Magnesium in PDB 1rvb: MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution

The structure of MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution, PDB code: 1rvb was solved by D.Kostrewa, F.K.Winkler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.10
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	49.160, 50.420, 64.060, 96.35, 108.85, 108.45
R / Rfree (%)	16.5 / n/a

The binding sites of Magnesium atom in the MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution (pdb code 1rvb). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution, PDB code: 1rvb:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg301 b:32.0 occ:1.00 O D:HOH16 2.1 19.7 1.0 O B:HOH342 2.1 30.2 1.0 OD1 B:ASP90 2.1 43.6 1.0 O B:HOH341 2.1 22.8 1.0 OP1 D:DA7 2.1 30.7 1.0 OD1 B:ASP74 2.9 27.6 1.0 OD2 B:ASP74 3.1 30.7 1.0 CG B:ASP90 3.1 29.4 1.0 CG B:ASP74 3.2 23.1 1.0 OD2 B:ASP90 3.4 34.6 1.0 P D:DA7 3.5 23.8 1.0 O B:HOH381 3.6 37.9 1.0 O3' D:DT6 3.8 22.4 1.0 C5' D:DA7 3.9 19.9 1.0 O D:HOH24 4.0 32.7 1.0 O5' D:DA7 4.2 25.0 1.0 N B:ASP74 4.4 19.3 1.0 CB B:ASP90 4.4 30.3 1.0 CB B:ASP74 4.5 15.8 1.0 O B:ILE91 4.5 19.1 1.0 NZ B:LYS92 4.5 30.9 1.0 OP2 D:DA7 4.6 30.6 1.0 N B:ILE91 4.6 19.6 1.0 CA B:ASP90 4.7 24.8 1.0 CA B:ASP74 4.8 22.6 1.0 O B:HOH379 5.0 29.3 1.0

Magnesium binding site 2 out of 2 in the MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of MG2+ Binding to the Active Site of Eco Rv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 Angstroms Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg302 b:41.7 occ:1.00 O B:HOH379 2.0 29.3 1.0 O B:HOH349 2.2 32.3 1.0 OE2 B:GLU45 2.2 82.3 1.0 OD1 B:ASP74 2.7 27.6 1.0 CD B:GLU45 3.3 74.4 1.0 CG B:ASP74 3.4 23.1 1.0 CB B:ASP74 3.8 15.8 1.0 OE1 B:GLU45 3.9 83.8 1.0 CA B:ASP74 4.0 22.6 1.0 CG B:GLU45 4.3 58.4 1.0 OD2 B:ASP74 4.4 30.7 1.0 O B:HOH342 4.4 30.2 1.0 O B:HOH381 4.5 37.9 1.0 O B:ASP74 4.6 23.1 1.0 OD2 A:ASP36 4.6 62.8 1.0 C B:ASP74 4.8 22.9 1.0 OE1 B:GLU65 4.9 38.9 1.0 CD B:PRO66 5.0 19.9 1.0

D.Kostrewa, F.K.Winkler. MG2+ Binding to the Active Site of Ecorv Endonuclease: A Crystallographic Study of Complexes with Substrate and Product Dna at 2 A Resolution. Biochemistry V. 34 683 1995.
ISSN: ISSN 0006-2960
PubMed: 7819264
DOI: 10.1021/BI00002A036