Magnesium in PDB 1ryf: Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase

All present enzymatic activity of Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase:
3.6.5.2;

The structure of Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase, PDB code: 1ryf was solved by M.R.Ahmadian, D.Fiegen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.92 / 1.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.640, 78.690, 96.790, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 22.3

The binding sites of Magnesium atom in the Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase (pdb code 1ryf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase, PDB code: 1ryf:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg539 b:16.8 occ:1.00 O2B A:GDP538 2.0 14.2 1.0 O A:HOH605 2.0 28.7 1.0 O A:HOH606 2.1 28.5 1.0 OG1 A:THR17 2.1 14.9 1.0 O A:HOH541 2.1 29.9 1.0 O A:HOH540 2.2 30.5 1.0 CB A:THR17 3.1 15.6 1.0 PB A:GDP538 3.2 15.8 1.0 O3B A:GDP538 3.5 12.5 1.0 N A:THR17 3.9 15.9 1.0 OD2 A:ASP57 4.0 17.7 1.0 CA A:THR17 4.1 15.3 1.0 O2A A:GDP538 4.1 13.4 1.0 CG2 A:THR17 4.2 14.2 1.0 OD1 A:ASP57 4.2 20.9 1.0 O1B A:GDP538 4.3 13.8 1.0 O3A A:GDP538 4.3 15.7 1.0 PA A:GDP538 4.5 14.5 1.0 CG A:ASP57 4.5 24.7 1.0 O A:HOH580 4.6 43.8 1.0 O1A A:GDP538 4.7 16.2 1.0 O A:HOH624 4.8 35.2 1.0 NZ A:LYS16 4.9 17.9 1.0 CB A:LYS16 4.9 14.2 1.0 O A:HOH585 4.9 36.7 1.0

Magnesium binding site 2 out of 2 in the Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1539 b:15.4 occ:1.00 O2B B:GDP1538 2.0 14.3 1.0 O B:HOH1586 2.1 31.8 1.0 O B:HOH1587 2.1 27.5 1.0 O B:HOH1585 2.1 27.4 1.0 OG1 B:THR17 2.1 14.7 1.0 O B:HOH1584 2.4 34.2 1.0 CB B:THR17 3.2 15.3 1.0 PB B:GDP1538 3.2 14.9 1.0 O3B B:GDP1538 3.5 15.9 1.0 N B:THR17 3.9 15.6 1.0 OD2 B:ASP57 4.0 22.4 1.0 O2A B:GDP1538 4.1 15.1 1.0 CA B:THR17 4.1 15.6 1.0 OD1 B:ASP57 4.1 22.9 1.0 CG2 B:THR17 4.2 15.6 1.0 O1B B:GDP1538 4.2 14.3 1.0 O B:HOH1567 4.3 40.8 1.0 O3A B:GDP1538 4.3 15.1 1.0 O B:HOH1569 4.4 58.2 1.0 PA B:GDP1538 4.5 16.2 1.0 CG B:ASP57 4.5 24.9 1.0 O B:HOH1546 4.5 42.3 1.0 O1A B:GDP1538 4.6 17.2 1.0 O B:HOH1565 4.7 36.5 1.0 O B:THR58 4.8 41.6 1.0 NZ B:LYS16 4.9 15.3 1.0 O B:HOH1663 4.9 37.6 1.0 O B:HOH1675 4.9 57.1 1.0 CB B:LYS16 5.0 14.3 1.0

D.Fiegen, L.C.Haeusler, L.Blumenstein, U.Herbrand, R.Dvorsky, I.R.Vetter, M.R.Ahmadian. Alternative Splicing of RAC1 Generates RAC1B, A Self-Activating Gtpase J.Biol.Chem. V. 279 4743 2004.
ISSN: ISSN 0021-9258
PubMed: 14625275
DOI: 10.1074/JBC.M310281200