Magnesium in PDB 1s1c: Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki

The structure of Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki, PDB code: 1s1c was solved by R.Dvorsky, L.Blumenstein, I.R.Vetter, M.R.Ahmadian, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.77 / 2.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.404, 89.537, 98.105, 90.00, 91.86, 90.00
R / Rfree (%)	21.9 / 25.7

The binding sites of Magnesium atom in the Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki (pdb code 1s1c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki, PDB code: 1s1c:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg300 b:24.5 occ:1.00 O2G A:GNP200 2.2 22.6 1.0 O A:HOH301 2.2 17.7 1.0 OG1 A:THR19 2.3 18.4 1.0 O2B A:GNP200 2.3 17.8 1.0 OG1 A:THR37 2.4 28.7 1.0 O A:HOH314 2.4 17.0 1.0 CB A:THR37 3.1 25.9 1.0 PG A:GNP200 3.2 22.9 1.0 N3B A:GNP200 3.2 21.1 1.0 PB A:GNP200 3.2 18.3 1.0 CB A:THR19 3.3 21.0 1.0 N A:THR37 3.8 25.1 1.0 CA A:THR37 4.1 26.5 1.0 O2A A:GNP200 4.1 21.1 1.0 O3G A:GNP200 4.1 22.1 1.0 N A:THR19 4.1 19.4 1.0 O1G A:GNP200 4.2 22.2 1.0 O3A A:GNP200 4.2 19.7 1.0 CG2 A:THR37 4.3 24.3 1.0 CA A:THR19 4.3 19.6 1.0 OD2 A:ASP59 4.3 26.2 1.0 CG2 A:THR19 4.3 18.9 1.0 OD1 A:ASP59 4.3 22.6 1.0 O1B A:GNP200 4.3 20.7 1.0 O A:VAL35 4.4 25.9 1.0 O A:HOH317 4.5 24.3 1.0 PA A:GNP200 4.5 20.6 1.0 CG A:ASP59 4.7 24.1 1.0 O A:THR60 4.7 21.0 1.0 C A:PRO36 4.8 25.5 1.0 O1A A:GNP200 4.8 20.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Complex Between the Human Rhoa and Rho-Binding Domain of Human Rocki within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1300 b:30.3 occ:1.00 OG1 B:THR37 2.3 31.4 1.0 O B:HOH1301 2.3 24.1 1.0 O2G B:GNP1200 2.3 30.6 1.0 O B:HOH1313 2.4 24.5 1.0 OG1 B:THR19 2.4 20.8 1.0 O2B B:GNP1200 2.4 22.9 1.0 CB B:THR37 3.2 28.9 1.0 N3B B:GNP1200 3.2 26.8 1.0 PG B:GNP1200 3.2 26.1 1.0 CB B:THR19 3.3 21.1 1.0 PB B:GNP1200 3.3 24.7 1.0 N B:THR37 3.7 32.5 1.0 O2A B:GNP1200 3.8 29.6 1.0 CA B:THR37 4.0 30.2 1.0 O B:VAL35 4.1 36.4 1.0 O1G B:GNP1200 4.2 24.4 1.0 O3A B:GNP1200 4.2 27.5 1.0 CG2 B:THR19 4.2 17.4 1.0 O3G B:GNP1200 4.3 23.3 1.0 N B:THR19 4.3 22.1 1.0 PA B:GNP1200 4.4 28.9 1.0 CG2 B:THR37 4.4 26.3 1.0 CA B:THR19 4.4 21.3 1.0 O1B B:GNP1200 4.5 26.4 1.0 OD2 B:ASP59 4.5 21.3 1.0 C B:PRO36 4.6 33.0 1.0 OD1 B:ASP59 4.6 18.2 1.0 O1A B:GNP1200 4.7 28.9 1.0 CE1 B:TYR34 4.8 45.8 1.0 CA B:PRO36 5.0 33.6 1.0 CG B:ASP59 5.0 22.1 1.0

R.Dvorsky, L.Blumenstein, I.R.Vetter, M.R.Ahmadian. Structural Insights Into the Interaction of Rocki with the Switch Regions of Rhoa. J.Biol.Chem. V. 279 7098 2004.
ISSN: ISSN 0021-9258
PubMed: 14660612
DOI: 10.1074/JBC.M311911200