.1 33.9 1.0 OE2 A:GLU54 4.1 38.9 1.0 O A:HOH226 4.3 65.9 1.0 O A:HOH220 4.3 45.0 1.0 O A:HOH247 4.4 41.7 1.0 O2A A:GDP183 4.4 30.4 1.0 CG2 A:THR31 4.5 20.6 1.0 O3A A:GDP183 4.6 25.3 1.0 OD1 A:ASP67 4.6 36.4 1.0 CA A:THR31 4.9 20.4 1.0 N A:THR31 4.9 20.7 1.0 OD2 A:ASP67 4.9 32.7 1.0 O2B A:GDP183 5.0 24.7 1.0

Magnesium binding site 2 out of 2 in the Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Non-Myristoylated Rat Adp-Ribosylation Factor-1 Complexed with Gdp, Dimeric Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg182 b:64.8 occ:1.00 O B:HOH259 2.3 36.8 1.0 O2B B:GDP183 2.3 20.4 1.0 OG1 B:THR31 2.5 20.4 1.0 O B:HOH260 2.6 37.0 1.0 O B:HOH254 2.8 35.2 1.0 O3B B:GDP183 3.4 20.1 1.0 PB B:GDP183 3.4 21.7 1.0 CB B:THR31 3.8 21.7 1.0 OE1 B:GLU54 3.8 40.4 1.0 OE2 B:GLU54 4.0 40.2 1.0 OD1 B:ASP67 4.1 30.6 1.0 CD B:GLU54 4.2 36.5 1.0 O B:HOH212 4.3 38.8 1.0 O1B B:GDP183 4.4 18.0 1.0 O3A B:GDP183 4.4 19.5 1.0 O A:HOH235 4.5 64.8 1.0 OD2 B:ASP67 4.5 31.9 1.0 CG2 B:THR31 4.6 23.6 1.0 O A:HOH255 4.6 55.2 1.0 O A:HOH232 4.6 46.0 1.0 N B:THR31 4.6 23.3 1.0 CA B:THR31 4.8 22.6 1.0 CG B:ASP67 4.8 29.4 1.0 O B:HOH213 4.9 35.7 1.0 O2A B:GDP183 4.9 25.8 1.0

S.E.Greasley, H.Jhoti, C.Teahan, R.Solari, A.Fensome, G.M.Thomas, S.Cockcroft, B.Bax. The Structure of Rat Adp-Ribosylation Factor-1 (Arf-1) Complexed to Gdp Determined From Two Different Crystal Forms. Nat.Struct.Biol. V. 2 797 1995.
ISSN: ISSN 1072-8368
PubMed: 7552752
DOI: 10.1038/NSB0995-797